Abnormal lipid metabolism down‐regulates adenosine triphosphate synthase β‐subunit protein expression in corpus cavernosum smooth muscle in vitro and in vivo

Metabolic syndrome is closely related to erectile dysfunction (ED), and hyperlipidaemia is considered a major risk factor for ED. Adenosine triphosphate (ATP) synthase is believed to play an important role in metabolic syndrome; it has been hypothesised that ATP synthase contributes to ED development. We have verified this hypothesis using primary cultured human corpus cavernosum smooth muscle (HCCSM) cells treated with excessive free fat acid (FFA) and a high‐fat diet (HFD) mouse model. Our results showed that high fatty factors could cause lipid accumulation in HCCSM cells, which could result in abnormal lipid metabolism, such as high levels of triglycerides, cholesterol and glucose in the HFD mice. There was a remarkable down‐regulation of ATP synthase and p‐Akt after in vivo and in vitro excessive FFA treatments. These results indicated that abnormal lipid metabolism could induce ATP synthase down‐regulation via the Akt phosphorylation pathway and that ATP synthase may be a target of lipotoxicity in corpus cavernosum smooth muscle cells.     

结肠CT仿真内窥镜充气方法及压力的研究

目的：探讨结肠CT仿真内窥镜较理想的充气方法和充气压力。材料和方法：8名志愿者中，男4名，女4名，最大年龄50岁，最小年龄40岁，平均44.4岁。用电脑遥控灌肠整复仪通过双腔气囊胶乳导尿管向志愿者结肠内注入气体。先行最大耐受气压CT扫描，扫描期间持续注入这个压力直到扫描结束，然后每降低7.5mmHg进行一次CT扫描，最低压力为30mmHg。结果：肛门插管用双腔气囊胶乳导尿管。CT扫描时，用持续性注入同一压力气体的方法。结肠扩张程度，在52.5mmHg，40mmHg，37.5mmHg，30mmHg 4个级别气压之间，无显著性差异。结肠充气CT扫描后，腹部不适消失时间，男女之间有显著性差异。结论：CT扫描时，持续性注入同一压力气体的方法，可以保持肠管扩张的稳定性，保证CTVE检查的顺利进行。肠管充气压力的控制，不应以患者的最大耐受为标准，应以较低的气压为妥，本研究为30mmHg。肛门插管用双腔气囊胶乳导尿管，其膨胀的气囊可以起到固定导尿管和减少导尿管与肛门间的漏气作用。CT充气扫描后，腹部不适消失时间，女性较男性为长。