艾迪恒速输注对中晚期癌症患者CD3/HLA-DR与CD62P的影响

研究中药艾迪注射液对中晚期癌症患者CD3 /HLA DR、CD62 P的影响。给予中晚期癌症患者艾迪注射液 5 0mL/d ,2 4h持续恒速输注 ,14d为 1个疗程。并采用流式细胞仪检测技术 ,检测治疗前后CD3 /HLA DR、CD62 P的变化。经艾迪注射液治疗后 ,各项指标治疗前后比较 ,经统计处理 ,差异有统计学意义 ,其中CD3 /HLA DR 治疗前均值为5 3 8,治疗后均值为 9 3 1,P <0 0 5 ;CD62 P治疗前均值为 3 5 9,治疗后均值为 1 89,P <0 0 1;CD3 /HLA DR 、CD3 /HLA DR 、CD3 /HLA DR 治疗前后均值无统计学意义。CD3 /HLA DR 、CD62 P治疗后治疗组与对照组比较有统计学意义。艾迪注射液可以明显提高中晚期癌症患者CD3 /HLA DR 水平 ,并降低CD62 P ,对机体免疫功能有改善     

鼻咽癌中国1992、2008分期和国际抗癌联盟2010分期标准的比较研究

目的 比较鼻咽癌中国1992、2008分期和国际抗癌联盟(UICC)2010分期标准之间的一致性,评价它们在预测鼻咽癌放疗疗效中的价值.方法 回顾分析2000-2005年间347例无远处转移的初治鼻咽癌患者临床资料,对每例患者分别用中国1992、2008和UICC2010分期标准进行T、N和临床分期.采用Kappa法分析3种分期标准间各期病例数分布的一致性.采用Kaplan-Meier法分别计算3种分期标准的5年总生存率、局部无复发和无远处转移生存率,并用Logrank检验其差异.结果 中国2008分期和UICC 2010分期标准之间的临床分期、T和N分期的病例构成比例的一致性均优于它们各自与1992分期之间的比较,Kappa值分别为0.700、0.881和0.722.3种分期标准下各临床分期的总生存曲线比较只发现Ⅲ与Ⅳ期间的不同,其中2008分期和UICC2010分期标准下Ⅲ与Ⅳ期间的不同(χ2=4.48,P=0.034和χ2=8.88,P=0.003),而1992分期则相似(χ2=0.40,P=0.526).3种分期标准的局部无复发生存率各T1与T2和T2与T3及T3与T4期间的比较均相似(χ2=1.85、0.53、0.50,P=0.174、0.467、0.479和χ2=1.25、2.10、1.99,P=0.264、0.148、0.159及χ2=0.77、0.60、0.87,P=0.381、0.441、0.350).在3种分期标准的各期无远处转移生存率中,1992分期标准的N0与N1、N1与N2、N2与N3间均相似(χ2=3.71、3.11、2.01,P=0.054、0.078、0.156),2008分期标准的N1与N2、N2与N3间不同(χ2=10.49、5.06,P=0.001、0.024);UICC 2010分期标准中仅N1与N2间不同(χ2=7.73,P=0.005).结论 中国2008分期和UICC2010分期标准对鼻咽癌放疗疗效的预测价值相近,且均优于1992分期.

Abstract：

Objective To compare the agreement among Chinese 1992, 2008 and UICC 2010 staging systems of nasopharyngeal carcinoma (NPC) and evaluate their predictive value of radiotherapeutic prognosis.Methods 347 NPC patients without distant metastasis treated in our hospital from 2000 to 2005 were retrospectively analyzed.Every patient was categorized into T, N, and clinical stage by Chinese 1992, 2008 and UICC 2010 staging systems, respectively.Kappa value was used to evaluate the agreement among three systems.Kaplan-Meier method was used to analyze the 5-year overall survival (OS), local-free survival (LFS) and distant metastasis-free survival (DMFS), the difference between subgroup was tested by Logrank.Results The agreement of clinical stage, T and N stage between Chinese 2008 and UICC 2010 staging system was better than that of them compared to 1992 staging system, Kappa value were 0.700、0.881 and 0.722.The agreement of T stage was better than N and clinical stage among these three staging system.The difference of OS between stageⅢ and stage Ⅳ was significant in Chinese 2008 and UICC 2010 staging system (χ2=4.48,P=0.034;χ2=8.88,P=0.003), and with no different in 1992 staging system (χ2=0.40,P=0.526).There was no significant difference of LFS between T1 and T2,T2 and T3,T3 and T4 in all staging systems (χ2=1.85,0.53,0.50,P=0.174,0.467,0.479;χ2=1.25,2.10,1.99,P=0.264,0.148,0.159;χ2=0.77,0.60,0.87, P=0.381,0.441,0.350).There were no significant differencesin 1992 staging system, while there was significant differences of DMFS between N1 and N2, N2 and N3 in 2008 stage system, N1 and N2 in UICC 2010 stage system.Conclusions The predictive value of Chinese 2008 and UICC 2010 staging system for prognosis were similar, and were better than that of 1992 staging system in NPC.     

