全文获取类型
收费全文 | 1170篇 |
免费 | 78篇 |
国内免费 | 11篇 |
专业分类
耳鼻咽喉 | 8篇 |
儿科学 | 11篇 |
妇产科学 | 8篇 |
基础医学 | 182篇 |
口腔科学 | 9篇 |
临床医学 | 124篇 |
内科学 | 247篇 |
皮肤病学 | 20篇 |
神经病学 | 123篇 |
特种医学 | 124篇 |
外科学 | 148篇 |
综合类 | 10篇 |
预防医学 | 43篇 |
眼科学 | 4篇 |
药学 | 131篇 |
中国医学 | 1篇 |
肿瘤学 | 66篇 |
出版年
2023年 | 16篇 |
2022年 | 54篇 |
2021年 | 77篇 |
2020年 | 32篇 |
2019年 | 40篇 |
2018年 | 55篇 |
2017年 | 25篇 |
2016年 | 35篇 |
2015年 | 43篇 |
2014年 | 54篇 |
2013年 | 73篇 |
2012年 | 78篇 |
2011年 | 96篇 |
2010年 | 51篇 |
2009年 | 40篇 |
2008年 | 59篇 |
2007年 | 64篇 |
2006年 | 55篇 |
2005年 | 50篇 |
2004年 | 41篇 |
2003年 | 43篇 |
2002年 | 29篇 |
2001年 | 8篇 |
2000年 | 10篇 |
1999年 | 5篇 |
1998年 | 8篇 |
1997年 | 3篇 |
1991年 | 4篇 |
1990年 | 4篇 |
1989年 | 4篇 |
1988年 | 4篇 |
1987年 | 6篇 |
1986年 | 6篇 |
1985年 | 7篇 |
1984年 | 9篇 |
1983年 | 3篇 |
1980年 | 4篇 |
1979年 | 7篇 |
1978年 | 4篇 |
1977年 | 6篇 |
1976年 | 6篇 |
1975年 | 3篇 |
1972年 | 2篇 |
1971年 | 3篇 |
1970年 | 2篇 |
1940年 | 2篇 |
1938年 | 2篇 |
1937年 | 2篇 |
1932年 | 2篇 |
1928年 | 3篇 |
排序方式: 共有1259条查询结果,搜索用时 15 毫秒
31.
Over the last two decades quality of life (QoL) and the social challenges of allogeneic hematopoietic stem cell transplant (allo-HSCT) survivors have been emerging as subjects of extensive research and are now considered as very important aspects in the pretransplant evaluation and management of allo-HSCT recipients. Recognition of QoL challenges in allo-HSCT survivors allows timely interventions leading to improvement of post-transplant outcomes. It needs to be recognized that long-lasting life changes associated with survivorship after allo-HSCT also significantly affect QoL of partners of allo-HSCT survivors. Currently, resources should be focused on how research findings can be used by patients, their partners, and physicians to optimize QoL and psychosocial adjustment. 相似文献
32.
