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排序方式: 共有1110条查询结果,搜索用时 15 毫秒
81.
Hatem A. Elmezayen Hirohisa Okabe Yoshifumi Baba Toshihiko Yusa Rumi Itoyama Yosuke Nakao Takanobu Yamao Naoki Umzaki Masayo Tsukamoto Yuki Kitano Tatsunori Miyata Kota Arima Hiromitsu Hayashi Katsunori Imai Akira Chikamoto Yo-ichi Yamashita Hideo Baba 《Surgery today》2020,50(6):569-576
Programmed death ligand 1 (PD-L1) is a key target for the treatment of several malignancies. The present study was conducted to clarify the role of serum PD-L1 in hepatocellular carcinoma (HCC). Serum PD-L1 (sPD-L1) was examined by an enzyme-linked immunosorbent assay in 153 patients with HCC who underwent curative hepatectomy at Kumamoto University in 2011–2016. The expression of PD-L1 in tissue (tPD-L1) was investigated by immunohistochemistry. The clinical roles of the PD-L1 expression in both serum and tissue were examined. The sPD-L1 was significantly elevated in HCC patients compared to patients without any malignant or inflammatory disease (234 vs. 93 pg/mL, p < 0.0001). The percentage of the tPD-L1-positive area (%tPD-L1) in the background liver was significantly higher than in the tumor (1.52% vs. 0.48%, p < 0.0001). The %tPD-L1 in the background liver but not in the tumor was significantly correlated with the sPD-L1 level (p = 0.0079). The sPD-L1, %tPD-L1 in the tumor, and %tPD-L1 in the background liver were not correlated with the overall survival after surgery. PD-L1-expressing cells in the background liver, but not in the tumor tissue, appeared to contribute to the sPD-L1 level. The sPD-L1 level may thus not indicate the tumor burden in patients with HCC. 相似文献
82.
Differential diagnosis of kidney transplant rejection and cyclosporin/tacrolimus nephropathy using urine cytology 总被引:3,自引:0,他引:3
Masahiro Kyo Yasuji Ichikawa Kiyohide Toki Kennichi Nishimura Takanobu Fukunishi Shunsuke Nagano Yukiomi Namba Fred Gudat Peter Dalquen M J Mihatsch 《Clinical transplantation》2002,16(S8):40-44
Abstract: A total of 9000 urine samples from 69 kidney transplant recipients were studied for differential diagnoses of transplant rejection and cyclosporin/tacrolimus toxicity. New–Sternheimer and Papanicolaou staining were used to differentiate cells in urine. We also employed an immunocytochemical technique for further identification of exfoliated cells. With New–Sternheimer and Papanicolaou staining, the predominance of proximal tubular cells was useful to differentiate cyclosporin/tacrolimus toxicity from acute rejection in cases of increased serum creatinine level. During rejection episodes, an increased number of mononuclear cells and renal epithelial cells were found. Immunocytochemical analysis showed a significant increase of CD2-, CD4- CD8-, CD25- and HLA-DR-positive cells with rejection. However, there was no relationship between Banff criteria rejection grade and the increase of mononuclear cells. 相似文献
83.
84.
Tomoharu Mochizuki Takashi Sato Osamu Tanifuji Satoshi Watanabe Koichi Kobayashi Naoto Endo 《The Journal of arthroplasty》2018,33(7):2100-2110
Background
This study aimed to identify the factors affecting postoperative rotational limb alignment of the tibia relative to the femur. We hypothesized that not only component positions but also several intrinsic factors were associated with postoperative rotational limb alignment.Methods
This study included 99 knees (90 women and 9 men) with a mean age of 77 ± 6 years. A three-dimensional (3D) assessment system was applied under weight-bearing conditions to biplanar long-leg radiographs using 3D-to-2D image registration technique. The evaluation parameters were (1) component position; (2) preoperative and postoperative coronal, sagittal, and rotational limb alignment; (3) preoperative bony deformity, including femoral torsion, condylar twist angle, and tibial torsion; and (4) preoperative and postoperative range of motion (ROM).Results
In multiple linear regression analysis using a stepwise procedure, postoperative rotational limb alignment was associated with the following: (1) rotation of the component position (tibia: β = 0.371, P < .0001; femur: β = ?0.327, P < .0001), (2) preoperative rotational limb alignment (β = 0.253, P = .001), (3) postoperative flexion angle (β = 0.195, P = .007), and (4) tibial torsion (β = 0.193, P = .010).Conclusion
In addition to component positions, the intrinsic factors, such as preoperative rotational limb alignment, ROM, and tibial torsion, affected postoperative rotational limb alignment. On a premise of correct component positions, the intrinsic factors that can be controlled by surgeons should be taken care. In particular, ROM is necessary to be improved within the possible range to acquire better postoperative rotational limb alignment. 相似文献85.
Decreased susceptibility to renovascular hypertension in mice lacking the prostaglandin I2 receptor IP 总被引:2,自引:0,他引:2 下载免费PDF全文
Fujino T Nakagawa N Yuhki K Hara A Yamada T Takayama K Kuriyama S Hosoki Y Takahata O Taniguchi T Fukuzawa J Hasebe N Kikuchi K Narumiya S Ushikubi F 《The Journal of clinical investigation》2004,114(6):805-812
Persistent reduction of renal perfusion pressure induces renovascular hypertension by activating the renin-angiotensin-aldosterone system; however, the sensing mechanism remains elusive. Here we investigated the role of PGI2 in renovascular hypertension in vivo, employing mice lacking the PGI2 receptor (IP-/- mice). In WT mice with a two-kidney, one-clip model of renovascular hypertension, the BP was significantly elevated. The increase in BP in IP-/- mice, however, was significantly lower than that in WT mice. Similarly, the increases in plasma renin activity, renal renin mRNA, and plasma aldosterone in response to renal artery stenosis were all significantly lower in IP-/- mice than in WT mice. All these parameters were measured in mice lacking the four PGE2 receptor subtypes individually, and we found that these mice had similar responses to WT mice. PGI2 is produced by COX-2 and a selective inhibitor of this enzyme, SC-58125, also significantly reduced the increases in plasma renin activity and renin mRNA expression in WT mice with renal artery stenosis, but these effects were absent in IP-/- mice. When the renin-angiotensin-aldosterone system was activated by salt depletion, SC-58125 blunted the response in WT mice but not in IP-/- mice. These results indicate that PGI2 derived from COX-2 plays a critical role in regulating the release of renin and consequently renovascular hypertension in vivo. 相似文献
86.
