全文获取类型
收费全文 | 4904篇 |
免费 | 555篇 |
国内免费 | 19篇 |
专业分类
耳鼻咽喉 | 36篇 |
儿科学 | 203篇 |
妇产科学 | 140篇 |
基础医学 | 683篇 |
口腔科学 | 131篇 |
临床医学 | 589篇 |
内科学 | 1092篇 |
皮肤病学 | 51篇 |
神经病学 | 425篇 |
特种医学 | 232篇 |
外科学 | 579篇 |
综合类 | 124篇 |
一般理论 | 5篇 |
预防医学 | 534篇 |
眼科学 | 76篇 |
药学 | 373篇 |
中国医学 | 7篇 |
肿瘤学 | 198篇 |
出版年
2021年 | 60篇 |
2019年 | 64篇 |
2018年 | 96篇 |
2017年 | 75篇 |
2016年 | 61篇 |
2015年 | 55篇 |
2014年 | 75篇 |
2013年 | 147篇 |
2012年 | 227篇 |
2011年 | 192篇 |
2010年 | 137篇 |
2009年 | 109篇 |
2008年 | 142篇 |
2007年 | 189篇 |
2006年 | 150篇 |
2005年 | 178篇 |
2004年 | 165篇 |
2003年 | 154篇 |
2002年 | 164篇 |
2001年 | 150篇 |
2000年 | 151篇 |
1999年 | 130篇 |
1998年 | 76篇 |
1997年 | 57篇 |
1996年 | 52篇 |
1995年 | 57篇 |
1993年 | 61篇 |
1992年 | 90篇 |
1991年 | 89篇 |
1990年 | 92篇 |
1989年 | 124篇 |
1988年 | 117篇 |
1987年 | 106篇 |
1986年 | 119篇 |
1985年 | 117篇 |
1984年 | 71篇 |
1983年 | 83篇 |
1982年 | 63篇 |
1981年 | 68篇 |
1979年 | 97篇 |
1978年 | 74篇 |
1977年 | 53篇 |
1976年 | 73篇 |
1975年 | 57篇 |
1974年 | 80篇 |
1973年 | 63篇 |
1972年 | 68篇 |
1971年 | 61篇 |
1970年 | 61篇 |
1969年 | 57篇 |
排序方式: 共有5478条查询结果,搜索用时 15 毫秒
71.
Distinctions between endemic and sporadic forms of Epstein-Barr virus-positive Burkitt's lymphoma 总被引:12,自引:0,他引:12
M Rowe C M Rooney A B Rickinson G M Lenoir H Rupani D J Moss H Stein M A Epstein 《International journal of cancer. Journal international du cancer》1985,35(4):435-441
Tumour cell lines were established in vitro from 16 cases of Epstein-Barr (EB) virus genome-positive Burkitt's lymphoma (BL), 7 of "endemic" origin (i.e. from holoendemic malarial areas of Africa and of New Guinea) and 9 of "sporadic" origin (i.e. from outside such high-incidence areas). All the BL cell lines thus established were monoclonal by immunoglobulin isotype expression and displayed a characteristic chromosomal translocation, t(8:14) or t(8:22), confirming their malignant origin. Clear differences observed between the individual BL cell lines appeared to be related to their endemic or sporadic status. All 7 endemic cell lines began growth as a carpet of single cells, often with small, loose clumps appearing in later passage. Whilst 3 lines of sporadic origin displayed a similar pattern to the above, the majority of sporadic lines grew as large, tight clumps of cells from the first passage onwards. These differences in growth pattern were reflected by differences in cell surface phenotype, as defined in indirect immunofluorescence tests using a panel of monoclonal antibodies (MAbs) specific for B-lineage-associated antigens. BL cell lines could be classified into 3 separate groups on the basis of their reactivity with 6 particular antibodies (MHM6, AC2, Ki-1, Ki-24, J5 and 38.13). All 7 endemic BL cell lines and 2 of the 3 sporadic BL cell lines which began growth as single cells showed a group-I cell-surface phenotype (MHM6, AC2, Ki-1, Ki-24 negative; J5, 38.13 positive) in early passage. In contrast, all 6 sporadic BL cell lines which began growth in large clumps displayed a distinct group-II phenotype (MHM6, AC2, Ki-1 positive/negative; Ki-24, J5, 38.13 positive); in later passage most of these sporadic lines progressed to a group-III phenotype (MHM6, AC2, Ki-1, Ki-24 positive; J5, 38.13 negative) without loss of those immunoglobulin and chromosomal markers identifying the cells' malignant origin. These clear differences between endemic BL cell lines on the one hand and the majority of sporadic BL cell lines on the other suggest that endemic BL arises from a more restricted range of progenitor B cells than does the sporadic form of the disease. 相似文献
72.
