全文获取类型
收费全文 | 6228篇 |
免费 | 302篇 |
国内免费 | 45篇 |
专业分类
耳鼻咽喉 | 93篇 |
儿科学 | 152篇 |
妇产科学 | 29篇 |
基础医学 | 914篇 |
口腔科学 | 122篇 |
临床医学 | 341篇 |
内科学 | 1689篇 |
皮肤病学 | 194篇 |
神经病学 | 415篇 |
特种医学 | 157篇 |
外科学 | 996篇 |
综合类 | 11篇 |
一般理论 | 1篇 |
预防医学 | 146篇 |
眼科学 | 131篇 |
药学 | 354篇 |
中国医学 | 5篇 |
肿瘤学 | 825篇 |
出版年
2023年 | 43篇 |
2022年 | 63篇 |
2021年 | 138篇 |
2020年 | 74篇 |
2019年 | 89篇 |
2018年 | 130篇 |
2017年 | 101篇 |
2016年 | 123篇 |
2015年 | 144篇 |
2014年 | 206篇 |
2013年 | 207篇 |
2012年 | 371篇 |
2011年 | 348篇 |
2010年 | 257篇 |
2009年 | 206篇 |
2008年 | 430篇 |
2007年 | 416篇 |
2006年 | 432篇 |
2005年 | 473篇 |
2004年 | 470篇 |
2003年 | 455篇 |
2002年 | 454篇 |
2001年 | 70篇 |
2000年 | 57篇 |
1999年 | 64篇 |
1998年 | 87篇 |
1997年 | 87篇 |
1996年 | 80篇 |
1995年 | 70篇 |
1994年 | 41篇 |
1993年 | 55篇 |
1992年 | 24篇 |
1991年 | 37篇 |
1990年 | 33篇 |
1989年 | 35篇 |
1988年 | 14篇 |
1987年 | 11篇 |
1986年 | 13篇 |
1985年 | 17篇 |
1984年 | 20篇 |
1983年 | 9篇 |
1982年 | 13篇 |
1981年 | 13篇 |
1980年 | 12篇 |
1978年 | 14篇 |
1977年 | 9篇 |
1976年 | 6篇 |
1975年 | 6篇 |
1974年 | 13篇 |
1972年 | 5篇 |
排序方式: 共有6575条查询结果,搜索用时 62 毫秒
941.
Muramatsu T Iida T Hirasawa T Kikuchi K Yasui I Murakami M Yamashita E Yasuda S Kajiwara H Yasuda M Osamura RY Mikami M 《The Tokai journal of experimental and clinical medicine》2006,31(3):87-90
We performed targeted molecular therapy in a patient with a non-resectable pelvic gastrointestinal stromal tumor (GIST). Imatinib mesylate was administered at 400-600 mg/day for 6 months, and the tumor became resectable. The patient was a 58-year-old female who visited a gynecologic hospital with the chief complaint of a swollen feeling in the lower abdomen. A pelvic tumor was found by imaging, and the patient was referred to our hospital. Laparotomy was performed, but it was found that the tumor arose from the intestinal serous membrane, rather than from the uterus, and complete excision was difficult. A portion of the tumor tissue was excised, and the abdomen was closed. GIST was diagnosed on postoperative pathological examination, and the tissue was positive for c-kit protein on immunostaining. The tumor had markedly shrunk after oral administration of imatinib mesylate for 6 months, and excision by laparotomy became possible. 相似文献
942.
Orito K Hashida M Hirata K Kurokawa A Shirai M Akahori F 《Drug and chemical toxicology》2006,29(1):1-9
Chlorhexidine gluconate (CHX) is an antiseptic that has been widely used for disinfection of cutaneous wound and gingivae. Recently, a patient who inhaled CHX solution died from acute respiratory distress syndrome (ARDS). Although it is highly possible that direct pulmonary damage might be the cause of ARDS, there is no preclinical information about the pulmonary toxicity of CHX. In the current study, the acute direct action of CHX to the lung was evaluated in rats. We successfully exposed the left but not the right lung either to CHX at concentrations of 1%, 0.1%, and 0.01% or to saline using a curved-tip administration tube. At the higher concentrations of CHX (0.1% and 1%), severe congestion to the alveoli and capillaries and perivascular and intra-alveolar hemorrhages were observed 1 day after exposure. Aniline blue-stained collagen fibers with an infiltration of inflammatory cells were present 7 days after exposure. The fibrotic changes and intra-alveolar inflammatory cells had decreased but were still observed sporadically 28 and 84 days after exposure. These detrimental effects were more severe at 1% than at 0.1% CHX. No remarkable effect was observed after exposures to 0.01% CHX and saline. We were able to evaluate the time-course changes in the pulmonary toxicity of CHX by exposures limited to the left lung. It is highly possible that CHX at a concentration of more than 0.1% might directly induce ARDS when aspirated and reaching to the alveoli. 相似文献
943.
