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91.
Roman Dittmar Bart G. M. van Dijk Marc A. M. J. van Zandvoort Keita Ito 《Journal of orthopaedic research》2014,32(4):545-550
Regenerative medicine approaches aiming at treating degenerating intervertebral discs, a major cause of back pain, are increasingly tested in ex‐vivo disc explant models mimicking in‐vivo conditions. For assessing the efficacy of regenerative therapies, cell viability is commonly measured requiring specific labels to stain cells. Here, we demonstrate and evaluate how cellular auto‐fluorescence can be utilized to non‐invasively assess viability in disc tissue in‐situ using label‐free two‐photon microscopy. Live and dead bovine disc cells (0% and 100% cell viability) from the nucleus pulposus were seeded into collagen gels and auto‐fluorescence was characterized. Subsequently, nucleus pulposus explants were cultured for 6 days in media with different glucose supplementation (0, 0.25, 0.5, and 1 g/L) to induce different degrees of cell death. Then, samples were split and viability was assessed using label‐free two‐photon microscopy and conventional staining. Results show that live and dead nucleus pulposus cells systematically emit auto‐fluorescent light with distinct characteristics. Cell viability values obtained with label‐free microscopy did not significantly differ from those acquired with staining. In summary, monitoring auto‐fluorescence facilitates accurate cell viability assessment in nucleus tissue requiring no additional dyes. Thus, this technique may be suitable for pre‐clinical testing of regenerative therapies in nucleus pulposus cultures. © 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 32:545–550, 2014. 相似文献
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93.
Keita Itatsu Gen Sugawara Yuji Kaneoka Takehito Kato Eiji Takeuchi Michio Kanai Hiroshi Hasegawa Toshiyuki Arai Yukihiro Yokoyama Masato Nagino 《Surgery today》2014,44(7):1242-1252
Purpose
An incisional surgical site infection (I-SSI) is a frequently observed complication following colorectal surgery. Intraoperative wound management is one of the most important factors that determine the incidence of postoperative I-SSI. The purpose of this study was to assess the impact of the methods used for intraoperative wound management on the incidence of I-SSI following elective surgery for colorectal cancer.Methods
Between November 2009 and February 2011, the data of 1,980 consecutive patients who underwent elective colorectal resection for colorectal cancer were prospectively collected from 19 affiliated hospitals. The incidence of and risk factors for I-SSI were investigated.Results
Overall, 233 I-SSIs were identified (11.7 %). Forty-two possible risk factors were analyzed. Using a multivariate analysis, the independent risk factors for I-SSI were identified to be a high body mass index, previous laparotomy, chronic liver disease, wound length, contaminated wound class, creation or closure of an ostomy, right hemicolectomy procedure, the suture material used for fascial closure and the incidence of organ/space SSI.Conclusion
To prevent I-SSI following elective colorectal surgery, it is crucial to avoid making large incisions and reduce fecal contamination whenever possible. A high quality randomized control trial is necessary to confirm the definitive intraoperative procedure(s) that can minimize the incidence of I-SSI. 相似文献94.
Masakazu Yamamoto Masahiro Yoshida Junji Furuse Keiji Sano Masayuki Ohtsuka Shingo Yamashita Toru Beppu Yukio Iwashita Keita Wada Takako Eguchi Nakajima Katsunori Sakamoto Koichi Hayano Yasuhisa Mori Koji Asai Ryusei Matsuyama Teijiro Hirashita Taizo Hibi Nozomu Sakai Tsutomu Tabata Hisato Kawakami Hiroyuki Takeda Takuro Mizukami Masato Ozaka Makoto Ueno Yoichi Naito Naohiro Okano Takayuki Ueno Susumu Hijioka Satoru Shikata Tomohiko Ukai Steven Strasberg Michael G. Sarr Palepu Jagannath Tsann‐Long Hwang Ho‐Seong Han Yoo‐Seok Yoon Hee Jung Wang Shao‐Ciao Luo Ren Adam Mariano Gimenez Olivier Scatton Do‐Youn Oh Tadahiro Takada 《Journal of hepato-biliary-pancreatic sciences》2021,28(1):1-25
95.
96.
Utsunomiya T Shimada M Rikimaru T Hasegawa H Yamashita Y Hamatsu T Yamasaki M Kaku S Yamada K Sugimachi K 《Hepato-gastroenterology》2003,50(53):1609-1613
BACKGROUND/AIMS: The antioxidant and anti-inflammatory properties of sesamin (a non-fat constituent of sesame oil) have been attributed to an increased accumulation of dihomo-y-linolenic acid, a precursor of 1-series prostaglandins, and the decreasing production of proinflammatory 2-series prostaglandins and 4-series leukotrienes by inhibiting the delta-5 desaturase activity. We investigated the effects of a diet containing sesamin on hepatic ischemia-reperfusion injury in rats. METHODOLOGY: After feeding rats either a basal diet (control group) or a diet supplemented with sesamin (sesamin group) for 14 days, the rats underwent 60 minutes of partial hepatic ischemia and 3 hours of reperfusion. The phospholipid fatty acid composition of both liver and lung tissue specimens were then analyzed. The plasma levels of leukotriene B4 and PCOOH (phosphatidylcholine hydroperoxide) were also determined. RESULTS: The consumption of the dietary sesamin resulted in a significant increase in the dihomo-y-linolenic acid content in the tissue phospholipids of the liver and lung specimens. The amounts of polyunsaturated fatty acids in the lungs subjected to the ischemia-reperfusion injury were well preserved in the animals from the sesamin group. Despite a lack of differences in the levels of arachidonic acid, the plasma levels of leukotriene B4 in the rats fed dietary sesamin (88 +/- 15 pg) tended to be lower (P = 0.07) than those fed the control diet (110 +/- 20 pg). Furthermore, the plasma concentrations of PCOOH in the sesamin group (130 +/- 62 pmol) were also significantly lower (P < 0.05) than those in the control group (223 +/- 33 pmol). CONCLUSIONS: These findings indicate that a diet containing sesamin may thus reduce hepatic ischemia-reperfusion injury by inducing both antioxidant and anti-inflammatory activities. 相似文献
97.
