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排序方式: 共有9087条查询结果,搜索用时 31 毫秒
971.
Investigating nasal cytology as a potential tool for diagnosing occupational rhinitis in woodworkers
Claudia Staffieri MD PhD Andrea Lovato MD Federica Aielli MD Martina Bortoletto MD Luciano Giacomelli BD Mariella Carrieri MD Salvatore Romeo MD PhD Paolo Boscolo-Rizzo MD Maria Cristina Da Mosto MD Giovanni Battista Bartolucci MD Gino Marioni MD Maria Luisa Scapellato MD 《International forum of allergy & rhinology》2015,5(9):814-819
972.
Donor‐specific alloreactive T cells can be quantified from whole blood,and may predict cellular rejection after renal transplantation
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![点击此处可从《European journal of immunology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Michaela Fischer Sarah Leyking Marco Schäfer Julia Elsäßer Martin Janssen Janine Mihm Danilo Fliser Martina Sester Urban Sester 《European journal of immunology》2017,47(7):1220-1231
Preformed cellular alloreactivity can exist prior to transplantation and may contribute to rejection. Here, we used a rapid flow‐cytometric whole‐blood assay to characterize the extent of alloreactive T cells among 1491 stimulatory reactions from 61 renal transplant candidates and 75 controls. The role of preformed donor‐specific alloreactive T cells in cellular rejection was prospectively analyzed in 21 renal transplant recipients. Alloreactive CD8+ T cells were more frequent than respective CD4+ T cells, and these levels were stable over time. CD8+ T cells were effector‐memory T cells largely negative for expression of CD27, CD62L, and CCR7, and were susceptible to steroid and calcineurin inhibitor inhibition. Alloreactivity was more frequent in samples with higher number of HLA mismatches. Moreover, the percentage of individuals with alloreactive T cells was higher in transplant candidates than in controls. Among transplant candidates, 5/61 exhibited alloreactive CD8+ T cells against most stimulators, 23/61 toward a limited number of stimulators, and 33/61 did not show any alloreactivity. Among 21 renal transplant recipients followed prospectively, one had donor‐specific preformed T‐cell alloreactivity. She was the only patient who developed cellular rejection posttransplantation. In conclusion, donor‐specific alloreactive T cells may be rapidly quantified from whole blood, and may predict cellular rejection after transplantation. 相似文献
973.
Massimo Pifferi MD PhD Andrew Bush MD FRCP FRCPCH Angela Michelucci PhD Maria Di Cicco MD Martina Piras Biol Sc Davide Caramella MD Federica Mazzei MD Maria Neri MD Giovanni Pioggia PhD Gennaro Tartarisco PhD Giuseppe Saggese MD Paolo Simi Biol Sc Attilio L Boner MD 《Pediatric pulmonology》2015,50(2):179-186
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976.
Róbert Szabó Lukáš Radosa Martina Ličková Monika Sláviková Marta Heroldová Michal Stanko Milan Pejčoch Anja Osterberg Lies Laenen Susanne Schex Rainer G. Ulrich Sandra Essbauer Piet Maes Boris Klempa 《Virus genes》2017,53(6):913-917
Puumala virus (PUUV), carried by bank voles (Myodes glareolus), is the medically most important hantavirus in Central and Western Europe. In this study, a total of 523 bank voles (408 from Germany, 72 from Slovakia, and 43 from Czech Republic) collected between the years 2007–2012 were analyzed for the presence of hantavirus RNA. Partial PUUV genome segment sequences were obtained from 51 voles. Phylogenetic analyses of all three genome segments showed that the newfound strains cluster with other Central and Western European PUUV strains. The new sequences from ?umava (Bohemian Forest), Czech Republic, are most closely related to the strains from the neighboring Bavarian Forest, a known hantavirus disease outbreak region. Interestingly, the Slovak strains clustered with the sequences from Bohemian and Bavarian Forests only in the M but not S segment analyses. This well-supported topological incongruence suggests a segment reassortment event or, as we analyzed only partial sequences, homologous recombination. Our data highlight the necessity of sequencing all three hantavirus genome segments and of a broader bank vole screening not only in recognized endemic foci but also in regions with no reported human hantavirus disease cases. 相似文献
977.
Anne Hilgendorff Anita Windhorst Manuel Klein Svetlin Tchatalbachev Christine Windemuth-Kieselbach Joachim Kreuder Matthias Heckmann Anna Gkatzoflia Harald Ehrhardt Josef Mysliwietz Michael Maier Benjamin Izar Andre Billion Ludwig Gortner Trinad Chakraborty Hamid Hossain 《Journal of molecular medicine (Berlin, Germany)》2017,95(2):169-180
978.
The historical spread of Arabian Pastoralists to the eastern African Sahel evidenced by the lactase persistence −13,915*G allele and mitochondrial DNA
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![点击此处可从《American journal of human biology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
979.
980.
Shivanand Hegde Shrilakshmi Hegde Martina Zimmermann Martina Fl?ck Joachim Spergser Renate Rosengarten Rohini Chopra-Dewasthaly 《Infection and immunity》2015,83(7):2751-2761
Mycoplasmas possess complex pathogenicity determinants that are largely unknown at the molecular level. Mycoplasma agalactiae serves as a useful model to study the molecular basis of mycoplasma pathogenicity. The generation and in vivo screening of a transposon mutant library of M. agalactiae were employed to unravel its host colonization factors. Tn4001mod mutants were sequenced using a novel sequencing method, and functionally heterogeneous pools containing 15 to 19 selected mutants were screened simultaneously through two successive cycles of sheep intramammary infections. A PCR-based negative selection method was employed to identify mutants that failed to colonize the udders and draining lymph nodes in the animals. A total of 14 different mutants found to be absent from ≥95% of samples were identified and subsequently verified via a second round of stringent confirmatory screening where 100% absence was considered attenuation. Using this criterion, seven mutants with insertions in genes MAG1050, MAG2540, MAG3390, uhpT, eutD, adhT, and MAG4460 were not recovered from any of the infected animals. Among the attenuated mutants, many contain disruptions in hypothetical genes, implying their previously unknown role in M. agalactiae pathogenicity. These data indicate the putative role of functionally different genes, including hypothetical ones, in the pathogenesis of M. agalactiae. Defining the precise functions of the identified genes is anticipated to increase our understanding of M. agalactiae infections and to develop successful intervention strategies against it. 相似文献