阿霉素注射次数及剂量对肾病综合征模型的影响

目的：建立阿霉素（ADR）大鼠肾病综合征模型,探讨相关影响因素,考察阿霉素的给药剂量与造模方法（一次性注射和分次注射）,确立造模的最佳条件。方法：利用尾静脉一次性注射和分次注射阿霉素,构建大鼠肾病综合征动物模型。结果：阿霉素造模后大部分出现尿蛋白排出量增加、精神不佳等现象。B组（一次性尾静脉注射ADR7.5mg/kg）死亡率66.7%,C组（分二次尾静脉注射ADR,共7.5mg/kg）,D组（一次性尾静脉注射ADR6mg/kg）死亡率25%;F组（一次性尾静脉注射ADR5mg/kg）,尿蛋白含量相对于其他几组偏低;E组（分二次尾静脉注射ADR,共6mg/kg）大鼠精神状态良好,能较快时间达到较高的尿蛋白含量（〉250mg/24h）;病理切片显示E组出现肾小球局灶节段性硬化和节段性纤维素样坏死。结论：从精神状态、尿蛋白含量、死亡率、病理切片等综合比较,尾静脉注射ADR4mg/kg,1周后再注射2mg/kg,4周后可以高效地复制出阿霉素肾病综合征大鼠模型。     

红外热像图对乳腺癌诊断价值的探讨(附21例分析)

目的：探讨红外热像图对乳腺癌的诊断价值。方法：采用医用红外热像仪对287例临床拟诊为乳腺病患者进行了检查。常规取正前位及左右斜位扫描，并逐一测量异常病灶的温度。结果：287例患者中，共检出乳腺癌21例，均经手术及病理证实。其中浸润性乳头状导管癌8例，髓样癌4例，硬癌5例，单纯癌2例，腺癌和肉瘤各1例。结论：红外热像图对乳腺癌的早期诊断有独到之处。     

不同性别青年人体重指数对血压升高的影响研究

高血压是全球主要的公共健康问题,包括高血压在内的心血管疾病已成为我国首要的死亡原因,高血压和高血压前期是主要的危险因素,它们显著增加了心血管疾病的病死率[1-2]。体重指数(body mass index,BMI)的升高增加了高血压、脑中风和心肌梗死等心血管疾病发病的风险,BMI与血压升高所致的心血管疾病发病风险不尽相     

Heterogeneous effects of obesity on mental health: Evidence from Mexico

Obesity can spread more easily if it is not perceived negatively. This issue may be more pronounced among the poor, a conjecture that we test in this paper. We start with general evidence on the concave relationship between income and obesity, both across countries and within Mexico, a country characterized by very unequal development levels and the highest obesity rate in the world. We suggest a general model that explains this stylized fact from a simple necessary condition, namely, the complementarity between nonfood consumption and health concerns. Then, we test the direct effect of overweight on mental health among Mexican women. We find a positive effect of obesity in the low consumption group and a depressing effect among the rich. This result is robust to the inclusion of a range of confounders (childhood conditions, lifestyle variables, food expenditure, and household shocks) and after instrumenting individual fatness by the variation in genetic predisposition. The complementarity between living standards and weight concerns may reflect different norms, different labor market penalties, or simply different returns to healthy time across the social spectrum.     

Dynamics revelation of conformational changes and binding modes of heat shock protein 90 induced by inhibitor associations

Heat shock protein 90 (Hsp90) has been an attractive target of potential drug design for antitumor treatment. The current work integrates molecular dynamics (MD) simulations, calculations of binding free energy, and principal component (PC) analysis with scanning of inhibitor–residue interaction to probe the binding modes of inhibitors YK9, YKJ and YKI to Hsp90 and identify the hot spot of the inhibitor–Hsp90 binding. The results suggest that the introductions of two groups G1 and G2 into YKJ and YKI strengthen the binding ability of YKJ and YKI to Hsp90 compared to YK9. PC analysis based MD trajectories prove that inhibitor bindings exert significant effects on the conformational changes, internal dynamics and motion modes of Hsp90, especially for the helix α2 and the loops L1 and L2. The calculations of residue-based free energy decomposition and scanning of the inhibitor–Hsp90 interaction suggest that six residues L107, G108, F138, Y139, W162 and F170 construct the common hot spot of the inhibitor–residue interactions. Moreover the substitutions of the groups G1 and G2 in YKJ and YKI lead to two additional hydrogen bonding interactions and multiple hydrophobic interactions for bindings of YKJ and YKI to Hsp90. This work is also expected to contribute theoretical hints for the design of potent inhibitors toward Hsp90.

