Blood group substances as differentiation markers in human dento-gingival epithelium

The level of cellular differentiation of human oral, sulcular, and junctional epithelium was compared by immunohistochemical analysis of cell membrane-associated blood group-specific carbohydrates. Identification of the blood group A-specific carbohydrate and its two immediate precursor substances, type 2 chain H and N-acetyllactosamine, was accomplished by an indirect immunofluorescence technique. Murine monoclonal antibodies reacting specifically with the antigenic determinants of the blood group substances were used as markers. The blood group A substance, indicating the highest level of cellular differentiation, was demonstrated on the cells in the upper layers of the oral epithelium. In the sulcular epithelium, the A substance was present on a few cells only, while type 2 chain H was observed frequently. This indicates an intermediate differentiation level of sulcular epithelium. The type 2 chain H precursor, N-acetyllactosamine, the indicator of the lowest level of cell differentiation among the tested substances, was the only blood group substance detected on the junctional epithelial cells and on the basal cells of the sulcular and oral epithelium. Based upon previous studies of cell renewal and differentiation in oral epithelium, the present results indicate that the variations in distribution of the different blood group substances correspond with the regional rates of cell division and the levels of cellular differentiation. The findings also suggest that the cells in the junctional epithelium differentiate to a level similar to that of basal cells in the oral epithelium.     

Dispensing and weighing apparatus for alginate impression material

Constant proportions in mixing of alginate impression material are difficult to achieve, especially in a teaching department, owing to variability in powder measurement with the conventional scoop method. Apparatus to facilitate powder measurement by weighing is described, and its accuracy assessed.     

The effect of early heating on the expansion of a phosphate bonded investment

A bstract — The setting expansion of a phosphate bonded investment continued for more than six hours at room temperature. Specimens heated to about 100°C within six hours of mixing expanded rapidly, the degree of expansion increasing with reduction of time between mixing and heating and with increase in colloidal silica concentration of the liquid. The rapid expansion on heating was considered to be a combination of thermal and accelerated setting expansion. Time between mixing and heating could be an important variable with regard to obtaining optimal expansion.