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61.
S. Martin J. Kardorf B. Schulte E. F. Lampeter F. A. Gries I. Melchers R. Wagner J. Bertrams B. O. Roep A. Pfützner M. Pietropaolo H. Kolb 《Diabetologia》1995,38(3):351-355
Summary Islet cell antigen (ICA) 69 is a newly-recognized islet cell antigen to which autoantibodies have been observed in prediabetic relatives of patients with insulin-dependent-diabetes mellitus (IDDM). Here we extend the earlier analysis of ICA 69 antibodies to patients with recent-onset IDDM and to patients with other immune-mediated diseases. ICA 69 antibodies were determined by Western blot using an affinity purified recombinant fusion protein of ICA 69 and maltose binding protein. ICA 69 antibody quantities were determined as titres using a titration curve of a standard serum as reference. Mean logarithmic ICA 69 antibody titres were 3.4 (±1.4) in 99 patients with acute IDDM compared to 2.8 (±0.9) in 49 healthy blood donors (p<0.001). A higher mean ICA 69 antibody titre of 4.1 (±0.8) was observed in 16 patients with rheumatoid arthritis in comparison to acute IDDM (p<0.01) and healthy control subjects (p<0.001). The percentage of sera with ICA 69 antibody titres above the 2 SD level of normal subjects was 21% in IDDM, 31% in rheumatoid arthritis and 6% in healthy blood donors. None of the patients with autoimmune thyroid disease (n=20), inflammatory bowel disease (n=9) or multiple sclerosis (n=7) had elevated ICA 69 antibodies. In IDDM, presence of ICA 69 antibodies persisted and the titre remained the same over 18 months of follow-up. The relationship of ICA 69 antibodies to islet cell antibodies (ICA) or insulin autoantibodies (IAA) was tested. The production of ICA 69 antibodies was not associated in diabetic patients with the presence of any of the two other autoantibodies. In conclusion, this study describes ICA 69 antibodies in acute IDDM and finds them to be independent of other islet autoantibodies. In addition ICA 69 is a target of humoural autoimmunity not only in IDDM but also in rheumatoid arthritis.Abbreviations IDDM
Insulin-dependent diabetes mellitus
- ICA
islet cell antibodies
- IAA
insulin autoantibodies
- RA
rheumatoid arthritis
- RF
rheumatoid factor
- GAD 65
glutamic acid decarboxylase
- SMS
stiff-man syndrome 相似文献
62.
Die periphere arterielle Verschlusskrankheit wird leider auch heute noch zu selten diagnostiziert und in ihrer prognostischen
Bedeutung als Markerkrankheit für eine hohe Mortalit?t der betroffenen Patienten untersch?tzt. AVK-Patienten haben ein 4-
bis 6fach erh?htes kardiovaskul?res Risiko. Das vorrangige Problem von Claudicatio-Patienten ist deshalb nicht nur die Beeintr?chtigung
ihrer individuellen Gehleistung, sondern das hohe Risiko für kardiovaskul?re Komplikationen. Die Patienten sterben nicht an
ihrer AVK sondern am Herzinfarkt (70%) oder Schlaganfall (5%) und weiteren atherothrombotischen vaskul?ren Komplikationen.
Wichtig ist ein aggressives Risikofaktorenmanagement. Insbesondere der Risikofaktor Rauchen muss mit Nachdruck bek?mpft werden.
Ein kontrolliertes Gehtraining und alternativ oder unterstützend vasoaktive Medikamente sind, wenn sie gezielt und kritisch
zum Einsatz kommen dem Wohl des Patienten f?rderlich.
In jedem Fall ist die frühzeitige Gabe eines Thrombozytenfunktionshemmers sowohl für die Behandlung im Stadium der Claudicatio
intermittens als auch bei der kritischen Extremit?tenisch?mie angezeigt. Bei Patienten, bei denen keine operativen oder interventionellen
Ma?nahmen durchgeführt werden k?nnen oder bei denen es zu keiner Lumener?ffnung kommt, ist eine medikament?se Behandlung mit
Prostanoiden indiziert. 相似文献
63.
