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81.
Kitano Taisuke Ito Kiyonori Ookawara Susumu Shindo Mitsutoshi Uchida Takayuki Kofuji Masaya Hayasaka Hideyuki Miyazawa Haruhisa Ueda Yuichiro Hirai Keiji Hoshino Taro Morishita Yoshiyuki 《Journal of artificial organs》2020,23(2):187-190
Journal of Artificial Organs - A 76-year-old woman on hemodialysis (HD) for diabetic nephropathy was admitted to our hospital with occasional intradialytic hypotension (IDH). We continuously... 相似文献
82.
83.
Induction of various cytokines and development of severe mucosal
inflammation by cagA gene positive Helicobacter
pylori strains 总被引:15,自引:4,他引:15 下载免费PDF全文
Background—Helicobacter pyloristrains possessing the cagA gene are thought to induceinterleukin 8 (IL-8) in gastric mucosa. However, it is still unclearwhether a relation exists between the cagA gene and theexpression patterns of cytokines other than IL-8.
Aims—To investigate therelation between the cagA gene and the productionof various cytokine proteins using an enzyme linked immunosorbentassay (ELISA).
Patients and methods—In 184 patients,the cagA gene was detected by polymerase chain reaction(PCR), and levels of production of IL-1β, IL-6, IL-7, IL-8, IL-10,and tumour necrosis factor α (TNF-α) in antral biopsy specimenswere measured by ELISA.
Results—Mucosal levels of IL-1β, IL-6,IL-8, and TNF-α were significantly higher in H pyloripositive than in H pylori negative patients.Furthermore, the mucosal levels of IL-1β and IL-8 were significantly higher in specimens infected with cagApositive strains than in those infected with cagAnegative strains. In H pylori positivepatients, the mucosal level of IL-8 was closely correlated withthat of IL-1β (p<0.0001), and the mucosal level of IL-6was closely correlated with that of TNF-α (p<0.0001).
Conclusion—These findings suggest that theability to induce cytokines differs among the strains;cagA+ strains induce various kinds ofcytokines and may cause severe inflammation, whereascagA strains induce IL-8 and IL-1β onlyweakly and may cause only mild inflammation. However, as most patientsinfected with the cagA+ strains havegastritis, these strains may not be equivalent to ulcerogenic strains.
Aims—To investigate therelation between the cagA gene and the productionof various cytokine proteins using an enzyme linked immunosorbentassay (ELISA).
Patients and methods—In 184 patients,the cagA gene was detected by polymerase chain reaction(PCR), and levels of production of IL-1β, IL-6, IL-7, IL-8, IL-10,and tumour necrosis factor α (TNF-α) in antral biopsy specimenswere measured by ELISA.
Results—Mucosal levels of IL-1β, IL-6,IL-8, and TNF-α were significantly higher in H pyloripositive than in H pylori negative patients.Furthermore, the mucosal levels of IL-1β and IL-8 were significantly higher in specimens infected with cagApositive strains than in those infected with cagAnegative strains. In H pylori positivepatients, the mucosal level of IL-8 was closely correlated withthat of IL-1β (p<0.0001), and the mucosal level of IL-6was closely correlated with that of TNF-α (p<0.0001).
Conclusion—These findings suggest that theability to induce cytokines differs among the strains;cagA+ strains induce various kinds ofcytokines and may cause severe inflammation, whereascagA strains induce IL-8 and IL-1β onlyweakly and may cause only mild inflammation. However, as most patientsinfected with the cagA+ strains havegastritis, these strains may not be equivalent to ulcerogenic strains.
Keywords:cytokines; Helicobacter pylori; cagA gene; interleukin 8; interleukin 1β
相似文献84.
