Summary We present evidence that actin is necessary for the successful assembly of HaNPV virions. Purified nucleocapsid protein Ha-VP39 of Heliothis armigera nuclear polyhedrosis virus (HaNPV) was found to be able to bind to actin in vitro without assistance, as demonstrated by Western blot and isothermal titration calorimeter. H and binding constants (K) detected by isothermal titration calorimeter strongly suggested that Ha-VP39 first binds actin to seed the formation of hexamer complex of actin, and the hexamers then link to each other to form filaments, and the filaments finally twist into cable structures. The proliferation of HaNPV was completely inhibited in Hz-AM1 cells cultivated in the medium containing 0.5µg/ml cytochalasin D (CD) to prevent polymerization of actin, while its yield was reduced to 10–4 in the presence of 0.1µg/ml CD. Actin concentration and the viral DNA synthesis were not significantly affected by CD even though the progeny virions assembled in the CD treated cells were morphologically different from normal ones and resulted in fewer plaques in plaque assayThe authors equally contributed to the work.The authors equally contributed to the work. 相似文献
In order to evaluate the significance of the
sinus node function test by transvenous atrial
pacing in the diagno,sis of sick sinus syndrome
(SSS), sinus node recovery time (SRT), co]rrected
sinus node recovery time (CSRT) and total atrio-
sinus and sino-atrial conduction time (SACT)
are observed in 69 patients including non-SSS
group 39 cases and SSS group 30 cases.
According to our data, we suggest criteria
for sinus node dysfunction as follows. SRT>1,400
ms and/or A-V junctional esca.pe before sinus
recovery and;or secondary pause, CSRT>560 ms.
total SACT>300 ms. With l item abnormal, the
false positive rat.e in the non-SSS group is 7.7c7。
and the false negative rate in the S.SS group 3.30i"
with 2 items abnormal, the false positive rate in
the non-SSS group Oi and the false negative
rate in the SSS grOiup 6.7'70.
Atropine test results are compared with that
of atrial pacing, 95.8To and 84.2% are compatible
with each other in these 2 groups. In order to
cut the use of invasive technic to the minimum,
we propose performing the atropine test first.
Only in those with unexplainable test results
should the trial pacing test. be resorted to.. 相似文献
The benefit of transurethral laser prostatectomy over open simple prostatectomy (OSP) is controversial in aged symptomatic benign prostatic hyperplasia (BPH) patients with large volume prostates, and the aim of this study is to compare the safety and efficiency of these two methods. Meta-analysis was applied using the Review Manager V5.3 software and the retrieved randomized controlled clinical trials (RCTs) comparing transurethral laser prostatectomy with OSP were analyzed for the treatment of large volume prostates from 2000 to 2019 in PubMed, Web of Science, Cochrane, and EMBASE datasets. Five RCTs assessing transurethral laser prostatectomy versus OSP were considered suitable for this meta-analysis, which included a total of 448 patients, with 232 patients undergoing laser and 216 patients undergoing OSP. Compared with OSP, although transurethral laser prostatectomy required a longer operative time (weighted mean difference (WMD) 27.49 mins; 95% confidence interval (CI) 16.54–38.44; P?<?0.00001) and obtained a less resected prostate weight (WMD ??11.72 g; 95% CI ??21.75 to ??1.70; P?=?0.02), patients undergoing laser prostatectomy benefited from significantly less hemoglobin decline (??0.97 g/dL; 95% CI ??1.31 to ??0.64; P?<?0.00001), shorter time of catheterization (WMD ??3.67 days; 95% CI ??5.60 to ??1.75; P?=?0.0002), shorter length of hospital stay (WMD ??4.75 days; 95% CI ??6.57 to ??2.93; P?<?0.00001), and less blood transfusion (odds ratio 0.10; 95% CI 0.03 to 0.35; P?=?0.0003). During postoperative follow-up, no significant difference was observed between the two groups in IPSS, QoL, Qmax, and PVR. Both transurethral laser prostatectomy and OSP are safe and effective for large prostates that require prostate resection. Taking into account of less blood loss, shorter catheterization time and hospital stay, and less blood transfusion, transurethral laser prostatectomy may be a better treatment for patients with large prostates.