944.
Shigeru Fujimoto Makoto Takahashi Tomohito Minami Hiroaki Ishigami Masaru Miyazaki Kenjiro Itoh 《Surgery today》1979,9(3):190-196
A prospective clinical trial was undertaken in 121 patients with stomach cancer to compare immunochemotherapy with 5-fluorouracil
and FT-207 combined with OK-432 or PS-K, immunostimulators, and plain chemotherapy with 5-fluororacil and FT-207. Of the 121
patients who received immunochemotherapy, 67 patients (group A) had undergone curative removal of the tumor. The other 54
patients had undergone non-curative tumor removal or had recurrence after non-curative tumor removal and they were divided
into two groups (groups B and C) on the basis of lymphocyte reactivity induced with PHA. Although group A exhibited a significant
increase in PHA-induced lymphocyte transformation and a trifling increase in lymphocyte counts, its survival rate within a
36 month period did not differ from that of the peer controls. Group B, composed of 21 patients showing improvement of PHA-induced
lymphocyte transformation, significantly prolonged its survival compared to the peer controls. The survival of group C, composed
of 33 patients showing a gradual drop in PHA-induced lymphocyte transformation, was not prolonged compared to the peer control
patients; and they showed significant decreases in lymphocyte counts. The overall survival of group B and group C was not
superior to that of the 48 peer controls. 相似文献
945.
Takeuchi M Keino H Suzuki J Usui Y Hattori T Takeuchi A Oh-I K Okunuki Y Kezuka T Usui M 《Experimental eye research》2006,83(4):981-988
Murine macrophages treated with TGF-beta2 are capable of inducing anterior chamber-associated immune deviation (ACAID), and these macrophages are characterized by impaired IL-12 production and CD40 expression, consequently failing to promote Th1 cell differentiation. In this study, we investigated whether human monocytes can also acquire the specific functions by TGF-beta2 treatment, even when the monocytes are isolated from patients with Behcet's disease (BD). Adherent monocytes isolated from peripheral blood mononuclear cells (PBMC) of 16 BD patients and 16 healthy controls, were cultured overnight with or without 5 ng/ml of TGF-beta2. Then, TGF-beta2-treated or untreated adherent cells were co-cultured with allogeneic CD4(+) T cells obtained from healthy subjects. TGF-beta2 treatment inhibited the abilities of adherent monocytes obtained from BD patients to stimulate the proliferation and IFN-gamma production of allogeneic CD4(+) T cells. The reduced IFN-gamma production was also confirmed by IFN-gamma mRNA expression in the co-cultured T cells. IL-12 production and CD40 molecule expression by adherent monocytes obtained from BD patients were strikingly reduced by TGF-beta2 treatment. These results suggest a possibility that adherent monocytes isolated from BD patients may acquire a property to induce ACAID by treatment with TGF-beta2. 相似文献
946.
Methylation status of genes upregulated by demethylating agent 5-aza-2'-deoxycytidine in hepatocellular carcinoma 总被引:5,自引:0,他引:5
Hirasawa Y Arai M Imazeki F Tada M Mikata R Fukai K Miyazaki M Ochiai T Saisho H Yokosuka O 《Oncology》2006,71(1-2):77-85
BACKGROUND/AIMS: To determine the clinical significance of gene promoter methylation in hepatocellular carcinoma (HCC), we examined in clinical samples the methylation status of those promoters that showed elevated activity in hepatoma cell lines after 5-aza-2'-deoxycytidine treatment. METHODS: Regarding the genes with promoter hypermethylation in the cell lines, their expression levels and methylation status in HCC and non-HCC tissues were assessed by semiquantitive RT-PCR and methylation-specific PCR. To confirm the result, the expression levels and methylation status in 16 additional HCC and non-HCC tissues were assessed. RESULTS: The promoter regions of caveolin 1 (CAV1), cysteine and glycine-rich protein 1 (CSRP1), Kruppel-like factor 6 (KLF6), myosin (light polypeptide 9) (MYL9), and transgelin (TAGLN) were highly methylated in the cell lines. CAV1 and CSRP1 were methylated in HCC more frequently than in non-HCC. KLF6, MYL9, and TAGLN were fully methylated in both HCC and non-HCC. Using additional clinical samples, downregulation of CAV1 and CSRP1 was observed in 38 and 56%, respectively, of the 16 HCC samples and aberrant methylation of CAV1 and CSRP1 was observed in 56% of HCC in both cases. CONCLUSION: CAV1 and CSRP1 were inactivated in HCC by aberrant methylation and they may serve as important biomarkers of malignancy. 相似文献
947.
