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991.
目的 评价不同剂量国产尿激酶静脉溶栓治疗急性心肌梗死 (AMI)的疗效。方法 随机抽取住院AMI患者分成两组 ,分别给予常规剂量 (12 0~ 15 0万U)和大剂量 (2 0 0~ 30 0万U)尿激酶静脉溶栓治疗 ,观察其疗效。结果 常规剂量组再通率 (5 5 5 6 % ) <大剂量组 (76 47% ) ,P <0 0 5 ;常规剂量组出血率 (5 5 6 % ) <大剂量组 (17 6 5 % ) ,P <0 0 5 ;常规剂量组 4周内病死率 (5 5 6 % ) <大剂量组 (5 88% ) ,P >0 0 5。结论 尿激酶静脉溶栓治疗AMI ,剂量增大再通率就增大 ,但出血等并发症亦增加 ,总死亡率并未降低。因此主张应用 12 0~ 15 0万U为宜。 相似文献
992.
993.
香菇多糖与IL-2合用对肺癌浸润淋巴细胞抗瘤活性的影响 总被引:1,自引:0,他引:1
目的探讨香菇多糖与IL-2合用对肿瘤浸润淋巴细胞(TIL)抗瘤活性的影响。方法将TIL分别置于含IL-2或IL-2加香菇多糖培养液中培养30d,观察TIL的扩增能力、抗瘤活性的变化。结果IL-2加香菇多糖培养的Til平均扩增倍数和抗瘤活性均明显高于IL-2培养的TIL。IL-2加香菇多糖培养的TIL对自体瘤细胞,K562和Raji细胞的最大杀伤活性分别为67.18±14.73%,71.25±15.86%和66.15±15.07%;而单纯IL-2培养的TIL对各种靶细胞的最大杀伤活性分别为43.81±12.17%,50.83±13.56%和45.41±13.76%。两组相比,差异显著(P<0.05)。结论香菇多糖与IL-2合用具有促进TIL扩增,增强其抗瘤活性的作用。 相似文献
994.
From the roasted seeds ofCassia tora L., a new naphthopyrone glycoside was isolated and characterized as 10-[(β-D-glucopyranosyl-(1→6)-O-β-D-glucopyranosyl)oxyl-5-hydroxy-8-methoxy-2-methyl-4H-naphtho
[1,2-b]pyran-4-one(isorubrofusarin gentiobioside). Along with isorubrofusarin gentiobioside, alaternin and adenosine were isolated
and identified. 相似文献
995.
The antioxidant activity ofArtemisia iwayomogi was determined by measuring the radical scavenging effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical The methanol extract
ofA. iwayomogi showed strong antioxidant activity, and thus fractionated with several solvents. The antioxidant activity potential of the
individual fraction was in the order of ethyl acetate>n-butanol>water>chloroform>n-hexane fraction. The ethyl acetate andn-butanol soluble fractions exhibiting strong antioxidant activity were further purified by repeated silica gel and Sephadex
LH-20 column chromatography. Antioxidant chlorogenic acid was isolated as one of the active principles from then-butanol fraction, together with the inactive components, 1-octacosanol, scopoletin, scopolin, apigenin 7,4′-di-O-methylether luteolin 6,3′-di-O-methylether (jaceosidin), apigenin 7-methylether (genkwanin), 2,4-dihydroxy-6-methoxyacetophenone 4-O-β-D-glucopyranoside and quebrachitol. The antioxidant activity of chlorogenic acid was comparable to that of L-ascorbic acid,
which is a well known antioxidant. 相似文献
996.
Penicillin sensor was prepared by immobilizing penicillinase (Pcase) on H+-selective carboxylated poly (vinyl chloride) (PVC-COOH) membrane or cellulose filter membrane. The immobilization techniques
are as follows. Pcase was immobilized with GTH on H+-selective PVC-COOH membrane or some amount of BSA was dropped on that membrane. Another method to make immobilization is
to mix type I Pcase with GTH and drop on a cellulose filter membrane. According to immobilization techniques, there were some
differences in response properties of enzyme electrodes, however, all electrodes responded to Pcase-resistant penicillin derivatives.
