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41.
C. Haupt U. Ancker M. Müller H. D. Herrmann F. J. Schulte 《European journal of pediatrics》1996,155(3):230-236
There is only sparse information about the development of children after successful treatment for intracranial germ-cell tumours. Between January 1981 and June 1992, 26 children with intracranial germ-cell tumours were treated in the University Hospital Hamburg-Eppendorf. We report on treatment results, long standing residuals and the quality of life of these patients. The long-term event-free survival was 88% for the germinomas and 43% for the malignant teratomas. Of the patients 58% had no relevant functional neurological deficits and 37% had mild impairment. Only 1 patient with metastatic disease was severely handicapped. Six patients showed neuro-endocrine dysfunction. All of them had suprasellar/hypothalamic lesions and all received successful substitution therapy. As to neuropsychological functions, 53% of the patients had no or only mild disturbances. The most affected function was speed of information processing. Of the children 69% were able to proceed with their education at the same level as before therapy. The overall self-assessment revealed good results in 75% of the patients.Conclusion After surgical removal and radiation therapy long-term survival of intracranial germinomas amounts to 88%. Despite craniospinal axis radiation severe residuals are rare and a good quality of life is common. In malignant teratomas treatment regimens including chemotherapy are much less successful. 相似文献
42.
The essential oil content of various populations of MELISSA OFFICINALIS L. (Lamiaceae), cultivated under Mediterranean climatic conditions (Ebro-Delta, Spain) has been investigated during five years of selection and improvement of the genetic plant material. Starting with an essential oil content of 0.2-0.3%, a content of more than 0.5% was obtained as a result of genetic improvement. A weak negative correlation between the content of essential oil and phenotypical growth parameters such as the number of branches per plant and height was observed. No correlation between biomass production per plant and essential oil content could be found. By the method of hybridization, M. OFFICINALIS synthetics with a high yield of biomass and essential oil content were selected. 相似文献
43.
M A Schulte 《Dermatology nursing / Dermatology Nurses' Association》1991,3(5):335-339
As the body's largest organ, the skin functions as the most important human communication organ, possessing communicative aspects of caring and healing of self and others. The program "Self-Care Activating Support" for psoriasis and other chronic skin disease is presented as an instrument for promoting health by dermatology nurses. 相似文献
44.
Growth Regulation of Thyroid and Thyroid Tumors in Humans 总被引:1,自引:0,他引:1
In a study of growth regulation of the human thyroid gland and thyroid tumors we investigated the impact of iodine and that
of the thyroid-specific growth-stimulating hormone TSH. Further studies included locally active growth factors such as the
epidermal growth factor, insulin-like growth factor, and tissue transforming growth factors alpha and beta. In addition to
studies of growth regulation by the various growth factors in mostly normal thyrocytes, the impact of tumor-specific mutations
in oncogenes and tumor-suppressor genes was investigated. The results demonstrated distinct changes in tissue specificity
and sensitivity to external stimuli. This rather complex view on thyrocyte growth regulation may be confusing, but it describes
the biologic reality more precisely. Increased knowledge of the regulatory processes may lead to the development of new tumor-
and patient-specific therapeutic approaches, especially for preventing benign goiter recurrence and for treating follicular
and papillary thyroid cancers. 相似文献
45.
n = 11, 52%), renovascular stenosis (n= 9, 43%) with concurrent renovascular hypertension (n= 5, 24%), and angina abdominalis (n= 7, 33%). Most patients had multiorgan vascular disease such as iliofemoral arterial occlusive disease (n= 14, 66%), coronary artery obstruction (n= 8, 38%), or obstruction of the carotid artery (n= 6, 28%). Risk factors did not differ between coral reef patients and those with other occlusive vascular diseases. All patients
were treated through vascular operations, including open thromboendarterectomy of the suprarenal (n= 9, 43%), infrarenal (n= 4, 19%), or supra- and infrarenal aorta (n= 8, 38%), and thromboendarterectomy of the following vessels: celiac artery (n= 7, 33%), superior mesenteric artery (n= 12, 57%), inferior mesenteric artery (n= 3, 14%), unilateral renal artery (n= 3, 14%), or bilateral renal artery (n= 9, 43%). Bypass reconstructions were performed in 39% (n= 8). A thoracoabdominal approach was used in 14 patients (67%) and a median laparotomy in 7 (33%). Our results show that
coral reef aorta is not confined to either gender. It appears most frequently in the context of general atherosclerotic disease
and patients benefit from timely diagnosis and operation before onset of severe, life-threatening visceral and renal complications. 相似文献
46.
Comparison of total alkaline phosphatase and three assays for bone-specific alkaline phosphatase in childhood and adolescence 总被引:2,自引:0,他引:2
F Rauch B Middelmann M Cagnoli KM Keller E Schönau 《Acta paediatrica (Oslo, Norway : 1992)》1997,86(6):583-587
We compared serum levels of total alkaline phosphatase (TAP) and bone-specific alkaline phosphatase (BAP) as determined by three different assays (lectin affinity electrophoresis, immunoradiometric assay, enzyme-linked immunosorbent assay) in subjects aged 5–20 years suffering from X-linked hypophosphatemic rickets ( n = 14), chronic renal failure ( n = 10) and chronic cholestatic liver disease ( n = 16). Results were compared to controls of the same age and were expressed as standard deviation scores (SDS). TAP correlated significantly with BAP ( r > 0.9 for each assay; p <0.001) in controls. In children with cholestatic diseases, TAP (median SDS + 2.0) was elevated, but BAP, as measured by the electrophoretic assay, was within the reference range for most patients (median SDS: -0.4; p = 0.003 for the difference between the median SDS of TAP and BAP). In contrast, results for BAP as determined by the two immunoassays were not significantly different from TAP in any of the three patient groups ( p > 0.05 in each group for both assays). In this study, the two immunoassays did not have a detectable advantage over lectin affinity electrophoresis in the determination of BAP. 相似文献
47.