Iwasaki Y Yamato H Nii-Kono T Fujieda A Uchida M Hosokawa A Motojima M Fukagawa M 《Journal of bone and mineral metabolism》2006,24(2):172-175
Patients with end-stage renal disease (ESRD) develop various kinds of abnormalities in bone and mineral metabolism, widely
known as renal osteodystrophy (ROD). Although the pathogenesis of ESRD may be similar in many patients, the response of the
bone varies widely, ranging from high to low turnover. ROD is classified into several types, depending on the status of bone
turnover, by histomorphometric analysis using bone biopsy samples [1,2]. In the mild type, bone metabolism is closest to that
of persons with normal renal function. In osteitis fibrosa, bone turnover is abnormally activated. This is a condition of
high-turnover bone. A portion of the calcified bone loses its lamellar structure and appears as woven bone. In the cortical
bone also, bone resorption by osteoclasts is active, and a general picture of bone marrow tissue infiltration and the formation
of cancellous bone can be observed. In osteomalacia, the bone surface is covered with uncalcified osteoid. This condition
is induced by aluminum accumulation or vitamin D deficiency. The mixed type possesses characteristics of both osteitis fibrosa
and osteomalacia. The bone turnover is so markedly accelerated that calcification of the osteoid cannot keep pace. In the
adynamic bone type, bone resorption and bone formation are both lowered. While bone turnover is decreased, there is little
osteoid. The existence of these various types probably accounts for the diversity in degree of renal impairment, serum parathyroid
hormone (PTH) level, and serum vitamin D level in patients with ROD. However, all patients share a common factor, i.e., the
presence of a uremic condition. 相似文献
948.
Fujinaga S Kaneko K Ohtomo Y Murakami H Takemoto M Takada M Shimizu T Yamashiro Y 《Pediatric nephrology (Berlin, Germany)》2006,21(2):277-280
Thin basement membrane nephropathy (TBMN) is characterized clinically by persistent hematuria, minimal proteinuria, normal renal function, another family member with hematuria, and a benign course. Especially in childhood TBMN, proteinuria of any degree is reported to be uncommon. We report on a boy with benign familial hematuria found by urinary screening at 3 years of age who presented with nephrotic syndrome (NS) at 15 years of age. His renal histology showed TBMN associated with minimal change disease (MCD). Treatment with corticosteroid resulted in complete remission of NS in a short period of time, while isolated hematuria persisted during the follow-up period despite this therapy. We speculate, therefore, that the nephrotic range proteinuria is not due to TBMN but rather is the manifestation of associated MCD. Several cases of TBMN with NS have been reported in adults, but it has not yet been reported in children in the literature. To our knowledge, this is the first case of childhood TBMN associated with NS resulting from coincidental MCD. 相似文献
949.
Intraperitoneal Rupture of the Ureter as a Cause of Generalized Peritonitis: Report of a Case 总被引:1,自引:0,他引:1
Osako T Kounosu H Yamamoto T Fujiwara I Sawabe Y Mori M Ito H Takada H Shirakata S 《Surgery today》2006,36(9):839-842
We report a rare case of generalized peritonitis caused by nontraumatic, intraperitoneal rupture of the ureter. An 80-year-old
woman with a history of bilateral vesicoureteral reflux and long-term urethral indwelling catheter drainage presented with
a very distended abdomen. Computed tomography showed massive ascites and intraperitoneal free gas. We performed an emergency
laparotomy, assuming a gastrointestinal perforation; but could not find a cause of generalized peritonitis. Postoperatively,
she presented with anuria and massive peritoneal drainage. The findings of a cystogram confirmed intraperitoneal ureteral
rupture. She was managed successfully with ureteral stenting. The diagnosis of this condition requires a high degree of clinical
suspicion, along with radiographic evidence and peritoneal fluid analysis. Image-guided interventions play a crucial role
in the management of ureteral urine leaks after a correct diagnosis has been made. 相似文献
950.