W M Rowe 《Radiology management》1986,8(4):34-36
Regardless of the amount of information available today relative to radiology management information systems, the manager with minimum experience needs to "focus" attention on specific aspects of computerization during the selection and implementation processes. This article defines those areas of interest that can contribute to the overall success and acceptance of a system. 相似文献
73.
74.
R. Lange P. Rowe L. Seebauer J. Mathews J. R. Siewert W. Silen 《Langenbeck's archives of surgery / Deutsche Gesellschaft fur Chirurgie》1984,364(1):539-539
Zusammenfassung Der Mechanismus der Cytoprotektion von Prostaglandin (PG) auf die Magenmucosa ist unbekannt. Anaesthesierte Kaninchen erhielten in der Gruppe (GR) I (n=8) im. Aspirin (ASA) (100 mg/kg als Bolus, 66 mg/kg/h kontinuierlich) in der GR 11 (n=10) NaCI und in der GR III (n=7) zusätzlich zum ASA, PGE I (0,1 g/kg/min) als Infusion über 120 min. Der Mucosa-Blutfluß (MBF) wurde mit radioaktiven Mikrosphären gemessen. In der GR I fiel der MBF nach 15 min um 72,3+3,8% (+ SEM) und nach 120 min um 73,1+3,8% (p 0,05 gegen % Änderung in GR II und III). Nach 120 min zeigte sich in der GR I 19,8 + 7,6% der Fundusmucosa hämorrhagisch, in der GR 11 6,1 +5,2% und in der GR 1112,0+ 1,4% (p 0,05 gegen GR II und III). Wir schließen, daß der cytoprotektive Effekt des PGE I auf einer Aufhebung der ASA bedingten Mucosaischämie beruht. 相似文献
75.
76.
Carsten Schwarz Sivagurunathan Sutharsan Ralph Epaud Ross C. Klingsberg Rainald Fischer Steven M. Rowe Paul K. Audhya Neil Ahluwalia Xiaojun You Thomas J. Ferro Margaret E. Duncan Bote G. Bruinsma 《Journal of cystic fibrosis》2021,20(2):228-233
BackgroundIncreased rates of respiratory adverse events have been observed in people ≥12 years of age with cystic fibrosis homozygous for the Phe508del-CFTR mutation treated with lumacaftor/ivacaftor, particularly in those with percent predicted forced expiratory volume in 1 s (ppFEV1) of <40%. We evaluated the safety, tolerability, and efficacy of tezacaftor/ivacaftor in people with cystic fibrosis homozygous for Phe508del-CFTR who discontinued lumacaftor/ivacaftor due to treatment-related respiratory signs or symptoms.MethodsParticipants ≥12 years of age with cystic fibrosis homozygous for Phe508del-CFTR with ppFEV1 of ≥25% and ≤90% were randomized 1:1 and treated with tezacaftor/ivacaftor or placebo for 56 days.ResultsOf 97 participants, 94 (96.9%) completed the study. The primary endpoint was incidence of predefined respiratory adverse events of special interest (chest discomfort, dyspnea, respiration abnormal, asthma, bronchial hyperreactivity, bronchospasm, and wheezing): tezacaftor/ivacaftor, 14.0%; placebo, 21.3%. The adverse events were mild or moderate in severity. None were serious or led to treatment interruption or discontinuation. Overall, the discontinuation rate was similar between groups. The mean (SD) ppFEV1 at baseline was 44.6% (16.1%) with tezacaftor/ivacaftor and 48.0% (18.1%) with placebo. The posterior mean difference in absolute change in ppFEV1 from baseline to the average value of days 28 and 56 was 2.7 percentage points with tezacaftor/ivacaftor vs placebo.ConclusionsTezacaftor/ivacaftor was generally safe, well tolerated, and efficacious in people ≥12 years of age with cystic fibrosis homozygous for Phe508del-CFTR with ppFEV1 of ≥25% and ≤90% who previously discontinued lumacaftor/ivacaftor due to treatment-related respiratory signs or symptoms. 相似文献
77.
