Background: General anesthetics inhibit evoked release of classic neurotransmitters. However, their actions on neuropeptide release in the central nervous system have not been well characterized.
Methods: The effects of representative intravenous and volatile anesthetics were studied on the release of sulfated cholecystokinin 8 (CCK8s), a representative excitatory neuropeptide, from isolated rat cerebrocortical nerve terminals (synaptosomes). Basal, elevated KCl depolarization-evoked and veratridine-evoked release of CCK8s from synaptosomes purified from rat cerebral cortex was evaluated at 35[degrees]C in the absence or presence of extracellular Ca2+. CCK8s released into the incubation medium was determined by enzyme-linked immunoassay after filtration.
Results: Elevation of extracellular KCl concentration (to 15-30 mm) or veratridine (10-20 [mu]m) stimulated Ca2+-dependent CCK8s release. Basal, elevated KCl- or veratridine-evoked CCK8s release was not affected significantly by propofol (12.5-50 [mu]m), pentobarbital (50 and 100 [mu]m), thiopental (20 [mu]m), etomidate (20 [mu]m), ketamine (20 [mu]m), isoflurane (0.6-0.8 mm), or halothane (0.6-0.8 mm). 相似文献
本文报道1例用丝裂霉素C进行的小梁切除术后1只眼发展为虹膜色素缺陷瘤的患者。对1例12岁的男孩进行完全的眼科检查以及遗传学测试。无全身1型神经纤维瘤病表现的患者在小梁切除术后可能发展为虹膜色素缺陷瘤。小梁切除术后虹膜色素缺陷瘤@Weizer J.S.
@Freedman S.F.$Duke University Eye Center, Box 3802 DUMC, Durham, NC 27710, United States ,Dr.
@王海燕 相似文献