全文获取类型
收费全文 | 16306篇 |
免费 | 790篇 |
国内免费 | 152篇 |
专业分类
耳鼻咽喉 | 169篇 |
儿科学 | 412篇 |
妇产科学 | 137篇 |
基础医学 | 1943篇 |
口腔科学 | 391篇 |
临床医学 | 1003篇 |
内科学 | 4524篇 |
皮肤病学 | 295篇 |
神经病学 | 1034篇 |
特种医学 | 744篇 |
外科学 | 2729篇 |
综合类 | 54篇 |
预防医学 | 364篇 |
眼科学 | 189篇 |
药学 | 988篇 |
中国医学 | 29篇 |
肿瘤学 | 2243篇 |
出版年
2024年 | 13篇 |
2023年 | 136篇 |
2022年 | 250篇 |
2021年 | 496篇 |
2020年 | 272篇 |
2019年 | 319篇 |
2018年 | 427篇 |
2017年 | 331篇 |
2016年 | 410篇 |
2015年 | 418篇 |
2014年 | 508篇 |
2013年 | 692篇 |
2012年 | 1003篇 |
2011年 | 1164篇 |
2010年 | 629篇 |
2009年 | 536篇 |
2008年 | 985篇 |
2007年 | 1141篇 |
2006年 | 1029篇 |
2005年 | 1066篇 |
2004年 | 1067篇 |
2003年 | 982篇 |
2002年 | 997篇 |
2001年 | 173篇 |
2000年 | 153篇 |
1999年 | 175篇 |
1998年 | 259篇 |
1997年 | 191篇 |
1996年 | 175篇 |
1995年 | 164篇 |
1994年 | 162篇 |
1993年 | 163篇 |
1992年 | 87篇 |
1991年 | 96篇 |
1990年 | 73篇 |
1989年 | 71篇 |
1988年 | 51篇 |
1987年 | 45篇 |
1986年 | 32篇 |
1985年 | 30篇 |
1984年 | 30篇 |
1983年 | 28篇 |
1982年 | 42篇 |
1981年 | 30篇 |
1980年 | 29篇 |
1979年 | 12篇 |
1978年 | 22篇 |
1977年 | 12篇 |
1976年 | 15篇 |
1974年 | 16篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
61.
Takahashi HK Morichika T Iwagaki H Tamura R Kubo S Yoshino T Mori S Akagi T Tanaka N Nishibori M 《Clinical immunology (Orlando, Fla.)》2003,108(3):274-281
Lipopolysaccharide (LPS) binds to LPS-binding protein (LBP) in plasma and is delivered to the cell surface receptor CD14 on human monocyte. LPS is transferred to the transmembrane signaling receptor toll-like receptor (TLR) 4. In the present study, the effect of histamine on the expression of CD14 on human monocytes was investigated. Histamine concentration- and time-dependently decreased the expression of cell surface CD14, whereas histamine did not decrease mRNA for CD14 nor increase soluble CD14 (sCD14). The inhibitory effects of histamine on CD14 expression were antagonized by H2-receptor antagonist, but not by H1 and H3/H4 antagonist. The effects of selective H2-receptor agonists, 4-methylhistamine and dimaprit, on CD14 expression mimicked that of histamine indicating that histamine regulated CD14 expression through the stimulation of H2-receptors. The pretreatment with histamine partially inhibited the LPS-induced TNF-alpha production in human peripheral blood mononuclear cells (PBMC). Such inhibition might be due to the down-regulation of CD14 expression on monocytes by histamine. 相似文献
62.
63.
The volume-activated chloride current in human endothelial cells depends on intracellular ATP 总被引:6,自引:0,他引:6
Masahiro Oike Guy Droogmans Bernd Nilius 《Pflügers Archiv : European journal of physiology》1994,427(1-2):184-186
We have studied the effect of intracellular ATP on volume-activated Cl–-currents in endothelial cells from human umbilical veins by means of the whole-cell patch clamp technique. The run-down of this current in ruptured patches during repetitive applications of hypotonic solutions (HTS) could be significantly reduced if the cells were internally perfused with a pipette solution that contained 4 mmol/l ATP. This run-down was much less pronounced if currents were recorded using nystatin-perforated patches. The amplitude of the current was drastically reduced and its activation became slower if the cells were superfused with a glucose-free medium with 1 mmol/l KCN. Adding 4 mmol/l ATPS, a poorly hydrolyzable ATP-analogue, to the patch pipette prevented run-down of the current during repetitive activations by HTS, even if the cells were superfused with glucose-free solution with 1 mmol/l KCN. It is concluded that activation of the mechanosensitive Cl– conductance in human endothelial cells requires the presence of intracellular ATP, but not its hydrolysis. 相似文献
64.
Tanaka K Tamura J Kawanabe K Nawa M Uchida M Kokubo T Nakamura T 《Journal of biomedical materials research. Part A》2003,67(1):200-207
Recently zirconia/alumina composites have been examined by many researchers as the new generation of bearing materials in total joint replacements. In this study, the phase stability of a Ce-TZP/Al(2)O(3) nanocomposite and conventional Y-TZP after aging, and its influence on wear resistance, were investigated. Very slight phase transformation was observed in both types of ceramics 18 months after the implantation of Ce-TZP/Al(2)O(3) or Y-TZP samples into rabbit tibiae. However, Y-TZP showed marked phase transformation (approximately 80%) after aging in an autoclave (121 degrees C) for 190 h or in physiological saline at 62 degrees C for 18 months, whereas the new composite remained almost resistant to degradation. According to the results of self-pairing pin-on-disk wear tests using ceramic specimens with or without autoclave aging, the wear factor was almost the same between Ce-TZP/Al(2)O(3) samples with and without aging (6.74 +/- 0.36 x 10(-8) and 6.04 +/- 0.95 x 10(-8) mm(3)/Nm, respectively). In contrast, although non-aged Y-TZP had the lowest wear factor (4.88 +/- 0.51 x 10(-8) mm(3)/Nm) of all specimens tested, aged Y-TZP showed 10-fold greater wear than nonaged Y-TZP. The present study suggests that Ce-TZP/Al(2)O(3) nanocomposite has much greater phase stability than Y-TZP, and that its wear properties are not influenced by aging. 相似文献
65.