87.
Hiroshi Saeki Takanobu Masuda Satoko Okada Koji Ando Masahiko Sugiyama Keiji Yoshinaga Kazuya Endo Noriaki Sadanaga Yasunori Emi Yoshihiro Kakeji Masaru Morita Natsumi Yamashita Yoshihiko Maehara 《Surgery today》2010,40(7):626-631
Purpose
Prediction of the postoperative course of esophagectomy is an important part of the strict perioperative management of patients undergoing surgery for esophageal cancer.Methods
To evaluate their clinical importance, peripheral blood values, including white blood cell count (WBC), lymphocyte count, and the levels of total protein, transferrin, factor XIII, D-dimer, fibrin, and fibrinogen degradation products (FDP) were measured before and after esophagectomy for esophageal cancer in 24 patients.Results
The preoperative WBC and the pre- and postoperative lymphocyte count were decreased remarkably in patients who received preoperative chemoradiotherapy. The values of perioperative serum transferrin were significantly lower in patients with postoperative pneumonia than in those without. The activity of plasma factor XIII was suppressed on postoperative day (POD) 7 in patients with pneumonia and on POD 14 in patients with leakage.Conclusions
These results suggest that patients who receive preoperative chemoradiotherapy are potentially immunosuppressed, the preoperative serum transferrin level is a possible predictive marker of postoperative pneumonia, and suppression of factor XIII activity is related to anastomotic insufficiency. 相似文献88.
Eiji Oki MD PhD Yoshihiro Kakeji MD PhD Yan Zhao MD PhD Rintaro Yoshida MD Koji Ando MD Takanobu Masuda MD Kippei Ohgaki MD PhD Masaru Morita MD PhD Yoshihiko Maehara MD PhD FACS 《Annals of surgical oncology》2009,16(9):2510-2515
Introduction Conflicting data exist regarding the relevance of high-frequency microsatellite instability (MSI-H) for predicting the prognosis
and benefits of 5-fluorouracil (5-FU)-based chemotherapy. This study investigated the usefulness of MSI as either a prognostic
indicator or predictor of distinct clinical attributes regarding the use of adjuvant chemotherapy with 5-FU and its analogues
in gastric cancer.
Materials and methods Data and tumor specimens were collected from 240 gastric cancer patients from 1993 to 2002. Five microsatellite loci were
analyzed using a high-intensity microsatellite analysis reported previously. A Cox proportional hazard model was used to compare
the clinical data and survival as well as any associations between MSI and 5-FU treatment status of patients with MSI or microsatellite
stability (MSS) gastric cancers. A 3-(4,5-dimethyl-2-thiazolyl) -2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was conducted in 168 cases to investigate chemosensitivity to 5-FU.
Results This analysis identified 22 MSI-H (9.4%), 25 MSI-L (10.7%), and 193 MSS (79.9%) tumors. Gastric cancer with MSI-H tended to
have increased likelihood to show higher age, antral location of the tumor, and lymph vessel involvement (P < 0.05). Univariate analyses failed to show any difference between the MSI-H and MSS/MSI-L groups with respect to overall
survival. Furthermore, survival after the administration of 5-FU did not correlate with MSI status, and MSI was not associated
with 5-FU sensitivity by MTT assay.
Conclusion The results of this study indicate that MSI status has no clear influence on overall survival or response to 5-FU in gastric
cancer. 相似文献
89.
90.
Nakada I Tabuchi T Nakachi T Shimazaki J Konishi S Katano M Ubukata H Goto Y Watanabe Y Tabuchi T 《Surgery today》2008,38(8):675-678
We analyzed the histological high-risk factors for recurrence of submucosal invasive carcinomas (pT1) of the colon and rectum after endoscopic therapy, examining pT1 cancers treated primarily by endoscopic resection within a 23-year period. We compared recurrent and nonrecurrent cancers, evaluating the following "highrisk factors" of the primary lesion: massive invasion, a surgical margin <2 mm but negativity for cancer in the cut end, poorly differentiated adenocarcinoma (PD) (G3), undifferentiated carcinoma (G4), and/or positive angiolymphatic invasion. The following histological factors were defined as predictive of a low risk: minimum invasion, a surgical margin >2 mm, well or moderately differentiated adenocarcinoma (G1, G2), and no evidence of angiolymphatic invasion. We analyzed the records of 37 patients with pT1 cancers, including 15 with high-risk factors who underwent subsequent resection. Local recurrence with or without liver metastases developed in 4 of these 15 patients. The histological type was PD in three (75%) of the four recurrent lesions. All four (100%) lesions showed a desmoplastic response (DR). Only 1 (9%) of the 11 patients without recurrence after subsequent surgery had a lesion with a small component of PD, and only three (27%) lesions showed a mild DR. We concluded that endoscopic therapy is inadequate for pT1 cancers with a histological PD component, and/or a DR in the cancer stroma. 相似文献