Pcase immobilized on cellulose filter membrane with H+-selective PVC membrane eletrode was more stable and more sensitive to penicillinase-resistant penicillin derivatives than
any other immobilization techniques. 相似文献
997.
The NADH reductase component of the steroid 9α-hydroxylase fromMycobacterium fortuitum was purified to homogeneity. Recovery of the enzyme from the 50≈60% ammonium sulfate saturated fraction was 49%, with a purification
factor of 100-fold. The NADH reductase has a relative molecular mass of 60 KDa as determined by SDS-PAGE. The absorption maxima
at 410 and 450 nm indicate the presence of iron-sulfur group and flavin. These prosthetic groups seemed to function as redox
groups that transfer electrons from NADH to the following protein. The KM value for NADH as substrate was 68 μM. The NH2-terminal amino acid sequence of the reductase was determined as Met-Asp-Ala-Ile-Thr-Asn-Val-Pro-Leu-Pro-Ala-Asn-Glu-Pro-Val-His-Asp-Tyr-Ala-Thr.
This sequence does not show a homology with the NH2-terminal sequences reported for the reductase component of other monooxygenases, suggesting that the NADH reductase component
of the steroid 9α-hydroxylase system is novel. 相似文献
998.
By human intestinal bacteria, saikosaponin c was transformed to four metabolites, prosaikogenin E1 (E1) prosaikogenin E2 (E2), prosaikogenin E3 (E3) and saikogenin E. Metabolic time course of saikosaponin c was as follows; in early time, saikosaponin c was converted to E1 and E2, and then these were transformed to saikogenin E via E3. Also, this metabolic pathway was similar to the metabolism of saikosaponin c by rat intestinal bacteria.Bacteroides JY-6 andBacteroides YK-4, the bacteria isolated from human intestinal bacteria, could transform saiko-saponin c to E via E1 (or E2) and E3. However, these bacteria were not able to directly transform E1 and E2 to saikogenin E. Naringin was mainly transformed to naringenin by human intestinal bacteria. The minor metabolic pathway transformed naringin to naringenin via prunin. By JY-6 or YK-4, naringin was metabolized to naringenin only via prunin. 相似文献
999.
Bioassay-guided fractionation of the aerial parts ofPilea mongolica (Urticaceae) afforded two cytotoxic triterpenoids, epi-oleanolic acid (I) and oxo-oleanolic acid (II). The structures of the compounds were confirmed by spectral and synthetic evidences. CompoundI and compoundII exhibited cytotoxicity against cultured human tumor cell lines, A549 (non small cell lung adenocarcinoma), SK-OV-3 (ovarian), SK-MEL-2 (skin melanoma), XF498 (CNS) and HCT15 (colon) with ED(50) values of 3.2 approximately 8.1 mug/ml and 0.7 approximately 6.8 mug/ml, respectively. 相似文献
1000.
In order to develop new anti-inflammatory agents having different action mechanisms compared with nonsteroidal and steroidal anti-inflammatory drugs, the culture broths of various actinomycetes isolated from soil were screened using anin vivo mouse ear edma assay and one strain (Streptomyces sp. MT 2705-4: KCTC 8651P) was selected. Activity-guided purification led to the isolation of a polyether compound, dianemycin. Topically, dianemycin showed a potent anti-inflammatory activity in mouse ear edema induced by croton-oil or arachidonic acid. ED(50) value of dianemycin was found to be 0.8 mg/ear compared to 0.4 mg/ear of prednisolone in croton-oil ear edema. However, dianemycin did not show the inhibitory activity in UV-erythema and delayed hypersensitivity reaction. These results indicate that dianemycin is a potential topical anti-inflammatory agent. 相似文献