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31.
Expression of differential nitric oxide synthase isoforms in human normal gastric mucosa and gastric cancer tissue 总被引:10,自引:2,他引:10
The present study investigated the expression and distribution of three
isoforms of nitric oxide synthase (NOS) in different anatomical regions of
the human stomach and in gastric neoplastic tissues by immunohistochemistry
using specific antibodies. Intracellular localization of individual
isoenzymes of NOS was detected in normal gastric mucosa. Gastric cancer
tissues had a marked reduction of all three NOS isoforms expression. The
expression of the endothelial NOS, neuronal NOS and inducible NOS in the
tumor tissue was significantly lower than in normal gastric mucosa (P =
0.01, P = 0.02, P < 0.01, respectively). In the tumor tissue the
expression of inducible NOS was significantly lower than the expression of
both constitutive forms of NOS (P < 0.01). There was a tendency to
higher expression of both constitutive forms of NOS in earlier stages T2 of
the tumor compared to advanced T4 tumor. In contrast, the expression of
inducible NOS was higher than in the advanced T4 tumor than in the earlier
stages T2 of the tumor. The mapping of the expression of endothelial NOS,
neuronal NOS and inducible NOS in human stomach showed higher expression of
NOS isoforms in the distal third than in the proximal third of the stomach
(P = 0.03, P = 0.04, P = 0.01, respectively). We conclude that there is
greater expression of NOS in the stomach corpus and in antrum than in the
proximal third of the normal human stomach mirroring the anatomical
predilection of common pathological changes in this part of the human
stomach. Furthermore, there was loss of the expression of individual
isoenzymes in gastric neoplasms.
相似文献
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We describe the successful selective coil embolization of an infected superior gluteal pseudoaneurysm secondary to methicillin-resistant Staphylococcus aureus (MRSA) in a 36-year old women. The patient presented with a long history of drug abuse and perisacral abscesses due to chronic sacroilitis. The chosen strategy provides a safe and successful management of infected false gluteal artery aneurysm. 相似文献
35.
The initial event in fibrin clot formation is the thrombin-catalyzed cleavage of the A alpha chain of human fibrinogen. Most of the information required for thrombin recognition and cleavage of the A alpha chain lies in the amino terminal 51 residue CNBr fragment. By selective modification of residues in this region, we probed the features that participate in thrombin interactions. We constructed a vector which expressed a tripartite protein (tribrid) consisting of amino acids 1 to 50 of the A alpha chain followed by 60 amino acids of chicken collagen and the beta-galactosidase protein from Escherichia coli. Cell lysates run on NaDodSO4-polyacrylamide gels contained the predicted band of molecular weight (mol wt) 125,000. The tribrid reacted with a monoclonal antibody, Mab-Y18, which recognizes the amino terminus of the A alpha chain. When cell lysates were incubated with thrombin, FPA was released. By including one heterogeneous oligonucleotide in the construction, we generated plasmids that encoded three specific amino acid substitutions. Surprisingly, changing Gly14 to Val did not alter thrombin cleavage, although recognition by Mab-Y18 was lost. Substitution of lie for Arg23 did not alter either thrombin cleavage or monoclonal recognition. Substitution of Leu for Arg 16 altered thrombin cleavage; unexpectedly, recognition by Mab-Y18 was not changed. 相似文献
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W Pernice L Schuchmann J Dippell J Suschke P Vogel H Truckenbrodt F Schindera C Humburg J Brzoska 《Arthritis and rheumatism》1989,32(5):643-646
Nine severely ill patients with a confirmed diagnosis of systemic juvenile rheumatoid arthritis were treated with recombinant gamma-interferon (gamma-IFN) in addition to the therapy they were previously receiving for their disease. Improvements in clinical symptoms were noted in 7 of the patients, and median laboratory values also showed a marked improvement after gamma-IFN treatment. A relapse occurred in 1 patient. The results of this study should stimulate further research on the use of gamma-IFN in systemic juvenile rheumatoid arthritis, particularly in determining the appropriate effective dosage. 相似文献
38.
Cyril Gitiaux MD Emmanuel Roze MD PhD Kiyoka Kinugawa MD Constance Flamand‐Rouvière ST Nathalie Boddaert MD PhD Emmanuelle Apartis MD PhD Vassili Valayannopoulos MD Guy Touati MD Jacques Motte MD PhD David Devos MD PhD Karine Mention MD Dries Dobbelaere MD PhD Diana Rodriguez MD PhD Agathe Roubertie MD PhD Brigitte Chabrol MD PhD François Feillet MD PhD Marie Vidailhet MD Nadia Bahi‐Buisson MD PhD 《Movement disorders》2008,23(16):2392-2397
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大鼠脂肪间充质干细胞的成骨分化 总被引:2,自引:2,他引:2
目的:观察大鼠脂肪间充质干细胞经成骨诱导向成骨细胞分化的生物学特性,探讨其作为骨组织工程种子细胞的可行性。方法:实验于2004-07/2006-03在中南大学湘雅医院中心实验室完成主要工作。①取健康SD大鼠双侧腹股沟区脂肪垫,消化法分离出脂肪间充质干细胞,接种入含有体积分数为0.1的新生牛血清的低糖DMEM培养基进行原代培养。②取第3代的脂肪间充质干细胞,用含有体积分数为0.1的新生牛血清、0.1μmol/L地塞米松、50μmol/L抗坏血酸、10mmol/Lβ-甘油磷酸钠的高糖DMEM培养基诱导其向成骨细胞分化。③于3,5,7,10,12,14,21d分别采用倒置显微镜观察细胞形态及增殖情况、Gomori改良钙钴法碱性磷酸酶染色、茜素红S钙结节染色和Ⅰ型胶原免疫细胞化学染色检测脂肪间充质干细胞成骨分化的情况。结果:①脂肪间充质干细胞原代细胞呈成纤维细胞样长梭形外观,传代稳定,细胞形态均一。②经成骨诱导,脂肪间充质干细胞体积增大,呈多角形;成骨诱导14d,Gomori改良钙钴法碱性磷酸酶染色,细胞胞浆内可见浅棕色至棕黑色的颗粒,平均染色阳性率为80%;碱性磷酸酶活性随时间的延长而逐渐增高[3,5,7,10,12,14d依次为(2.43±0.09),(3.60±0.08),(5.01±0.09),(7.75±0.07),(9.59±0.09),(10.94±0.10)μkat/L];成骨诱导21d,钙结节形成明显,茜素红S染色,呈红色结节;成骨诱导7d,Ⅰ型胶原免疫细胞化学染色,细胞胞浆呈棕黄色,胞核经苏木精复染为蓝色。结论:大鼠脂肪间充质干细胞经成骨诱导具有成骨细胞的生物学特性,可作为骨组织工程的种子细胞。 相似文献