复合富血小板血浆的酶处理异种骨修复兔桡骨缺损

目的:自源性的富血小板血浆可促进骨组织及软组织的修复,又不存在疾病传播及免疫排斥的可能。实验拟验证应用复合富血小板血浆的酶处理异种骨修复节段性骨缺损的可行性。方法:实验于2006-07/12在解放军昆明总医院实验动物中心完成。①实验分组:选用新西兰大耳白兔20只,体质量2.0～2.5kg,共40只前肢,随机分为复合酶组,单纯酶组,脱蛋白骨组,空白组。每组共10个标本。②实验方法:制作富血小板血浆及酶处理的异种骨并制备桡骨中段15mm的节段性骨缺损模型,将上述骨材料植入兔桡骨缺损处,其中复合酶组:植入复合富血小板血浆的酶处理异种骨;单纯酶组:植入酶处理异种骨;脱蛋白骨组:植入部分脱蛋白骨;空白组:不植入任何材料。③实验评估:分别于术后4,8,12周取材,X线片及苏木精-伊红染色分别观察骨缺损区的新骨形成情况。同时对各组骨密度进行测定。结果:纳入新西兰大耳白兔20只,均进入结果分析,所有手术无术后感染,无动物死亡,植入骨无脱落。①所有动物麻醉苏醒后均恢复进食,2周伤口愈合,未出现感染及渗液等。富血小板血浆中的血小板含量均为全血的4倍以上。②在同一时间点,酶处理后异种骨与部分脱蛋白骨在成骨能力方面差异无显著性意义,而复合富血小板血浆的酶处理异种骨在8周时骨缺损部分修复;12周时完全修复。空白组骨缺损未修复。③8,12周时复合酶组骨密度较单纯酶组、脱蛋白骨组高,差异有显著性意义(P<0.05);单纯酶组与脱蛋白骨组比较无明显差异(P>0.05)。结论:经酶处理后异种骨可用于节段性骨缺损的修复,复合富血小板血浆后有明显加速骨愈合的作用。     

尼贝沙坦联合胺碘酮治疗阵发性心房颤动的临床观察

目的：观察尼贝沙坦联合胺碘酮治疗阵发性心房颤动的临床效果与安全性。方法：将60例阵发性心房颤动患者平分为治疗组与对照组各30例,在各种常规治疗的基础上,对照组加用尼贝沙坦治疗,治疗组加用贝沙坦联合胺碘酮治疗。结果：经过治疗后,两组都无死亡患者,治疗组总有效率为90.0%,对照组总有效率为70.0%,治疗组的临床疗效明显好于对照组（P〈O.05）。而对照组的脑栓塞和脑出血发生率都明显高于治疗组（P〈0.05）。结论：尼贝沙坦联合胺碘酮治疗阵发性心房颤动的临床效果好,安全性好,值得推广应用。     

汝康胶囊毒理学安全性实验研究

检测汝康胶囊作为保健食品的安全性.方法:依据卫生部<保健食品检验与评价技术规范>[1],通过小鼠经口急性毒性试验、致突变试验,大鼠30天喂养试验对汝康胶囊进行检测.结果:小鼠经口LD50＞21.5g/kg·bw,属无毒级物质;致突变试验结果为阴性;大鼠30天喂养试验表明各剂量组体重增长、食物利用率、脏体比、血液学及血清...     

血清胸苷激酶1对乳腺癌辅助诊断及术后化疗效果评估的意义

目的:探讨血清胸苷激酶1(thymidine kinase 1,TK1)检测对乳腺癌辅助诊断及术后化疗效果评估的意义?方法:应用免疫印迹—增强化学发光法(ECL)检测48例乳腺肿瘤患者的血清TK1水平,并对其中随访1~3个月的14例术后化疗的血清TK1水平进行检测?结果:10例完成4~6个周期化疗后的血清TK1降低并接近正常人水平,4例血清TK1未降反升?随访1年后,有1例发生骨转移?结论:血清TK1水平反映细胞增殖,与病灶大小有一定相关性,连续跟踪结果有利于对乳腺癌治疗效果的辅助诊断,对临床监视肿瘤疗效具有临床意义?     