皮肤结核一例

患者,女,64岁.颈部斑块结节8年,逐渐增大.患者8年前患乳腺癌,手术切除治疗.曾诊断为毛囊炎、孢子丝菌病、乳腺癌皮肤转移等,给予相应治疗未愈.组织病理学检查:真皮内结核性肉芽肿,结节中央可见干酪样坏死,抗酸染色阳性,PAS染色阴性,真菌培养阴性,PPD(+++).抗结核治疗后皮疹明显消退.     

Function and Significance of Very Low Density Lipoprotein Receptor Subtype Ⅱ

To explore the functions of very low density lipoprotein receptor (VLDL-R) subtype Ⅱ in lipoprotein metabolism and foam cells formation, the recombinant plasmid with the two subtypes cDNA was constructed respectively, the ldl A7 cell lines were transfected and two cell lines expressing VLDL-R were obtained: one stably expressing the VLDLR with the O-linked sugar region (type Ⅰ VLDLR) and the other without the O-linked sugar region (type Ⅱ VLDLR). In the study on binding of VLDLR to their nuclein labeled natural ligands (VLDL and β-VLDL), it was found that surface binding of^125I-VLDL or ^125I-β-VLDL of ldl-A7 cells transfected with type Ⅰ VLDLR recombinant (ldl-A7-VRI) was more higher than that of ldl-A7 cells transfected with type Ⅱ VLDLR recombinant (ldl-A7 VRⅡ). After being incubated with VLDL for different time, the contents of triglyceride and total cholesterol in cells were mensurated, and the formation of foam cells and accumulation of lipid in cells was observed by oil-red O staining. The results showed that the contents of triglyceride and total cholesterol in IdI-A7-VR Ⅰ were much higher than those in ldl-A7-VR Ⅱ, and IdI-A7-VR Ⅰ could transform into foam cells notably. It was suggested that type Ⅰ VLDLR binds with relative higher affinity to VLDL and β-VLDL, and internalizes much more lipoprotein into cells. As a result, we can conclude that type Ⅰ VLDLR plays a more important role in lipoprotein metabolism and foam cells formation than type Ⅱ VLDLR。     

抗氯霉素单克隆抗体杂交瘤细胞株的制备及筛选

目的获得稳定、高效分泌抗氯霉素单克隆抗体的杂交瘤细胞株。方法以CAP-HS-BSA免疫BALB/c小鼠,用细胞融合技术筛选抗氯霉素单克隆抗体杂交瘤细胞,体内诱生腹水法大量制备单抗。捕获ELISA法测定小鼠免疫球蛋白亚型,间接ELISA法测定腹水效价;用两步硫酸铵法和G蛋白亲和层析法来纯化腹水。结果成功建立了一株分泌抗CAP单克隆抗体（McAb）的杂交瘤细胞4D10。经检测,其分泌的抗体亚类为IgG1,杂交瘤染色体数目90-110条,间接ELISA检测诱生小鼠腹水的抗体效价达1∶2.56×105,纯度达95%,该细胞连续培养生物学性状稳定。结论成功制备的抗氯霉素单克隆抗体4D10效价高、纯度高,为利用该单抗研制检测氯霉素残留试剂盒提供原材料。     

