杆状病毒介导LacZ基因转染体外培养的人虹膜色素上皮细胞

目的：研究重组杆状病毒(baculovinus)载体介导β—半乳糖苷酶基因(LacZ)对体外培养虹膜色素上皮(IPE)细胞的转染和表达情况。方法：将质粒pCMV—β中的CMV—LacZ表达盒插入杆状病毒表达栽体pFast BacI的Eco RI／Hind Ⅲ位点，构建重组质粒pBac—β，并利用Bac—to—Bac表达系统在Sf9细胞中产生出重组杆状病毒BV—β。将MOI为200的BV—β感染经酶消化加显微法分离培养的IPE细胞，24h后进行X-gal染色。在显微镜下观察IPE中LacZ表达阳性的情况，记录阳性细胞所占的百分比。结果：限制性酶切分析及测序结果表明，我们已成功构建表达质粒pBac—β及重组杆状病毒BV—β。X-gal染色证实杆状病毒介导LacZ在细胞中的表达呈蓝色，弥漫于整个胞浆。感染后24h时表达阳性率达85％。结论：重组杆状病毒可有效介导报告基因在体外培养的IPE细胞中表达，为利用杆状病毒构建可供移植的基因工程化虹膜色素上皮细胞提供了实验依据。     

造血干细胞移植病人出血性膀胱炎的危险因素分析

[目的]了解造血干细胞移植病人出血性膀胱炎发生的危险因素。[方法]对29例造血干细胞移植病人出血性膀胱炎的危险因素进行统计学分析。[结果]出血性膀胱炎的发生率34％(10/29例),经Logistic回归分析发现出血性膀胱炎的危险因素是预处理方案(OR值0.058 0),马利兰/环磷酰胺预处理方案出血性膀胱炎发生率高。[结论]预处理方案对出血性膀胱炎的发生有重要影响。     

宫腔声学造影在诊断及处理宫内节育器嵌顿中的价值

目的 评价宫腔声学造影（SHG）在诊断及处理宫内节育器（IUD）嵌顿中的价值。方法 应用SHG对18例疑IUD嵌顿的带器患者进行检查，并根据SHG检查结果予以相应处理。结果 SHG显示IUD浅嵌顿10例、深嵌顿4例、IUD下移1例及双器1例。除1例放弃取器外，17例病人经适当手术方法取出IUD。结论 宫腔声学造影是诊断IUD嵌顿的一种简便、有效的新方法，其结果有助于临床处理中取器方法的选择。     

云纹技术在断裂分析中的应用

改进了传统的云纹数据处理方法,提出用曲面拟合方法处理云纹数据,提高了计算机处理云纹数据的效率与质量。又提出用云纹法测量J积分的技术,可望解决实际工程构件的J积分测量问题。     

Surgical treatment of hemolytic anemia

The therapeutic effect of splenectomy in 70 cases of hemolytic anemia are reported. The diagnosis and therapeutic effects were analysed. It was evident that the effects were related to different types of hemolytic anemia. The HS type had the best result. Gallstones were detected in 6 cases of this series. Splenectomy and cholecystectomy were performed together in 5 cases. The median incision of upper abdomen was selected. The authors believe that the causes of infection after splenectomy are complicated and they cannot be explained by Igs alone.     

TD清洗消毒剂对口腔科器械消毒效果探讨

通过实验室和口腔科临床观察表明:TD清洗消毒剂(氯化磷酸三钠)对金黄色葡萄球菌、绿脓杆菌和枯草杆菌黑色变种的平均杀菌率为100.0％,对口腔科器械消毒无菌率为100％,对被HBsAg阳性血清污染的器械浸泡30分钟转阴率为98.1％。其消毒效果优于新洁尔灭,与戊二醛相同。在口腔科使用两年,未见金属器械有锈斑生成。     

ROBUST GUARANTEED COST CONTROL OF UNCERTAIN NON-LINEAR ROBOTIC SYSTEM USING MIXED MINIMUM TIME AND QUADRATIC PERFORMANCE INDEX

To provide better transient performance in controlling robots, a mixed minimum time and quadratic performance index is used. For known and uncertain revolute robot manipulators a conventional optimal control and a robust guaranteed cost control are proposed respectively. Asymptotic stability is also discussed based on the Lyapunov direct method. The proposed robust control is continuous and requires no knowledge of the uncertain system except for bounding functions.     

NGF prevents the changes induced by monocular deprivation during the critical period in rats

Photic evoked responses were recorded from the striate cortex of Long-Evans hooded intact, monocular visual deprivation (MD) and MD treated with NGF rats. The averaged visual evoked responses (AVER) were obtained from both hemispheres and provided comparison after binocular photic stimuli between the contralateral and the ipsilateral striate cortex with relation to the MD eye. One month of monocular visual deprivation at the critical period of development resulted in marked reduction of the amplitudes of AVER components as compared to the control recordings (P < 0.001). These changes of the AVER could be prevented by NGF infusion to lateral ventricle at the dosage of 2.0–2.4 μg/day for four weeks during the monocular deprivation. In conclusion, the change of AVER amplitudes induced by monocular visual deprivation during the critical period of development can be prevented by NGF infusion to lateral ventricle.     

吡那地尔对高血压心脏结构和功能重构的影响

在等降压剂量下吡那地尔和赖诺普利可使４月龄自发性高血压大鼠的血压下降６．０￣８．０ｋＰａ，并接近同种属正常血压大刀瓣血压水平。