再论强化举证责任倒置意识

目的论述病案在举证倒置法律中的重要性。方法通过比较2002年以前患办提供的证据与2002年4月1日以后的病案证据指明强化病案管理的重要意义。结果强化病案管理对举证责任倒置具有重要价值。结论强化病案管理,是举证责任倒置,减少医疗纠纷,加强医务人员自身保护意义的主要前提。     

丹红注射液治疗182例冠心病心绞痛的疗效观察

目的探讨丹红注射液对冠心病心绞痛患者的临床疗效价值。方法随机按治疗组92例和对照组90例把182例冠心病心绞痛患者分为两组,对照组给予常规静脉滴注葡萄糖、氯化钾、普通胰岛素500mL,治疗组给予5%葡萄糖液500mL加丹红注射液20mg静脉滴注,两组均为每日1次,14日为1个疗程,比较两组的疗效和不良反应。结果治疗组用药后与用药前相比,丹红注射液对心绞痛的疗效总有效率达92%,试验过程中未出现不良反应。结论丹红注射液是治疗冠心病心绞痛理想的新型中药制剂,具有较好的疗效和安全性。     

Synthesis and Discovery of Novel Pyrazole Carboxamide Derivatives as Potential Osteogenesis Inducers

A series of novel N‐aryl‐3‐aryl‐1‐arylmethyl‐1H‐pyrazole‐5‐carboxamide derivatives 4a – l was synthesized by the reaction of 3‐aryl‐1‐arylmethyl‐1H‐pyrazole‐5‐carbonyl chloride with substituted aniline in good to excellent yields. Structures of the compounds were determined by IR, ¹H NMR, and HR‐MS spectroscopy. The molecular structure was confirmed by the X‐ray crystal analysis of one compound ( 4j ) that was prone to crystallization. These compounds were used to induce mouse osteoblast precursors MC3T3‐E1 into osteoblasts and the induction was assessed by alkaline phosphatase (ALP) activity and the gene expression of bone sialoprotein (BSP). The results showed that the compounds 4a – d , 4g , 4h , and 4k could increase the ALP activity in comparison with the negative control group and compound 4h was the most effective one which could induce osteogenesis. Furthermore, mRNA expression of BSP which is a marker of osteogenesis was up‐regulated by the compound 4h .     

Invasive Breast Carcinoma Arising in Microglandular Adenosis: A Case Report and Review of the Literature

Abstract:  Microglandular adenosis of the breast is an extremely rare, benign lesion. Carcinoma arising in microglandular adenosis has been reported in up to 27% of patients with microglandular adenosis. We reported a case of invasive carcinoma arising in microglandular adenosis. A lumpectomy was performed on a 42-year-old female because of a mass in the right breast. Grossly, the lesion was ill-defined with thickened areas. Histologically, this case was consistent with carcinoma arising in microglandular adenosis and showed clear transition from microglandular adenosis through atypical microglandular adenosis to in situ and invasive carcinoma. All the epithelial cells were positive for cytokeratin7, S-100 protein, but negative for estrogen receptor, progesterone receptor, and Her2/Neu. No myoepithelial cells were demonstrable with immunohistochemical staining for smooth muscle actin, muscle-specific antigen or P63. Periodic acid-Schiff staining showed the presence of a basement membrane in microglandular adenosis, atypical microglandular adenosis, in situ carcinoma and the absence in invasive carcinoma. To the best of our knowledge, this is the first reported case of carcinoma arising in microglandular adenosis in China.     

Wilson disease： Identification of two novel mutations and clinical correlation in Eastern Chinese patients

AIM To study mutations in the P-type ATPase (ATP7B)gene responsible for Wilson disease (WD) in the Eastern Chinese population, and the possible correlation of specific mutations with clinical characteristics.METHODS Mutations of the ATP7B gene were sought by means of direct sequencing in 50 Eastern Chinese WD patients of Han ethnic origin.RESULTS Two novel mutations, Asp96Gly and Asp196Glu, were first identified. We also compared the characterization of mutations in ATP7B with the clinical findings, and a significant correlation with hepatic manifestations between patients carrying the Arg778Leu mutation and those without was found.CONCLUSION Gene sequencing analysis was shown to have a high detection rate and accuracy. It may become the first priority in screening of WD patients.