槐耳清膏体外逆转人乳腺癌细胞MCF-7耐三苯氧胺的研究

目的 探讨槐耳清膏体外逆转人乳腺癌细胞MCF-7耐三苯氧胺(TAM)的作用及其机制.方法 使用噻唑蓝(MTT)比色法测定不同药物处理对耐TAM的乳腺癌细胞株MCF-7/R的抑制率,流式细胞仪检测不同处理后MCF-7/R的凋亡情况和细胞周期变化.Western blot检测MCF-7/R不同处理后各组Phospho-P44/42MAPK(ERK1/2)及P44/42MAPK(ERK1/2)表达的差异.结果 MTT实验结果显示MCF-7/R的联合用药组抑制率(45.99±6.06)%和单药组抑制率(40.20±5.54)%分别与TAM对照组比较,差异有统计学意义(P＜0.05);流式细胞检测结果显示与TAM对照组比较,MCF-7/R的联合用药组和单药组G1期峰前出现显著的凋亡峰,且联合用药组的凋亡率(20.03%)与单药组的凋亡率(11.06%)与TAM对照组(2.15%)比较作用显著;Western blot检测蛋白表达经灰度分析显示,药物作用12 h后Phospho-P44/42MAPK(ERK1/2)蛋白在联合用药组的表达(0.3153±0.0179)和单药组的表达(0.5362±0.0030)分别与TAM对照组(0.9752±0.0034)比较均有下降,差异有统计学意义(P＜0.05),且联合组与单药组比较,差异有统计学意义(P＜0.05).结论 槐耳清膏具有体外逆转MCF-7/R细胞对TAM的耐药作用,逆转机制可能与下调MAPK通路磷酸化蛋白ERK1/2的表达水平相关,提示槐耳清膏是一种有意义的耐药逆转剂.     

The management of epilepsy in pregnancy

While most women with epilepsy can expect a normal pregnancy outcome, epilepsy remains a significant contributor to both maternal and perinatal morbidity. Pre-pregnancy planning must address reliable contraception and optimisation of antiepileptic drug (AED) regimens to minimise teratogenic risk while maintaining seizure control. The most recent data from the AED registries regarding malformations is presented in this review, as is the limited data on the newer AEDs and studies linking neurocognitive outcomes to AED exposure. During pregnancy, important considerations include; therapeutic drug monitoring, surveillance for obstetric complications and vigilance for seizures during the intrapartum and postpartum period.     

Mid-gut volvulus and mesenteric vessel thrombosis in pregnancy: case report and literature review

Objective  

To compare the maternal bone mineral density (BMD) changes in gestational hypertensive and normotensive pregnancies using quantitative ultrasound.     

Feasibility of using interpolated contours of targets and organs at risk in intensity‐modulated radiation therapy treatment planning for advanced‐stage nasopharyngeal carcinoma

To assess the dosimetric effect of using interpolated contours in planning intensity‐modulated radiation therapy (IMRT) for advanced T‐stage nasopharyngeal carcinoma. The present study focused on T3–T4 tumours where the proximity of targets to neurological organs poses a stringent test on the feasibility of such an approach. Contours of targets and organs were delineated on CT images of 2.5‐mm interval and a reference IMRT plan was generated. An investigative (INV) IMRT plan was then generated with the same planning protocol, but based on interpolated contours that replaced deleted contours on alternate slices. The reference and INV plans were compared. Regarding target coverage, all targets in the INV plans met the acceptance criteria except for the PTV in one case. Regarding organs, the mean dose to 1% volume of the brainstem and spinal cord in the INV plans were kept below their dose limits. No significant differences in the mean doses to others organs were found. Satisfactory target coverage and protection of critical organs to a degree similar to full‐scale contouring could be achieved with use of interpolated contours. The saving in manpower time for contouring is expected to significantly improve the throughput of the IMRT planning process.     