Shamsah Kazani David J. Rowlands Ivan Bottoli Julie Milojevic Jose Alcantara Ieuan Jones Kenneth Kulmatycki Surendra Machineni Lidia Mostovy Ian Nicholls Jerry A. Nick Steven M. Rowe Nicholas J. Simmonds Raju Vegesna Jeroen Verheijen Henry Danahay Martin Gosling Phaninatha Sarma Ayalavajjala Robert Strieter 《Journal of cystic fibrosis》2021,20(2):250-256
BackgroundThis is the first-in-human study of icenticaftor, an oral potentiator of the cystic fibrosis (CF) transmembrane conductance regulator (CFTR) channel. Restoration of CFTR activity has shown significant clinical benefits, but more studies are needed to address all CFTR mutations.MethodsSafety, pharmacodynamics/pharmacokinetics of icenticaftor were evaluated in a randomized, double-blind, placebo-controlled study in healthy volunteers. Efficacy was assessed in adult CF patients with ≥1 pre-specified CFTR Class III or IV mutation (150 and 450 mg bid), or homozygous for F508del mutation (450 mg bid). Primary efficacy endpoint was change from baseline in lung clearance index (LCI2.5). Secondary endpoints included %predicted FEV1 and sweat chloride level.ResultsClass IV mutations were present in 22 patients, Class III in 2 (both S549N), and 25 were homozygous for F508del. Icenticaftor was well-tolerated in healthy and CF subjects with no unexpected events or discontinuations in the CF groups. The most frequent study-drug related adverse events in CF patients were nausea (12.2%), headache (10.2%), and fatigue (6.1%). Icenticaftor 450 mg bid for 14 days showed significant improvements in all endpoints versus placebo in patients with Class III and IV mutations; mean %predicted FEV1 increased by 6.46%, LCI2.5 decreased by 1.13 points and sweat chloride decreased by 8.36 mmol/L. No significant efficacy was observed in patients homozygous for a single F508del.ConclusionsIcenticaftor was safe and well-tolerated in healthy volunteers and CF patients, and demonstrated clinically meaningful changes in lung function and sweat chloride level in CF patients with Class III and IV CFTR mutations.ClinicalTrials.gov: NCT02190604 相似文献
78.
Dave P. Nichols Scott H. Donaldson Carla A. Frederick Steven D. Freedman Daniel Gelfond Lucas R. Hoffman Andrea Kelly Michael R. Narkewicz Jessica E. Pittman Felix Ratjen Scott D. Sagel Margaret Rosenfeld Sarah Jane Schwarzenberg Pradeep K. Singh George M. Solomon Michael S. Stalvey Shannon Kirby Jill M. VanDalfsen Steven M. Rowe 《Journal of cystic fibrosis》2021,20(2):205-212
Highly effective CFTR modulator drug therapy is increasingly available to those with cystic fibrosis. Multiple observational research studies are now being conducted to better understand the impacts of this important therapeutic milestone on long-term outcomes, patient care needs, and future research priorities. PROMISE is a large, multi-disciplinary academic study focused on the broad impacts of starting elexacaftor/tezacaftor/ivacaftor in the US population age 6 years and older. The many areas of investigation and rationale for each are discussed by organ systems, along with recognition of remaining important questions that will not be addressed by this study alone. Knowledge gained through this and multiple complementary studies around the world will help to understand important health outcomes, clinical care priorities, and research needs for a large majority of people treated with these or similarly effective medications targeting the primary cellular impairment in cystic fibrosis. 相似文献
79.
80.
- The actions of several neuroleptic and tricyclic compounds were examined on the large conductance Ca2+-activated K+ (BKCa) channel present in neurones isolated from the rat motor cortex.
- Classical neuroleptic compounds including chlorpromazine and haloperidol applied to the intracellular surface of inside-out patches produced a concentration-dependent reduction in BKCa channel activity. Similar effects were observed when these compounds were applied to the extracellular surface of outside-out patches.
- In contrast, the atypical neuroleptic compounds clozapine and sulpiride did not affect BKCa channel activity (100 nM–1 mM) in either inside-out or outside-out patches, while 10 μM pimozide produced 73% of the inhibition produced by 10 μM chlorpromazine.
- BKCa channel activity was also unaffected by application of structurally related tricyclic compounds including the anti-cholinesterase tacrine and the anti-epileptic carbamazepine. The tricyclic antidepressant drug amitriptyline was found to inhibit BKCa channel activity but was much less effective than the classical neuroleptic compounds.
- It is concluded that compounds belonging to the classical neuroleptic group of drugs inhibit BKCa channel activity in the rat motor cortex in a structurally-specific manner. This observation may be of clinical significance as it may contribute to some of the side effects associated with classical neuroleptic drug therapy.