Pathologische Anatomie (Sektionstechnik) und Physiologie

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Zur Behandlung der Fractura humeri supracondylica mittels Gelenkautoplastik

Ohne Zusammenfassung (Mit 21 Abbildungen.) Vortrag, gehalten auf der 85. Versammlung deutscher Naturforscher und ?rzte in Wien in der Sektion für Chirurgie am 24. IX. 1913.     

Therapie der Syphilis

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Tödlichen Arsenwassertoff-Vergiftung bei Galvanisierungsarbeiten

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碱性磷酸酶-抗碱性磷酸酶铅沉淀技术及其优化

本文介绍了用以显示碱性磷酸酶活性的铅沉淀法的碱性磷酸酶-抗碱性磷酸酶(APA-AP)免疫组织化学技术,以及使用计算机图像处理进行显微光密度测量确定的该技术理想反应条件.其条件是:孵育液硝酸铅浓度为3mmol/L,底物浓度1.96～31.36mmol/L,pH值7～9;APAAP浓度1:50;一级抗体、第一和第二桥抗体的浓度分别为1:1 000、1:100和1:200。讨论了APAAP-铅沉淀技术的某些优点。     

Histochemical localization of adrenergic nerves in the guinea-pig trachea

1. Specific catecholamine fluorescence was demonstrated in guinea-pig trachea in fine, varicose, nerve fibres running parallel to the tracheal smooth muscle fibres.2. The density of nerves in tracheal smooth muscle was greater at the laryngeal end than at the bronchial end of the trachea.3. The findings confirm pharmacological evidence for an adrenergic innervation of the guinea-pig isolated tracheal chain preparation.     

A histochemical study of extraneuronal accumulation of noradrenaline in the guinea-pig trachea

1. Accumulation of noradrenaline in extraneuronal tissue of the guinea-pig trachea has been studied by the use of the fluorescence histochemical technique of Falck and Hillarp.2. After incubation in solutions of noradrenaline, fluorescence developed in cellular structures (tracheal smooth muscle, vascular smooth muscle and endothelium, fibroblasts and chondroblasts), in the intercellular matrix of the cartilage and throughout the loose connective tissue of the adventitia and submucosa.3. The effect of various experimental procedures on the development of this fluorescence has been examined i.e. incubation with noradrenaline at reduced temperature (0 degrees C), removal of fluorescence by washing with Krebs solution at 37 degrees C or 0 degrees C, incubation with phenoxybenzamine (10(-4)M) or metanephrine (10(-4)M).4. From these observations it has been concluded that noradrenaline accumulates in the trachea:(a) in cellular structures where it is firmly bound, i.e. not easily removed by washing at 0 degrees C, and where the accumulation is prevented by phenoxybenzamine, metanephrine or cold.(b) in the intercellular matrix of the cartilage where it is also firmly bound, but where the accumulation is not prevented by phenoxybenzamine, metanephrine or cold. This probably represents binding to sulphated mucopolysaccharides.(c) in the adventitia and submucosa where it is loosely bound and easily removed by washing.5. Some implications of these findings in pharmacological experiments with guinea-pig trachea are discussed.     

Die gefährliche Alkoholkonzentration

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