跨声门癌50例观察

为确立一种原发喉室的跨声门癌，选择肿瘤主体以喉室为中心经全喉切除手术的标本５０例，火棉胶包埋，连续切片观察。结果发现①跨声门癌的肿瘤原发部位在喉室，５０例中有Ｔ２３例。②肿瘤以深层浸润为主占９０％（４５／５０），易侵及声门旁间隙（８２％，４１／５０）和甲状软骨（６４％，３２／５０），临床分期与病理分期不符达４８％（２４／５０）。结论：原发喉室的跨声门癌是声门上型癌的一种特殊类型。     

HPLC测定佛手增乳膏中阿魏酸的含量

佛手增乳膏经乙醚，2％碳酸钠液萃取后，用HPLC测定阿魏酸的含量。方法简便，快速，准确。     

喉近全切除喉功能重建术

目的 为了减少喉全切除率并重建喉功能。方法 自１９９１￣１９９６年作喉近全切除喉功能重建术１９例。男８例，女１１例。年龄最大７４岁，最小４０岁，平均５７．６岁。临床分期Ⅱ期２例，Ⅲ期９例，Ⅳ期８例。手术特点是：切除舌骨，保留环状软骨及一侧杓状软骨，将五状软骨前缘与舌根切缘吻合，增强了舌根对新喉口的遮盖作用，减轻了误咽．结果 全部病例术后发音功能良好，多数病例误咽不重。５例拔除套管经喉呼吸。１４例新     

DIAGNOSIS AND TREATMENT OF MEDIASTINAL ENTEROGENOUS CYSTS IN CHILDREN

MEDIASTINAL enterogenous cysts are congen-ital anomalies rarely seen in the pediatric agegroups.Most children are first admitted tohospital due to respiratory symptoms.Neonates and littleinfants often have the symptom of respiratory distress,while a few a…     

城市建筑屋面绿化技术研究

本文全面系统地介绍了现代城市建筑中的屋面绿化技术,主要包括屋面构造、植物选型、栽培质选型、蓄排水选型、抗根防水层选型、保温层选型等内容,并介绍了几种不同的经济合理的绿化屋面构造方案,适用于新建及旧建筑中应用。通过系统地研究对屋面绿化技术,能有助于各地推广及应用屋面绿化技术。     

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目的 探讨先天性心脏病(CHD)患儿肺动脉高压(PH)形成的影响因素.方法 研究对象均为2003-06-2005-02于北京大学深圳医院收集病例,以健康者30名为对照组(A组),以肺动脉压正常和并发PH的左向右分流的CHD患儿各30例为观察组(B组、C组).以高效液相色谱法、硝酸还原法及放射免疫法测定其血清精氨酸(L-Arg)、一氧化氮(NO)、血浆内皮素(ET-1)的浓度.结果 血清L-Arg浓度对照组(A组)为(72.00±18.01)nmol/mL,肺动脉压正常的患儿(B组)为(30.74±8.97)nmol/mL,伴PH的患儿(C组)为(23.51±12.37)nmol/mL.血清NO浓度A组为(76.10±17.10)nmol/mL,B组(90.55±26.57)nmol/mL,C组(60.05±17.60)nmol/mL.血浆ET-1浓度A组(50.82±7.58)pg/mL,B组(64.90±16.28)pg/mL,C组(69.64±10.66)pg/mL.结论 血清NO浓度和血浆ET-1浓度及其之间的平衡关系共同影响PH的形成及其程度.血浆ET-1浓度的升高是肺动脉压升高的直接因素,血清NO浓度的降低是间接因素,而血清NO浓度降低是由血清L-Arg浓度的降低引起.     

Immune responses against SARS-coronavirus nucleocapsid protein induced by DNA vaccine

The nucleocapsid （N） protein of SARS-coronavirus （SARS-CoV） is the key protein for the formation of the helical nucleocapsid during virion assembly. This protein is believed to be more conserved than other proteins of the virus, such as spike and membrane glycoprotein. In this study, the N protein of SARS-CoV was expressed in Escherichia coli DHSalpha and identified with pooled sera from patients in the convalescence phase of SARS. A plasmid pCI-N, encoding the full-length N gene of SARS-CoV, was constructed. Expression of the N protein was observed in COS1 cells following transfection with pCI-N. The immune responses induced by intramuscular immunization with pCI-N were evaluated in a murine model. Serum anti-N immunoglobutins and splenocytes proliferative responses against N protein were observed in immunized BALB/c mice. The major immunoglobulin G subclass recognizing N protein was immunoglobulin G2a, and stimulated splenocytes secreted high levels of gamma interferon and IL-2 in response to N protein. More importantly, the immunized mice produced strong delayed-type hypersensitivity （DTH） and CD^8＋ CTL responses to N protein.     

关于802.1X认证技术在校园网应用中问题的探讨

介绍了802.1X认证技术的体系结构和工作原理,分析了802.1X认证技术在校园网中应用的优势与不足.     

Metabolic activation of fluoropyrrolidine dipeptidyl peptidase-IV inhibitors by rat liver microsomes.

The current study evaluated the potential for two dipeptidyl peptidase-IV (DPP-IV) inhibitor analogs (1S)-1-(trans-4-([(4-trifluoromethoxyphenyl)sulfonyl]amino)cyclohexyl)-2-[(3S)-3-fluoropyrrolidin-1-yl]-2-oxoethanaminium chloride and (1S)-1-(trans-4-([(2,4-difluorophenyl)sulfonyl]amino)cyclohexyl)-2-[(3S)-3-fluoropyrrolidin-1-yl]-2-oxoethanaminium chloride (MRL-A and MRL-B), containing a fluoropyrrolidine moiety in the structure, to undergo metabolic activation. The irreversible binding of these tritium-labeled compounds to rat liver microsomal protein was time- and NADPH-dependent and was attenuated by the addition of reduced glutathione (GSH) or N-acetylcysteine (NAC) to the incubation, indicating that chemically reactive intermediates were formed and trapped by these nucleophiles. Mass spectrometric analyses and further trapping experiments with semicarbazide indicated that the fluoropyrrolidine ring had undergone sequential oxidation and defluorination events resulting in the formation of GSH or NAC conjugates of the pyrrolidine moiety. The bioactivation of MRL-A was catalyzed primarily by rat recombinant CYP3A1 and CYP3A2. Pretreatment of rats with prototypic CYP3A1 and 3A2 inducers (pregnenolone-16alpha-carbonitrile and dexamethasone) enhanced the extent of bioactivation which, in turn, led to a higher degree of in vitro irreversible binding to microsomal proteins (5- and 9-fold increase, respectively). Herein, we describe studies that demonstrate that the fluoropyrrolidine ring is prone to metabolic activation and that GSH or NAC can trap the reactive intermediates to form adducts that provide insight into the mechanisms of bioactivation.