Heat shock protein 90 (Hsp90) has been an attractive target of potential drug design for antitumor treatment.     

薄层扫描法测定冠舒片中延胡索乙素含量

采用薄层扫描法测定冠舒片及原料元胡中延胡索乙素含量,方法简便,结果准确。平均回收率为９９．５０％,ＲＳＤ＝１．６７％。该方法可较好地控制产品质量     

Cytocompatibility of Three Corneal Cell Types with Amniotic Membrane

Rabbit limbal corneal epithelial cells, corneal endothelial cells and keratocytes were cultured on amniotic membrane. Phase contrast microscope examination was performed daily. Histological and scan electron microscopic examinations were carried out to observe the growth, arrangement and adhesion of cultivated cells. Results showed that three corneal cell types seeded on amniotic membrane grew well and had normal cell morphology. Cultured cells attached firmly on the surface of amniotic membrane. Corneal epithelial cells showed singular layer or stratification. Cell boundaries were formed and tightly opposed. Corneal endothelial cells showed cobblestone or polygonal morphologic characteristics that appeared uniform in size. The cellular arrangement was compact. Keratocytes elongated and showed triangle or dendritic morphology with many intercellular joints which could form networks. In conclusion, amniotic membrane has good scaffold property, diffusion effect and compatibility with corneal cells. The basement membrane side of amniotic membrane facilitated the growth of corneal epithelial cells and endothelial cells and cell junctions were tightly developed. The spongy layer of amniotic membrane facilitated the growth of keratocytes and intercellular joints were rich. Amniotic membrane is an ideal biomaterial for layering tissue engineered cornea.     

C/C复合材料表面等离子喷涂HA涂层在SBF中的试验

目的 探讨不同模拟体液对HA涂层体外生物学行为的影响。方法采用等离子体喷涂法,在碳／碳复合材料表面制备HA涂层,并对涂层进行了热处理。利用X射线衍射仪(X-ray diffraction)、扫描电子显微镜(Scanning electron microscopy)等检测手段,分析该涂层的物相和形貌,观察HA涂层在模拟体液中的影响。结果XRD检测结果表明,在模拟体液浸泡过程中,涂层的主要组成相的相对含量和结晶度不断发生变化。由SEM分析结果可知,在浸泡过程中,内送粉方式下制得的HA涂层的表面沉积物呈现近似网状的结构。同时,在Ringer溶液中,可以观察到HA涂层溶解痕迹。结论通过适当的热处理可以恢复HA的结构完整性,同时提高涂层的结晶度。     

多层螺旋CT肝门静脉成像及其临床应用价值

目的：探讨多层螺旋CT肝门静脉成像(MScTP)技术在显示肝内肝门静脉解剖及其在占位性病变定位中的应用价值。方法：对170例疑有肝脏疾病患者行增强后肝门静脉期扫描，对每一例均行三维重建后处理．对二维及三维图像进行对比分析。结果：MSCTP对肝内门静脉第5级以上分支的显示率为94．5％；170例肝内门静脉可分为3种类型；二维及三维图像对病变定位不相符合率为36．8％。结论：MSCTP可以用来准确评价肝内门静脉解剖并对肝脏病灶进行准确定位：     

人格特征与防御方式的相关研究

目的 :尝试用防御机制解释EPQ各维度的特征。方法 :采用EPQ人格问卷、DSQ防御方式问卷、16PF中的G量表对 2 2 0例成人进行测试。结果 :本样本中EPQ各维度与成熟防御方式均无显著相关 (P >0 .0 5 ) ,N维度和P维度均与不成熟防御方式、中间型防御方式有显著正相关 (P <0 .0 0 1)。结论 :本研究显示 ,可以用防御机制解释EPQ各维度的某些特征。