The role of sirtuin 2 activation by nicotinamide phosphoribosyltransferase in the aberrant proliferation and survival of myeloid leukemia cells 总被引:1,自引:0,他引:1
Dan L Klimenkova O Klimiankou M Klusman JH van den Heuvel-Eibrink MM Reinhardt D Welte K Skokowa J 《Haematologica》2012,97(4):551-559
Background
Inhibitors of nicotinamide phosphoribosyltransferase have recently been validated as therapeutic targets in leukemia, but the mechanism of leukemogenic transformation downstream of this enzyme is unclear.Design and Methods
Here, we evaluated whether nicotinamide phosphoribosyltransferase’s effects on aberrant proliferation and survival of myeloid leukemic cells are dependent on sirtuin and delineated the downstream signaling pathways operating during this process.Results
We identified significant upregulation of sirtuin 2 and nicotinamide phosphoribosyltransferase levels in primary acute myeloid leukemia blasts compared to in hematopoietic progenitor cells from healthy individuals. Importantly, specific inhibition of nicotinamide phosphoribosyltransferase or sirtuin 2 significantly reduced proliferation and induced apoptosis in human acute myeloid leukemia cell lines and primary blasts. Intriguingly, we found that protein kinase B/AKT could be deacetylated by nicotinamide phosphoribosyltransferase and sirtuin 2. The anti-leukemic effects of the inhibition of nicotinamide phosphoribosyltransferase or sirtuin 2 were accompanied by acetylation of protein kinase B/AKT with subsequent inhibition by dephosphorylation. This leads to activation of glycogen synthase kinase-3 β via diminished phosphorylation and, ultimately, inactivation of β-catenin by phosphorylation.Conclusions
Our results provide strong evidence that nicotinamide phosphoribosyltransferase and sirtuin 2 participate in the aberrant proliferation and survival of leukemic cells, and suggest that the protein kinase B/AKT/ glycogen synthase kinase-3 β/β-catenin pathway is a target for inhibition of nicotinamide phosphoribosyltransferase or sirtuin 2 and, thereby, leukemia cell proliferation. 相似文献33.
Adib A. Abla Shady Jahshan Peter Kan Maxim Mokin Travis M. Dumont Jorge L. Eller Kenneth V. Snyder L. Nelson Hopkins Adnan H. Siddiqui Elad I. Levy 《Acta neurochirurgica》2013,155(4):559-568
Background
Middle cerebral artery (MCA) aneurysms are among the more challenging aneurysms for endovascular treatment. We report a contemporary 5-year experience with endovascular therapy for MCA aneurysms at a high-volume neurovascular center.Methods
Review of prospectively maintained intracranial aneurysm database.Results
Between 2005 and 2009, 148 patients underwent treatment of 149 MCA aneurysms at our hospital, of which 33 patients with 34 aneurysms underwent endovascular therapy. Among these 33 patients, 14 presented with subarachnoid hemorrhage. Eleven patients were treated with stent-assisted coiling, 1 with balloon-assisted coiling, and the remainder with coiling alone. Three patients required repeat endovascular treatment. There were 7 periprocedural complications, including intraprocedural aneurysm rupture resulting in death in 2 patients. Two patients died at later dates from remote aneurysm rehemorrhage. Average follow-up of remaining patients was 17.1 months radiographically, and 20.3 months clinically. Average modified Rankin scale (mRS) score at last follow up was 2.09, with 17 patients with mRS 0/1 and 5 patients with mRS 2. Fifteen patients showed evidence of radiographic residual at last follow up: 13 were simple neck residuals. Unruptured status and saccular aneurysms were associated with mRS 0/1 outcome (each p?<?0.05).Conclusions
At our hospital, MCA aneurysms are being treated with endovascular techniques, but in a minority of patients. Despite the rate of residual neck remnants, few retreatments were necessary and few rehemorrhages occurred. The periprocedural complication rate was not insignificant; therefore, in more recent years and at present, most MCA aneurysms are considered for clipping first at our center. 相似文献34.