Imamura S Kita M Yamaoka Y Yamamoto T Ishimaru A Konishi H Wakabayashi N Mitsufuji S Okanoue T Imanishi J 《The American journal of gastroenterology》2003,98(7):1500-1503
OBJECTIVES: The routes of human infection with Helicobacter pylori remain unclear. In the present study, we examined cockroaches as possible vectors for transmission of H. pylori. METHODS: We used a common species of cockroach (Periplaneta fuliginosa). After a 3-day fast, cockroaches were placed on agar plates containing freshly grown H. pylori (Sydney strain) (challenge group) or on sterile agar plates without H. pylori (control group). After 24 h of challenge, cockroaches were moved to disinfected containers, and sterile food and water were provided. The external surfaces (legs and body) and excreta of the cockroaches were sampled for culture, rapid urease test, and polymerase chain reaction (PCR). RESULTS: H. pylori were culturable from the excreta of the challenge group for 24 h postchallenge. Positive rapid urease test results were obtained up to day 3, and PCR analysis was positive for H. pylori DNA up to day 7 from the excreta. In contrast, H. pylori were not culturable from the external surfaces of the cockroaches. The rapid urease test was positive for only 8 h, and PCR analysis was positive for H. pylori DNA for 1 day from the external surface. CONCLUSIONS: Cockroaches usually live in unsanitary environments and may contaminate foods and food containment areas such as pantries. Transmission of H. pylori infection could be achieved via inadvertent ingestion of foods contaminated with cockroach excreta containing viable H. pylori. 相似文献
85.
Takuya Umehara Akinori Kaneguchi Takahiro Yamasaki Akihiro Matsuura Nobuhiro Kito Hideki Tanaka Kaoru Yamaoka 《Journal of Rural Medicine》2022,17(1):21
Objectives: This study examined the effects of the interaction between exercise and sleep on frailty severity in community-dwelling older adults.Materials and Methods: This was a cross-sectional study. Data were collected in July 2019. In total, 2021 adults participated who responded to a questionnaire. Among them, 672 participants (317 men and 355 women) with valid responses were included in the analysis. Ordinal logistic regression analysis was performed to examine the association between frailty severity and the interaction between exercise and sleep. The dependent variable represents three different levels of frailty. The independent variables included basic information and interaction between exercise and sleep.Results: The results of ordinal logistic regression analysis (odds ratio [OR]) showed that the period from the start of exercise (OR=0.96), age (OR=1.00 for participants in their 60 s, OR=1.65 for those in their 70s, and OR=3.13 for those aged >80 years), poor subjective health perception (OR=2.12), poor quality of sleep (OR=1.88), stress (OR=1.62), and exercise–sleep interaction (OR=1.00 based on good-exercise–good-sleep interaction, OR=3.09 poor-exercise–good-sleep interaction, and OR=3.50 poor-exercise–poor-sleep interaction) significantly contributed to the model. The Nagelkerke coefficient of determination adjusted for degrees-of-freedom (R2), which represents the contribution rate of the regression equation, was 0.334.Conclusions: Our results suggest that a combination of good exercise and good sleep is needed to prevent frailty progression in community-dwelling older adults. 相似文献
86.
87.
Yoshiaki?Shinden Akihiro?NakajoEmail author Hideo?Arima Kiyonori?Tanoue Munetsugu?Hirata Yuko?Kijima Kosei?Maemura Shoji?Natsugoe 《World journal of surgery》2017,41(6):1506-1512
Background
Intraoperative identification of the difficult-to-spot parathyroid gland is critical during surgery for thyroid and parathyroid disease. Recently, intrinsic fluorescence of the parathyroid gland was identified, and a new method was developed for intraoperative detection of the parathyroid with an original fluorescent detection apparatus. Here, we describe a method for intraoperative detection of the parathyroid using a ready-made photodynamic eye (PDE) system without any fluorescent dye or contrast agents.Methods
Seventeen patients who underwent surgical treatment for thyroid or parathyroid disease at Kagoshima University Hospital were enrolled in this study. Intrinsic fluorescence of various tissues was detected with the PDE system. Intraoperative in vivo and ex vivo intrinsic fluorescence of the parathyroid, thyroid, lymph nodes and fat tissues was measured and analyzed.Results
The parathyroid gland had a significantly higher fluorescence intensity than the other tissues, including the thyroid glands, lymph nodes and fat tissues, and we could identify them during surgery using the fluorescence-guided method. Our method could be applicable for two intraoperative clinical procedures: ex vivo tissue identification of parathyroid tissue and in vivo identification of the location of the parathyroid gland, including ectopic glands.Conclusion
The PDE system may be an easy and highly feasible method to identify the parathyroid gland during surgery.88.
