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141.
0 引言 应用免疫荧光组织化学方法结合激光扫描共聚焦显微镜 ( LSCM) ,观察了人乳头瘤病毒 HPV1 6型 ( Human pa-pillomavirus type1 6) E6,E7基因区表达的 E6,E7蛋白产物在宫颈癌细胞系 Si Ha中的表达情况 .1 材料和方法1 .1 细胞培养 含 HPV基因组织的宫颈癌细胞系 Si Ha由西安医科大学分子生物研究中心李旭教授提供 ,细胞于含 1 0 0m L· L- 1 胎牛血清 (美国 Gibco)的 RPMI-1 640培养基 (美国Gibco)常规培养 .将细胞悬液滴于盖玻片上培养 ,制成单层细胞爬片 .1 .2 免疫荧光染色 正常羊血清封闭 30 min,分别加入鼠抗H… 相似文献
142.
Integrated approach to oral health in aged care facilities using oral health practitioners and teledentistry in rural Queensland 下载免费PDF全文
143.
Characterization and Expression of the Human T Cell Receptor-T3 Complex by Monoclonal Antibody F101.01 总被引:5,自引:0,他引:5
C. GEISLER T. PLESNER G. PALLESEN K. SKJØDT N. ØDUM J. K. LARSEN 《Scandinavian journal of immunology》1988,27(6):685-696
A murine monoclonal antibody (MoAb) F101.01 reacting with the T cell receptor (TCR)-T3 complex is presented. Immunohistological studies showed that F101.01 specifically stains T-zone lymphocytes in lymph nodes, tonsils, and splenic tissue. Two-colour immunofluorescence and flow cytometry demonstrated co-expression of the antigen defined by F101.01 and the pan-T cell antigens defined by CD2, CD3, CD5, and CD7 antibodies. Cells stained with CD4 and CD8 antibodies were both included in the F101.01-positive population, whereas CD16-positive natural killer cells (NK), B cells (CD19 and CD20), and myeloid cells (CD13 and CD33) were excluded. The target antigen of F101.01 co-modulated with the CD3-defined antigen (T3) and the TCR recognized by the MoAb WT-31. CD3 antibody and WT-31 both blocked binding of F101.01. F101.01 precipitated the TCR-T3 complex from lysates of 125I-labelled peripheral blood mononuclear cells (PBMC) and HPB-ALL, when the lysate was prepared with a detergent (digitonin) that conserves the TCR-T3 complex. FACS analysis of T cells from a patient with a T cell immunodeficiency demonstrated that delta-TCS-1-CD3+CD4+ and delta-TCS-1-CD3+CD8+ cells were brightly F101.01+, whereas a large subpopulation of delta-TCS-1+CD3+CD4-CD8- cells were weakly F101.01+. We conclude that F101.01 recognizes a conformational epitope of the TCR-T3 complex and that it reacts with the alpha beta TCR-T3 and the gamma delta TCR-T3 complexes with different intensities. 相似文献
144.
The analysis of fulfilling nutrition needs on the stages of family evolution process has been recently carried out. It was based on GUS examining of households' budgets. The result was that in some households people consume not enough carbohydrate, fiber, calcium, copper and B1 vitamin. The families with children were especially endangered by low level of such ingredients. Their diets were rich in fat, cholesterol, sodium and phosphorus. 相似文献
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Syphilis is a sexually transmitted infectious disease caused by Treponema pallidum. Cases of syphilis have increased in frequency and are challenging when affecting the elderly. The main causes of increased prevalence of syphilis are sexual promiscuity, lack of knowledge about the disease and decreasing use of barrier protection. Clinically, the oral manifestation of syphilis may resemble other entities, which hampers the correct diagnosis. We report a case of a 79‐year‐old male with weight loss and feeding difficulties. In the oral cavity there were ulcerative lesions in the hard palate and bilaterally in the buccal mucosa. The incisional biopsy revealed only a non‐specific ulceration of the oral mucosa. After 20 days, the patient was re‐evaluated and presented maculopapular lesions in the palmar and plantar areas. Positive serological venereal disease reference laboratory (VDRL) tests confirmed the diagnosis of secondary syphilis. The patient was treated with Benzathine penicillin G. After two weeks of treatment the oral lesion disappeared and the patient returned to normal feeding and gained weight. This case report reinforces the need to alert physicians and dentists to include sexually transmitted infections such as syphilis in the differential diagnosis of oral ulcerative lesions in elderly sexually active patients. 相似文献
147.
NA Afzal MU Shenoy S Haque DT Wilcox N Shah 《Acta paediatrica (Oslo, Norway : 1992)》2010,99(7):1042-1046
Although genitourinary complications of Crohn’s are well recognized, available information regarding their clinical course and management is sparse especially in the paediatric population. We report a myriad of urological complications in five paediatric cases, our experience from a tertiary paediatric urological and gastroenterological centre. All children with urological complications had severe Crohn’s disease which necessitated the use of immuno‐suppressants including Infliximab. Three of four children healed and closed their fistulas after treatment, although failed to avoid future surgery, albeit for other reasons. Conclusion: We suggest Infliximab should be considered as a treatment option but in the absence of a common consensus, treatment be tailored to individual cases. 相似文献
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BACKGROUND Microglial inhibition may reduce secondary tissue injury and improve functional outcome following acute brain injury.Utilizing clinically relevant murine models of traumatic brain injury and intracerebral hemorrhage,neuroinflammatory responses and functional outcome 相似文献