肝硬化患者肾脏血液动力学的研究

目的 研究肝硬化患者肾脏血液动力学的改变。方法 应用多普勒超声测定４２例肝硬化患者肾脏血液动力学指标。并分析这些指标与肝功能及平均动脉压之间的关系。结果 不同阶段的肝硬化患者肾内血管收缩程度有明显的差异。结论 肝硬化早期即有肾血管的收缩,肝功越差肾血管收缩越明显。肾血管的收缩是全身血液动力学改变的一部分。     

胆必清对内毒素诱导的大鼠腹腔巨噬细胞内游离钙浓度的影响

目的：探讨中药胆必清对内毒素诱导的大鼠腹腔巨噬细胞游离钙浓度的影响。方法：制备大鼠腹腔巨噬细胞并在体外进行孵育,使用荧光探针用荧光法测定钙浓度。巨噬细胞随机分为正常对照组,内毒素LPS组,小、中和大剂量中药组。结果：LPS能诱导巨噬细胞[Ca2^ ]i的升高。加入胆必清后,[Ca2^ ]i可有不同程度的降低。结论：胆必清能抑制LPS诱导的大鼠腹腔巨噬细胞内的[Ca2^ ]i升高,提示该药具有抗炎和免疫调节作用。     

McAb共刺激PBLs淋巴细胞在培养体系中的状态及超微结构的 …

通过用抗ＣＤ３,ＣＤ２８＋ＣＤ８０ ＭｃＡｂ激活健康人的ＰＢＬｓ,并以ＰＨＡ,ＩＬ－２,ＰＢＬｓ为对照组;对各组不同时间段的淋巴细胞超微结构进行观察。结果提示：ＣＤ３及ＣＤ２８＋ＣＤ８０刺激淋巴细胞增殖外,也能使淋巴细胞活化,细胞表现为胞体增大,细胞器增多,具有粗大的绒毛和突出伪足,并可见单核细胞吞噬活跃。     

高能震波结合外敷双柏油膏治疗跟痛症

目的:探讨高能震波结合双柏油膏外敷治疗跟痛症的临床疗效.方法:采用高能震波加双柏油膏外敷治疗跟痛症32例,并与32例单纯用高能震波治疗进行对照.结果:单纯震波治疗组3周后(近期)疗效的优良率为56.25%,3个月后(远期)疗效的优良率为81.25%;而高能震波结合外敷双柏油膏组则分别为84.37%和93.75%.结果表明,高能震波结合外敷双柏油膏治疗跟痛症,其近期疗效和远期疗效均有所提高,并能减轻震波治疗所带来的血肿、骨膜剥离等副作用.     

新生儿缺氧缺血性脑病血浆及脑脊液一氧化氮水平的变化

目的 :了解新生儿缺氧缺血性脑病 (HIE)时血浆和脑脊液 (CSF)中 NO水平的动态变化。方法 :采用硝酸根还原酶法 ,对 35例 HIE患儿 (轻度 10例、中度 13例、重度 12例 )分别于急性期、恢复期进行了血浆和 CSF NO水平测定。结果 :HIE急性期血浆 NO水平除轻度组外 ,中、重度组明显高于对照组 ,重度组又明显高于轻、中度组 ;恢复期轻、中、重度组血浆 NO水平均降至正常 ,与对照组比较差异无显著性。CSF中 NO水平增加的幅度与血浆成正比。结论 :血浆和 CSF中 NO水平与 HIE的脑损伤程度密切相关     

Presynaptic inhibition of synaptic transmission in the rat hippocampus by activation of muscarinic receptors: involvement of presynaptic calcium influx

1. Modulation of presynaptic voltage-dependent calcium channels (VDCCs) by muscarinic receptors at the CA3–CA1 synapse of rat hippocampal slices was investigated by using the calcium indicator fura-2. Stimulation-evoked presynaptic calcium transients ([Ca_pre]_t) and field excitatory postsynaptic potentials (fe.p.s.ps) were simultaneously recorded. The relationship between presynaptic calcium influx and synaptic transmission was studied.
2. Activation of muscarinic receptors inhibited [Ca_pre]_t, thereby reducing synaptic transmission. Carbachol (CCh, 10 μM) inhibited [Ca_pre]_t by 35% and reduced fe.p.s.p. by 85%. The inhibition was completely antagonized by 1 μM atropine. An approximate 4^th power relationship was found between presynaptic calcium influx and postsynaptic responses.
3. Application of the N-type VDCC-blocking peptide toxin ω-conotoxin GVIA (ω-CTx GVIA, 1 μM) inhibited [Ca_pre]_t and fe.p.s.ps by 21% and 49%, respectively, while the P/Q-type VDCC blocker ω-agatoxin IVA (ω-Aga IVA, 1 μM) reduced [Ca_pre]_t and fe.p.s.ps by 35% and 85%, respectively.
4. Muscarinic receptor activation differentially inhibited distinct presynaptic VDCCs. ω-CTx GVIA-sensitive calcium channels were inhibited by muscarinic receptors, while ω-Aga IVA-sensitive channels were not. The percentage inhibition of ω-CTx GVIA-sensitive [Ca_pre]_t was about 63%.
5. Muscarinic receptors inhibited presynaptic VDCCs in a way similar to adenosine (Ad) receptors. The percentage inhibition of ω-CTx GVIA-sensitive [Ca_pre]_t by Ad (100 μM) was about 59%. There was no significant inhibition of ω-Aga IVA-sensitive channels by Ad. The inhibitions of [Ca_pre]_t by CCh and Ad were mutually occlusive.
6. These results indicate that inhibition of synaptic transmission by muscarinic receptors is mainly the consequence of a reduction of the [Ca_pre]_t due to inhibition of presynaptic VDCCs.

     

原位PCR和免疫组化检测垂体腺癌P16基因

目的研究人垂体腺瘤P16基因的改变,同时探索原位PCR技术的适宜条件和结果确认,以及用于基因缺失检测的可行性。方法原位PCR和免疫组化技术。结界绝大部分间质细胞为P16蛋白免疫组化阴性,而一部分垂体肿瘤细胞呈P16阳性;针对P16基因的原位PCR信号则可见于垂体肿瘤细胞和间质细胞等各种细胞,即P16组化阴性的细胞同样表明有P16基因存在。结论原位PCR可以是P16基因研究的一种有效手段,提示垂体腺癌的P16基因改变形式可能主要是表达过度而不是基因缺失。     

赤芍提取物的抗疱疹病毒Ⅱ型作用

为探讨赤芍提取物对Ⅱ型单纯疱疹病毒（ＨＳＶ－ Ⅱ） 的抑制作用, 用兔肾细胞作体外治疗、预防及中和实验, 对赤芍抗ＨＳＶ＿Ⅱ作用进行评价。结果： 赤芍无毒浓度为１２．５ｇ／Ｌ, 有效抑制浓度为１ ．５６ｇ／Ｌ,对ＨＳＶ＿Ⅱ的感染有抑制病毒生长和对病毒颗粒有直接杀伤作用。提示赤芍提取物确有明显的抗ＨＳＶ＿Ⅱ复制的作用