Berit Schulte Gabriele Bierbaum Konstanze Pohl Christiane Goerke Christiane Wolz 《Journal of clinical microbiology》2013,51(1):212-216
Since 1995, a methicillin-resistant Staphylococcus aureus (MRSA) clone has spread in southern Germany. The strain was assigned to the Rhine-Hesse pulsed-field gel electrophoresis (PFGE) type by the staphylococcal reference center and was highly similar to epidemic clones known to belong to clonal complex 5 (CC5; USA100) based on multilocus sequence typing (MLST). Here we analyzed a defined collection of strains assigned to the Rhine-Hesse/USA100 PFGE type. Using sequence-based typing methods (MLST, spa), the isolates were divided into two distinct clusters, ST5 and its single-locus variant ST225. These two lineages are not distinguishable by PFGE or phage typing. Most of the ST5 isolates were derived from patients and volunteers from the Tübingen area in southwest Germany, whereas the ST225 isolates were mostly from other locations in Germany. The locally restricted ST5 isolates were shown to contain different SSCmec islands and exhibited different antibiotic resistance profiles. In contrast, the ST225 isolates form a highly homogenous group and are emerging all over Germany. The two lineages are clearly distinguishable by their phage content and spa type: ST5 strains from Tübingen are characterized by a Sa7int phage that carries the virulence gene sak, which codes for staphylokinase, and ST225 isolates are characterized by a Sa1int phage. In conclusion, based on sequence typing and phage content, CC5 strains can be subdivided into two distinct lineages with different epidemicities. 相似文献
64.
65.
Experimental validation of the hyperpolarized 129Xe chemical shift saturation recovery technique in healthy volunteers and subjects with interstitial lung disease 下载免费PDF全文
66.
Gitta Bleeker Berthe L. van Eck-Smit Koos H. Zwinderman Rogier Versteeg Max M. van Noesel Boen L. Kam Gertjan J. Kaspers Annelies van Schie Susan G. Kreissman Gregory Yanik Barbara Hero Matthias Schmidt Geneviève Laureys Bieke Lambert Ingrid Øra Johannes H. Schulte Huib N. Caron Godelieve A. Tytgat 《European journal of nuclear medicine and molecular imaging》2015,42(2):222-230
67.
68.
Thrombopoietin (TPO) or MpI ligand is known to stimulate megakaryocyte (MK) proliferation and differentiation. To identify the earliest human hematopoietic cells on which TPO acts, we cultured single CD34+Thy- 1+Lin- adult bone marrow cells in the presence of TPO alone, with TPO and interleukin-3 (IL-3), or with TPO and c-kit ligand (KL) in the presence of a murine stromal cell line (Sys1). Two distinct growth morphologies were observed: expansion of up to 200 blast cells with subsequent differentiation to large refractile CD41b+ MKs within 3 weeks or expansion to 200-10,000 blast cells, up to 25% of which expressed CD34. The latter blast cell expansions occurred over a 3- to 6-week period without obvious MK differentiation. Morphological staining, analysis of surface marker expression, and colony formation analysis revealed that these populations consisted predominantly of cells committed to the myelomonocytic lineage. The addition of IL-3 to TPO-containing cultures increased the extent of proliferation of single cells, whereas addition of KL increased the percentage of CD34+ cells among the expanding cell populations. Production of multiple colony- forming unit-MK from single CD34+Thy-1+Lin- cells in the presence of TPO was also demonstrated. In limiting dilution assays of CD34+Lin- cells, TPO was found to increase the size and frequency of cobblestone areas at 4 weeks in stromal cultures in the presence of leukemia inhibitory factor and IL-6. In stroma-free cultures, TPO activated a quiescent CD34+Lin-Rhodamine 123lo subset of primitive hematopoietic progenitor cells into cycle, without loss of CD34 expression. These data demonstrate that TPO acts directly on and supports division of cells more primitive than those committed to the MK lineage. 相似文献
69.
70.
Ali Abbasi Eva Corpeleijn Ron T. Gansevoort Rijk O. B. Gans Joachim Struck Janin Schulte Hans L. Hillege Pim van der Harst Ronald P. Stolk Gerjan Navis Stephan J. L. Bakker 《Diabetologia》2014,57(9):1842-1849