L-carnitine could not improve hepatic warm ischemia-reperfusion injury despite ameliorated blood flow 总被引:4,自引:0,他引:4
Yonezawa K Tolba RH Wetter A Yamamoto Y Yamaoka Y Minor T 《The Journal of surgical research》2005,125(1):16-22
BACKGROUND: Carnitine is applied to ameliorate ischemia-reperfusion (I/R) injury of several organs. However, application to hepatic I/R injury is not frequently reported. The aim of this study was to elucidate the effect of exogenous carnitine administration to ameliorate the warm hepatic I/R injury. MATERIALS AND METHODS: Male Wistar rats were divided into two groups, a carnitine group (Car);100 mg/kg of l-carnitine administration and a control group (C); vehicle administration. Thirty minutes after administration, the left hepatic lobes were given 60-min ischemia and then reperfused. Plasma alanine aminotransferase (ALT), aspartate aminotransferase (AST), glutamate dehydrogenase (GLDH), tumor necrosis factor (TNF)-alpha, and lipoperoxides (LPO) were measured. Hepatic adenosine triphosphate (ATP) concentration was also measured. The hepatic blood flow was estimated using a Laser Doppler. The presence of apoptosis in the livers was evaluated by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining. RESULTS: In group Car, the blood flow of the left hepatic lobes was better recovered during the reperfusion period than in group C (P < 0.0001). Plasma levels of ALT, AST, GLDH, and TNF-alpha at 1 h after reperfusion were not significantly different between the groups. Although there were no statistical significances, ALT, AST, and TNF-alpha levels in group Car at 24 h after reperfusion tended to be higher than in group C. Plasma LPO levels were not different between the two groups. Also hepatic ATP concentration was not different between the two groups. TUNEL positive liver cells were visible only in group Car at 24 h after reperfusion, but not in the controls. CONCLUSIONS: Although carnitine administration improved the hepatic blood flow during the reperfusion period, we could not demonstrate a protective effect to the hepatic warm I/R injury. 相似文献
89.
Is Hepatic Resection for Large or Multinodular Hepatocellular Carcinoma Justified? Results From a Multi-Institutional Database 总被引:5,自引:4,他引:5
Ng KK Vauthey JN Pawlik TM Lauwers GY Regimbeau JM Belghiti J Ikai I Yamaoka Y Curley SA Nagorney DM Ng IO Fan ST Poon RT;International Cooperative Study Group on Hepatocellular Carcinoma 《Annals of surgical oncology》2005,12(5):364-373
Background The role of surgical resection in patients with large or multinodular hepatocellular carcinoma (HCC) remains unclear. This study evaluated the long-term outcome of patients with hepatic resection for large (>5 cm in diameter) or multinodular (more than three nodules) HCC by using a multi-institutional database.Methods The perioperative and long-term outcomes of 404 patients with small HCC (<5 cm in diameter; group 1) were compared with those of 380 patients with large or multinodular HCC (group 2). The prognostic factors in the latter group were analyzed.Results The postoperative complication rate (27% vs. 23%; P = .16) and hospital mortality rate (2.4% vs. 2.7%; P = .82) were similar between groups. The overall survival rates were significantly higher in group 1 than group 2 (1 year, 88% vs. 74%; 3 years, 76% vs. 50%; 5 years, 58% vs. 39%; P < .001). Among patients in group 2, five independent prognostic factors were identified to be associated with a worse overall survival: namely, symptomatic disease, presence of cirrhosis, multinodular tumor, microvascular tumor invasion, and positive histological margin.Conclusions Hepatic resection can be safely performed in patients with large or multinodular HCC, with an overall 5-year survival rate of 39%. Symptomatic disease, the presence of cirrhosis, a multinodular tumor, microvascular invasion, and a positive histological margin are independently associated with a less favorable survival outcome. 相似文献