生长抑素十四肽和八肽对门脉高压症患者肝内门体分流术后门静脉血流动力学的影响

目的研究生长抑素十四肽和八肽对门脉高压症患者门静脉血流动力学的影响和外周静脉血中胰岛素样生长因子(IGF-1)、血管内皮素(ET-1)、胰高血糖素(GLU)、一氧化氮(NO)浓度变化.方法采用两交叉设计对14例门脉高压症经颈内静脉肝内门体分流术(TIPS)后患者经颈内静脉留置于门静脉内支架下方的导管,直接测静脉压,动态观察门静脉血流动力学改变,同时检测IGF-1、ET-1、GLU、NO血浆浓度.生长抑素十四肽(6 mg/d)、生长抑素八肽(0.6 mg/d)分别于TIPS术后24 h采用微量泵持续24h经周围静脉输注.用药前及用药后8、24 h进行检测.结果生长抑素十四肽和八肽用药前后门静脉压力最大差值分别为(9.4±1.0)cmH2O和(5.0±1.0)cmH2O,两者用药前后比较有非常显著性差异(P＜0.01),生长抑素十四肽与八肽两者比较有非常显著性差异(P＜0.01).IGF-1、GLU用药前后比较有显著性差异(P＜0.05).NO、ET-1用药前后比较无显著性差异(P＞0.05).结论生长抑素十四肽(6 mg/d)、生长抑素八肽(0.6 mg/d)均有降低门静脉压力的作用,但生长抑素十四肽的降压作用更为显著.两者用药后显著降低血中IGF-1、GLU浓度.其作用机制可能是通过降低血中IGF-1、GLU等激素的分泌和释放,降低肝的代谢,减少肝血流量,从而降低门静脉压力.     

肿瘤患者化疗使用PICC置管的护理

目的:探讨经外周静脉置入中心静脉导管(PICC)对肿瘤化疗患者的临床护理效果。方法:回顾性分析我科医治的60例肿瘤化疗患者,将置中心静脉导管前(静脉留置针)作为对照组,置入中心静脉导管后作为实验组,两组在穿刺点的上方给予药物敷贴等护理干预,观察两组并发症发生的情况。结果:两组静脉炎、药物外渗、导管堵管等有明显差异(P<0.05),具有可比性。结论:经外周静脉置入中心静脉导管(PICC)能够有效预防肿瘤患者化疗过程中静脉炎、药物外渗、导管堵管等并发症,能够有效保护上肢静脉,减轻患者的疼痛,提高患者的生命质量,值得临床应用推广。     

核心稳定性训练法对腰椎退行性疾病患者微创术后效果观察

目的 探讨核心稳定性训练法对经皮内镜下腰椎间盘切除术治疗腰椎退行性疾病患者术后的应用效果.方法 采用便利抽样法选取2019年10月～2019年12月在某院脊柱外科住院且行PELD治疗的60名LDD患者作为研究对象.根据接受康复护理方式的不同,将PELD术后进行核心稳定训练法护理的30名患者作为观察组,将PELD术后进行...     

动脉化静脉皮瓣成活机制的实验研究

目的 直视下观测动脉化静脉皮瓣的血液循环途径,探讨其成活机制。方法 选用15只中国大白兔30只耳朵,设计动脉化静脉皮瓣模型,应用微循环显微镜在不同时间观察皮瓣微循环的血液途径(1h、2h、4h、8h)。结果 皮瓣全部成活。皮瓣组织内毛细管管网是一个“立体”的网状结构;静脉皮瓣早期可见毛细血管床关闭,动脉血在微静脉间快速流动;随毛细血管逐渐开放,动脉血逆流进入微循环,完成循环重建。结论在引流静脉保持通畅的前提下,静脉皮瓣可借助静脉系统重建有效血液循环并保持成活。     

测试居民区住宅外鼠密度的可行性研究

目的塑料地板胶制鼠迹板测试居民区住宅外鼠密度可行性。方法在居民区住宅外分3种生境进行试验,次日观察鼠迹板阳性率、损坏率和丢失率。结果普通地面平均损坏率为49．1％,楼梯、外长廊为16．3％,楼顶、露台损坏率为10．0％。经秩和检验,普通地面与另外2种生境之间的鼠迹板损坏差别有统计学意义（U=5．72和6．37,P〈0．001）,而楼梯、外长廊与楼顶、露台之间比较鼠迹板损坏差别无统计学意义（U=0．97,P〉0．05）。结论鼠迹板适合监测鼠情,测试居民区外环境鼠密度有待研究。