Isolation and characterization of propagable cell lines (HUNC) from the androgen-sensitive Dunning R3327H rat prostatic adenocarcinoma 总被引:1,自引:0,他引:1
Presnell SC; Borchert KM; Glover WJ; Gregory CW; Mohler JL; Smith GJ 《Carcinogenesis》1998,19(4):585-590
The Dunning H rat prostate tumor (R3327H) is a widely used experimental
model of human prostatic adenocarcinoma (CaP). The Dunning H tumor has been
characterized as androgen-sensitive, androgen-receptor (AR) positive,
prostate-specific antigen and prostatic acid phosphatase (PAP) positive. To
date, the tumor has been maintained by serial passage in vivo because of
the lack of an in vitro cell line that retains the characteristics of the
in vivo tumor. The objective of the present study was to establish a
propagable cell line from R3327H adenocarcinoma that maintained androgen
sensitivity and expression of AR, PSA and PAP. Tissue harvested from an in
vivo R3327H tumor was dissociated with collagenase and placed into
Richter's improved media (with supplements). A cytokeratin-positive
epithelial cell line (HUNC- E) and a vimentin-positive stromal cell line
(HUNC-S) were generated from the primary culture, subcultured continuously
for >300 days, and passaged >50 times. Survival of the HUNC-E cell
line in vitro depended on several media supplements, including
nicotinamide, insulin, transferrin, selenium and epidermal growth factor
(EGF). HUNC-E cells expressed AR and produced PSA and PAP throughout the
culture period, as confirmed by immunocytochemistry and Western blot
analyses. Addition of 14 nM testosterone (T) or dihydrotestosterone (DHT)
to HUNC-E cells, stimulated DNA synthesis as well as anchorage-independent
growth and PSA production, which demonstrated the androgen-sensitive nature
of the cells in vitro. When HUNC-E and HUNC-S cells were combined in a 3:1
ratio and introduced subcutaneously into syngeneic male hosts, tumors
formed in 2/3 animals with an average latency of 7 months. RT-PCR and
immunocytochemical characterization of the HUNC cell lines revealed that
the cells expressed several growth factors and their cognate receptors,
including HGF, TGF-alpha and the TGF-betas, indicating the establishment of
potential autocrine loops in the neoplastic cells. The HUNC-E and HUNC-S
CaP cell lines, which retain the characteristics of the epithelial and
stromal components of the in vivo R3327H tumor, will allow a more thorough
and informative molecular and biological analysis of prostatic
adenocarcinoma.
相似文献
48.
Carcinogenicity and DNA adduct formation observed in ACI rats after long-term treatment with madder root, Rubia tinctorum L 总被引:1,自引:0,他引:1
Madder root, Rubia tinctorum L., is a traditional herbal medicine used
against kidney stones. Recently we reported that lucidin, a
hydroxyanthraquinone derivative present in this plant, is mutagenic in
bacteria and mammalian cells. We also demonstrated the formation of DNA
adducts in tissue culture and mice after treatment with this compound. To
elucidate the possible carcinogenicity of madder root, three groups of male
and female ACI rats received either a normal diet or a diet supplemented
with 1 or 10% drug for a total period of 780 days. Weight gain and
morbidity were not different among the three groups. Non- neoplastic
lesions related to the treatment were evident in the liver and kidneys of
both sexes. Moreover, dose-dependent increases in benign and malignant
tumour formation were observed in the liver and kidneys of treated animals.
32P-post-labelling analysis showed an increase in the overall level of DNA
adducts observed in the liver, kidney and colon of rats treated with 10%
madder root in the diet for 2 weeks. HPLC analysis of 32P-labelled DNA
adducts revealed a peak co-migrating with an adduct obtained after in vitro
treatment of deoxyguanosine-3'- phosphate with lucidin. These observations
suggest that the use of madder root for medicinal purposes is associated
with a carcinogenic risk.
相似文献
49.
50.
Cytochrome P450 (P450) monooxygenases play a role in target tissue metabolic activation of xenobiotics and/or endogenous compounds, such as vasoactive molecules or hormones. Indeed, tissue-specific metabolism of steroids is important in a variety of organs, including thymus, and may alter tissue-specific functions. Steroids have been shown to regulate thymus growth and function, but surprisingly little is known about expression of the responsible enzyme systems in thymus tissue, nor is the thymus-specific biotransformation of testosterone known. We therefore investigated gene and protein expression, total protein content, and enzyme activity of major P450 isoforms and other key steroid-metabolizing enzymes in thymus tissue of adult and fetal rats. We detected 6 beta-hydroxytestosterone (HT), 7 alpha-HT, 16 alpha-HT, 2 alpha-HT, and androstenedione to be major testosterone metabolites in the adult thymus. The high production of 7 alpha-HT and 16 alpha-HT correlated well with the gene and protein expression of CYP2A1/2 and CYP2B1/2 in thymus of adult animals. When compared with fetal thymic tissue, CYP2A1/2, 17beta-hydroxysteroid dehydrogenase isoform 1 (17 beta-HSDH1) and the androgen receptor were 8-, 3-, and 3-fold more highly expressed in adult rats, whereas 17 beta-HSDH2, 17 beta-HSDH3, and 5 alpha-reductase were reduced to 12%, 0%, and 32% of those in fetal thymus. In conclusion, we demonstrated that rat thymus expresses a variety of cytochrome P450 monooxygenases and other steroid-metabolizing enzymes, and it successfully metabolizes testosterone. Changes of the underlying steroid-metabolizing enzyme systems may aid in understanding the role of androgens in altering biological functions of the thymus. 相似文献