HPLC法测定宽胸舒气化滞丸中咖啡酸的含量

目的：建立宽胸舒气化滞丸中咖啡酸的含量测定方法。方法：采用HPLC法测定，色谱柱为十八烷基硅烷键合硅胶填充柱（4．6mm×250mm，5μm），柱温40℃，流动相为乙腈-0．04％磷酸溶液（11：89），流速为1．0mL·min^-1，检测波长为325nm。结果：咖啡酸在0．0516μg-1．032μg（r=0．9999）范围内呈良好的线性关系，平均回收率为100．57％，RSD为1．75％（n=6）。结论：实验表明，该方法准确，灵敏度高，重现性好。     

华南汉族健康者凝血因子VII R353Q基因型的分布

目的观察凝血因子VII(coagulationf actor VII,FVII)R353Q基因多态性在华南汉族健康者人群中的分布。方法提取华南汉族60名正常健康者基因组DNA,应用多聚酶链反应(PCR)技术和限制性内切酶片段长度多态性技术检测上述人群的FVII R353Q基因型。结果华南汉族人群FVII基因R353Q具3种基因型(RR,RQ,QQ),FVII等位基因R、Q基因频率在人群中分别为90.9%,9.1%。基因型频率符合Hardy-Weinberg平衡定律。结论华南汉族健康人群凝血因子VII基因R353Q多态具3种基因型(RR,RQ,QQ),R等位基因携带频率明显高于Q等位基因。     

低剂量紫杉醇联合吉西他宾治疗转移性乳腺癌的临床疗效分析

目的：评估低剂量紫杉醇（paclitaxel,PTX）和吉西他滨（gemcitabine,GEM）3周联合化疗方案在既往接受过蒽环类药物治疗的转移性乳腺癌（metastatic breast cancer,MBC）患者的临床疗效。方法：32例既往用过蒽环类药物治疗的MBC患者,PTX120mg/m^2,输注3h,d1,GEM900mg/m^2,输注30min,d1和d8。每3周重复,最多接受6个周期治疗。所有患者均评估不良反应,对至少用过2个周期的患者评估疗效。结果：32例患者,共完成156个周期化疗,中位化疗周期数为5个周期。完全缓解4例（12.5%）,部分缓解15例（46.9%）,有效率59.4%（95%可信区间：42.4%～76.4%）,稳定8例（25%）,进展5例（15.6%）。有效率在不同年龄组患者、ER（＋）和ER（-）患者、浸润性导管癌和浸润性小叶癌患者之间差异均无统计学意义。平均随访20.2个月,有效者中位疾病进展时间为10.0个月,中位总生存时间为20.0个月。至随访结束,9例患者仍无进展存活。结论：低剂量PTX和GEM3周联合化疗方案对既往接受过蒽环类药物治疗的MBC患者是安全有效的,血液学和非血液学毒性都可耐受。     

血管紧张素原基因与黎族原发性高血压并脑梗死相关性

目的 研究血管紧张素原（AGT）基因四个位点的单核苷酸多态及其构成的单倍型与海南黎族原发性高血压并脑梗死的相关性.方法 选择海南黎族人群为研究对象,利用PCR-直接测序法,在100例原发性高血压并脑梗死患者和100例健康对照者中,对AGT基因启动子区域的G-152A,A-20C,C-18T,A-6G多态进行基因分型.结果原发性高血压并脑梗死组与健康对照组相比,C-18T多态的基因型和等位基因频率分布差异均有显著性（P=0.002,P=0.002）.A-6G多态的G等位基因频率在原发性高血压并脑梗死组显著高于对照组（P=0.016）.单倍型分析提示由-20C和-6G构成的H6单倍型在原发性高血压并脑梗死组中明显增加,与对照组相比有显著性差异（P=0.003）.结论 在海南黎族人群中,A-20C,C-18T,A-6G多态可能是原发性高血压并脑梗死发病的遗传危险因素.     

骨髓活组织检查在慢性粒-单核细胞白血病中的诊断意义

目的评价骨髓活检在慢性粒-单核细胞白血病（CMML）中的诊断价值。方法观察42例CMML病人的骨髓涂片与骨髓活检切片，并做出比较和分析。结果骨髓涂片与切片相互结合起到相互补充的作用。结论骨髓切片与涂片结合起来更能提高CMML的检出率。