Maxim Rybalov Anthonius J. Breeuwsma Anna M. Leliveld Jan Pruim Rudi A. Dierckx Igle J. de Jong 《World journal of urology》2013,31(2):319-323
Purpose
To evaluate the effect of total PSA (tPSA) and PSA kinetics on the detection rates of 11C-Choline PET in patients with biochemical recurrence (BCR) after radical prostatectomy (RP) or external beam radiotherapy (EBRT).Methods
We included 185 patients with BCR after RP (PSA >0.2 ng/ml) or after EBRT (ASTRO definition). After injection of 400 MBq 11C-Choline i.v., a scan was made using the ECAT HR + PET camera with CT fusion images or Siemens mCT PET/CT. Biopsy-proven histology, confirmative imaging (CT or bone scan) and/or clinical follow-up (PSA) were used as composite reference. Statistical analysis was performed using PASW Statistics 18.Results
11C-Choline PET was positive in 124/185 cases (65 %) (in 22/61 (36 %) after RP, 102/124 (82 %) after EBRT). In 79 patients a local recurrence was identified, and 45 patients showed locoregional metastases on PET/CT. In 20 cases a proven false-negative PET scan was observed. Positive PET scans were confirmed by histology in 87/124 (70 %) cases, by confirmatory imaging in 34/124 (28 %) and by clinical follow-up after salvage treatment in 3 (2 %) cases. The ROC analysis to detect a recurrence showed significant difference in area under the curve (AUC) of tPSA 0.721(p < 0.001) and PSA velocity 0.730 (p < 0.001). PSA doubling time showed no significant difference with an AUC of 0.542 (p = 0.354). Detection rates are <50 % in tPSA <2 ng/ml and/or PSA velocity <1 ng/ml/year.Conclusions
Total serum PSA and PSA velocity have significant effect on the detection rates of 11C-Choline PET/CT in men with a BCR after RP or EBRT. 相似文献35.
In-Albon Tina Shafiei Maxim Christiansen Hanna Könen Tanja Gutzweiler Raphael Schmitz Julian 《Child psychiatry and human development》2022,53(6):1207-1220
Child Psychiatry & Human Development - A computer-based emotional competence inventory for preschoolers and school-aged children (MeKKi) was developed to assess five components of emotional... 相似文献
36.
37.
Ilya I. Tumkin Evgeniia M. Khairullina Maxim S. Panov Kyohei Yoshidomi Mizue Mizoshiri 《Materials》2021,14(10)
In this work, the method of selective laser reductive sintering was used to fabricate the sensor-active copper and nickel microstructures on the surface of glass-ceramics suitable for non-enzymatic detection of glucose. The calculated sensitivities for these microsensors are 1110 and 2080 μA mM−1·cm−2 for copper and nickel, respectively. Linear regime of enzymeless glucose sensing is provided between 0.003 and 3 mM for copper and between 0.01 and 3 mM for nickel. Limits of glucose detection for these manufactured micropatterns are equal to 0.91 and 2.1 µM for copper and nickel, respectively. In addition, the fabricated materials demonstrate rather good selectivity, long-term stability and reproducibility. 相似文献
38.
Maxim Cherneha Johannes Korth Meike Kaulfuß Mirko Trilling Marek Widera Hana Rohn Sebastian Dolff Nina Babel Andr Hoerning Andreas Kribben Oliver Witzke 《Viruses》2021,13(3)
Reactivations of BK polyoma virus (BKPyV) and human cytomegalovirus (HCMV) frequently cause life- and graft-threatening complications after renal transplantation. Both viruses are dependent on the mTOR pathway for replication. In this study we investigated the association of viral replication with mTOR activity in peripheral lymphocytes of renal transplant recipients. A flow-cytometry based assay for the measurement of Thr389 p70S6k phosphorylation, a surrogate marker of the mTOR pathway was established. Forty-eight adult renal transplant recipients were recruited to measure p70S6k activity in their peripheral blood mononuclear cells. This data set in conjunction with information concerning previous replication of BKPyV and HCMV was examined for correlations. Episodes of BKPyV replication were significantly associated with increased p70S6k phosphorylation in CD4+ T lymphocytes (p = 0.0002) and CD19+ B lymphocytes (p = 0.0073). HCMV infection of patients with a high-risk HCMV constellation of donor and recipient (D+/R−) was associated with increased p70S6k phosphorylation in CD19+ B lymphocytes (p = 0.0325). These associations were found to be independent of the trough levels of the immunosuppressive drugs. Conclusion: P70S6k phosphorylation in peripheral lymphocytes is associated with BKPyV reactivations and to a lesser extent with HCMV infections in renal transplant recipients. 相似文献
39.
40.