Tadakazu Shimoda Shokan Tanoue Masahiro Ikegami Yasuhiro Fujii Tetsuya Muroya Eisei Ishikawa 《Pathology international》1983,33(6):1259-1267
The present study includes a histopathological and immunohistochemical study of 4 cases of diffuse hyperplasia of gastric argyrophil cells. The mode of proliferation of these cells and the production of hormone by these cells have been documented. The distribution of microacinar nests composed of argyrophil cells was thought to be related to chronic gastritis in which there are atrophy of mucosa and intestinal metaplasia. In the case in which these nests were found only in the corpus ventriculi, there was intestinal metaplasia throughout the stomach. On the other hand, in the case in which these nests appeared only in the pyloric area, atrophy of the mucosa with mild intestinal metaplasia was observed only in the pyloric area. The microacinar nests composed of argyrophil cells were distributed in the deep mucosa at the basal portion of the glands in the area with intestinal metaplasia. Serial sections revealed a sprout composed of argyrophil cells budding from the gland with intestinal metaplastic changes. The sprout buds out from the growth zone of glands with Intestinal metaplasia and then becomes isolated and gives rise to reactive hyperplasia. The peptide hormone contained in these cells differs according to the mucosal environments. Cells containing gastrin were observed in the pyloric area, but not in the corpus ventriculi where there was marked intestinal metaplasia. The cells in this area were assumed to contain other hormones. 相似文献
66.
Masao Yoshikawa Hiroiyuki Nomori Masahiro Hatano 《Macromolecular chemistry and physics.》1978,179(10):2397-2405
Circular dichroism (CD) and ultraviolet absorption (UV) spectra were measured for poly{γ-[2-(9-carbazolyl)ethyl] L -glutamate} (PCLG), whose side chain chromophore has three π – π* transitions in a wavelength region not overlapping with the n – π* transition of the main chain peptide group. For the dilute solution in any given helicogenic solvent, the observed CD sign had a good correlation to the polarization direction of the π – π* transitions. On the other hand, a concentrated solution with a concentration of ca. 15 wt.-% was found to form a liquid crystalline phase, which was probably nematic at room temperature. The CD spectral profiles of the cast film of the polypeptide were quite different from that of the dilute solution. Origins of the optical activity are discussed. 相似文献
67.
68.
Masahiro Yamasaki 《Developmental dynamics》1990,188(3):249-259
The thyroid and thymic arteries were investigated in 50 male and 50 female rats. In more than 70% of the animals, on both sides the cranial thyroid artery forms a common trunk with the ascending pharyngeal artery. The caudal thyroid artery arises not from the deep cervical but from the pericardiacophrenic artery. It may be replaced, however, by a branch of some other artery, such as the brachiocephalic, subclavian, vertebral, or ascending cervical, suggesting a shift of its origin from the internal thoracic artery to the thyrocervical trunk as in man. All the thoracic lobes of the thymus are supplied directly by a thymic branch of the internal thoracic artery or indirectly by a branch of the pericardiacophrenic artery. More than half of the specimens have a cervical thymic lobe of variable size, which is supplied by a branch of the cranial thyroid, external carotid, and/or occipital arteries. Some of these thymic arteries, except those from the external carotid and occipital arteries, reach the thoracic lobe. The thoracic lobes lacking a cervical lobe may be supplied by the thymic branch arising only from the cranial thyroid artery. Other anomalous arteries supplying the thoracic lobe are derived from the superficial cervical and/or the right common carotid arteries. These results show that the thymic arteries of rats are basically similar to those of man, although they display a clear difference in their frequency and origin. 相似文献
69.
70.
Kawada K Yonei T Ueoka H Kiura K Tabata M Takigawa N Harada M Tanimoto M 《Acta medica Okayama》2002,56(3):129-134
When the development of chemotherapeutic agents reaches the clinical trial stage, it is necessary to perform drug sensitivity tests quickly in order to select the most promising agents for the treatment of cancer. In order to assess the possibility of using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay as a substitute for the human tumor clonogenic assay (HTCA), we evaluated the correlation between the results obtained by these 2 assays in 5 human lung cancer cell lines. The correlation coefficient between the results of the HTCA and the MTT assay was 0.673, indicating a relatively good correlation. The correlation was most prominent in platinum analogues (r = 0.939) and good in anthracyclines/anthracenedione (r = 0.611). However, no significant correlation was observed in vinca alkaloids, etoposide, irinotecan, SN-38 (an active metabolite of irinotecan), and rhizoxin. The results of the MTT assay showed a high degree of correlation with those of the HTCA in predicting the sensitivity of cancer cell lines to platinum analogues, and anthracyclines/anthracenedione. These results suggest that the MTT assay may be more convenient and quickly performed than the HTCA and can replace HTCA in evaluating the effects of anticancer agents, especially the platinum analogues and anthracyclines/anthracenedione. 相似文献