Cocaine and HIV-1 Interplay in CNS: Cellular and Molecular Mechanisms

Although antiretrovirals are the mainstay of therapy against HIV infection, neurological complications associated with the virus continue to hamper quality of life of the infected individuals. Drugs of abuse in the infected individuals further fuel the epidemic. Epidemiological studies have demonstrated that abuse of cocaine resulted in acceleration of HIV infection and the progression of NeuroAIDS. Cocaine has not only been shown to play a crucial role in promoting virus replication, but also has diverse but often deleterious effects on various cell types of the CNS. In the neuronal system, cocaine exposure results in neuronal toxicity and also potentiates gp120-induced neurotoxicity. In the astroglia and microglia, cocaine exposure leads to up-regulation of pro-inflammatory mediators such as cytokines and chemokines. These in turn, can lead to neuroinflammation and transmission of toxic responses to the neurons. Additionally, cocaine exposure can also lead to leakiness of the blood-brain barrier that manifests as enhanced transmigraiton of leukocytes/monocytes into the CNS. Both in vitro and in vivo studies have provided valuable tools in exploring the role of cocaine in mediating HIV-associated neuropathogenesis. This review summarizes previous studies on the mechanism(s) underlying the interplay of cocaine and HIV as it relates to the CNS.     

Visual outcomes after small incision lenticule extraction and implantable collamer lens V4c for moderate myopia: 1-year results

Graefe's Archive for Clinical and Experimental Ophthalmology - To compare 1-year visual outcomes after implantable collamer lens V4c (EVO-ICL) implantation and small incision lenticule...     

An ionic diode based on a spontaneously formed polypyrrole-modified graphene oxide membrane

Asymmetric membranes derived from the stacking of graphene oxide (GO) nanosheets have attracted great attention for the fabrication of ionic diodes. Herein, we described an ionic diode based on a polypyrrole-modified GO membrane with a vertical asymmetry, which was achieved by a spontaneous oxidation polymerization of pyrrole monomers on one side of the GO membrane in vapor phase. This asymmetric modification resulted in an asymmetric geometry due to the occupation of the interlayer space of one side of the GO membrane by polypyrrole. Our ionic diode demonstrated an obvious ionic rectification behavior over a wide voltage range. A calculation based on Poisson–Nernst–Planck equations was used to theoretically investigate the role of asymmetric modification of polypyrrole.

We described an ionic diode based on polypyrrole-modified graphene oxide membrane, which was prepared by a spontaneous oxidation polymerization of pyrrole monomers.     

Intrinsic defect engineered Janus MoSSe sheet as a promising photocatalyst for water splitting

The Janus MoSSe sheet has aroused significant attention due to its band edge position and intrinsic dipole moment, making it a strong candidate for water splitting photocatalysis. However, weak water adsorption seriously prevents its further application. Here, first-principles calculations are used to explore the effect of intrinsic defects on water adsorption and conversion at the Janus MoSSe sheet. First-principles calculation results clearly show that intrinsic defects (S_vac, Mo_anti, and Mo_int) can effectively alter the interaction between water and the MoSSe sheet. Except for S_vac defects, the adsorption energy of water at Mo_anti or Mo_int defects can be significantly increased by −1.0 to −1.5 eV with respect to the weak water adsorption on a pristine MoSSe sheet of about −0.24 eV. More importantly, the energy barrier for water conversion can be dramatically lowered by 48% to 0.7 eV at Mo_anti or Mo_int defects, together with a more stable final state. Such significant enhancement of the adsorption energy is attributed to the red shift of water energy levels, resulting from the strong interaction between O2p orbitals and Mo3d orbitals. It is shown that the intrinsic defects have the potential to change the photocatalytic reactivity of the surface, and thus this may serve as an important way to design photocatalysts for water splitting.

The Janus MoSSe sheet has aroused significant attention due to its band edge position and intrinsic dipole moment, making it a strong candidate for water splitting photocatalysis.     

The broad-spectrum and ultra-sensitive detection of zeranol and its analogues by an enzyme-linked immunosorbent assay in cattle origin samples

Zeranol (α-zearalanol) has been used as a growth promoter in livestock since 1969 in some non-EU countries; the residues of zeranol and its five analogues in animal origin foods may endanger human health due to their strong estrogenic and anabolic activities. Therefore, it is urgent to establish simple, rapid, real-time, broad-spectrum and high-sensitivity detection methods for the residues of zeranol and its analogues. In this study, an ultrasensitive indirect-competition enzyme-linked immunosorbent assay (ic-ELISA) was established for the rapid multi-residue detection of zeranol and its five analogues in cattle origin samples, which was based on a broad-spectrum monoclonal antibody (mAb) that specifically bound to zeranol and its analogues with high sensitivity. The half maximal inhibitory concentration (IC₅₀) values for zeranol, β-zearalanol, zearalanone, α-zearalenol, β-zearalenol, and zearalenone were 0.103, 0.080, 0.161, 0.177, 0.254, and 0.194 ng mL⁻¹, respectively, the recovery rates of cattle origin samples spiked with zeranol ranged from 79.2–104.2%, and the coefficient of variation (CV) values were less than 11.4%. Excellent correlation (R² = 0.9845) was obtained between the results of HPLC-MS/MS and ic-ELISA. In conclusion, the developed ic-ELISA could be employed as an ultrasensitive and broad-spectrum detection method for monitoring trace ZEN residues in cattle origin foods.

This paper presents a broad-spectrum and ultra-sensitive ic-ELISA method for the rapid detection of zeranol and its analogues in cattle origin samples.     

A highly hydrophilic benzenesulfonic-grafted graphene oxide-based hybrid membrane for ethanol dehydration

A new type of hybrid membrane was prepared by blending sodium alginate (SA) with benzenesulfonic-grafted graphene oxide (BS@GO), which showed higher hydrophilicity and more defects or edges than GO to create channels for the transfer of water molecules. BS@GO was synthesized by reacting aryl diazonium salts with graphene oxide (GO). The BS@GO sheets were aligned parallelly to the membrane surface and affected the interactions between the SA chains. BS@GO could improve the hydrophilicity and pervaporation properties of SA-based hybrid membranes. Also, compared to GO fillers, BS@GO fillers could supply higher water permeance to improve the pervaporation flux and separation factor. For the pervaporation of 90 wt% aqueous ethanol at 343 K, the optimum hybrid membrane with 1.5 wt% BS@GO in the SA matrix showed the maximum permeate flux of 703 ± 89 g m⁻² h⁻¹ (1.4 times higher than that of an SA membrane), and the highest separation factor was 5480 ± 94 (5.6 times higher than that of the SA membrane). Moreover, the hybrid membrane exhibited good stability and separation ability during long-term testing.

A new type of hybrid membrane was prepared by blending sodium alginate with benzenesulfonic-grafted graphene oxide, which showed higher hydrophilicity and more defects or edges than GO to create channels for the transfer of water molecules.     

微电极射频电化学疗法治疗内痔60例疗效观察

为观察微电极射频电化学疗法治疗内痔的疗效,将内痔患者120例随机分为治疗组和对照组,每组60例。治疗组采用微电极射频电化学疗法治疗,对照组采用铜离子电化学疗法治疗。结果显示,两组对痔出血疗效相近（P〉0．05）,但治疗组凝固点少,治疗时间短（P〈0．05）。两组对痔脱出近期有效率相近（P〉0．05）。但治疗组术后肛管黏膜溃疡发生率少于对照组（P〈0．05）。结果表明,微电极射频电化学疗法使内痔的手术治疗由微创向无创的方向迈进了一步,其更具有实用性。