Lu QW Morimoto S Harada K Du CK Takahashi-Yanaga F Miwa Y Sasaguri T Ohtsuki I 《Journal of molecular and cellular cardiology》2003,35(12):1421-1427
A missense mutation R141W in the strong tropomyosin-binding region of cardiac troponin T (cTnT) has recently been reported to cause dilated cardiomyopathy (DCM), following the first report of a DCM-causing deletion mutation DeltaK210. To clarify the molecular mechanism for the pathogenesis of DCM caused by this novel mutation in cTnT gene, functional analyses were made on the recombinant human cTnT mutant proteins. Exchanging human wild-type and mutant cTnTs into rabbit skinned cardiac muscle fibers revealed that R141W mutation resulted in a decrease in the Ca(2+) sensitivity of force generation, as in the case of DeltaK210 mutation lying outside the strong tropomyosin-binding region. In contrast, a missense mutation R94L in the vicinity of the strong tropomyosin-binding region associated with hypertrophic cardiomyopathy (HCM) resulted in an increase in the Ca(2+) sensitivity of force generation, as in the case of the other HCM-causing mutations in cTnT reported previously. An assay using a quartz-crystal microbalance (a very sensitive mass-measuring device) revealed that R141W mutation increased the affinity of cTnT for alpha-tropomyosin by approximately three times, whereas an HCM-causing mutation DeltaE160 in the strong tropomyosin-binding region, as well as DeltaK210 and R94L mutations, had no effects on the interaction between cTnT and alpha-tropomyosin. Since cTnT has an important role in structurally integrating cardiac troponin I (cTnI) into the thin filaments via its two-way interactions with cTnI and tropomyosin, the present results suggest that R141W mutation in the strong tropomyosin-binding region in cTnT strengthens the integrity of cTnI in the thin filament by stabilizing the interaction between cTnT and tropomyosin, which might allow cTnI to inhibit the thin filament more effectively, leading to a Ca(2+) desensitization. 相似文献
98.
Yutaka Terada Shohei Minami Keita Noguchi Hassan Y.A.H. Mahmoud Hiroshi Shimoda Masami Mochizuki Yumi Une Ken Maeda 《Emerging infectious diseases》2014,20(2):284-287
We detected ferret coronaviruses in 44 (55.7%) of 79 pet ferrets tested in Japan and classified the viruses into 2 genotypes on the basis of genotype-specific PCR. Our results show that 2 ferret coronaviruses that cause feline infectious peritonitis–like disease and epizootic catarrhal enteritis are enzootic among ferrets in Japan. 相似文献
99.
100.
Masafumi Kidoh Takeshi Nakaura Shinichi Nakamura Kazuo Awai Daisuke Utsunomiya Tomohiro Namimoto Kazunori Harada Yasuyuki Yamashita 《Heart and vessels》2014,29(2):149-155
We developed a new individually customized contrast-injection protocol for coronary computed tomography (CT) angiography based on the time-attenuation response in a test bolus, and investigated its clinical applicability. We scanned 60 patients with suspected coronary diseases using a 64-detector CT scanner, who were randomly assigned to one of two protocols. In protocol 1 (P1), we estimated the contrast dose to yield a peak aortic attenuation of 400 HU based on the time-attenuation response to a small test-bolus injection (0.3 ml/kg body weight) delivered over 9 s. Then we administered a customized contrast dose over 9 s. In protocol 2 (P2), the dose was tailored to the patient’s body weight; this group received 0.7 ml/kg body weight with an injection duration of 9 s. We compared the two protocols for dose of contrast medium, peak attenuation, variations in attenuation values of the ascending aorta, and the success rate of adequate attenuation (250–350 HU) of the coronary arteries. The contrast dose was significantly smaller in P1 than in P2 (36.9 ± 9.2 vs 43.1 ± 7.0 ml, P < 0.01). Peak aortic attenuation was significantly less under P1 than under P2 (384.1 ± 25.0 vs 413.5 ± 45.7, P < 0.01). The mean variation (standard deviation) of the attenuation values was smaller in P1 than in P2 (25.0 vs 45.7, P < 0.01). The success rate of adequate attenuation of the coronary arteries was significantly higher with P1 than with P2 (85.0 vs 65.8 %, P < 0.01). P1 facilitated a reduction in the contrast dose, reduced the individual variations in peak aortic attenuation, and achieved optimal coronary CT attenuation (250–350 HU) more frequently than P2. 相似文献