HNE1细胞株光动力学治疗前后形态学的动态变化

目的 探讨体外光动力学治疗（ＰＤＴ）引起鼻咽癌（ＮＰＣ）细胞死亡的作用机制。方法对鼻咽癌细胞株ＨＮＥ１体外光动力学治疗前后形态学变化进行动态观察。结果 对照组ＨＮＥ１细胞培养３ｈ,６ｈ,１２ｈ,２４ｈ和４８ｈ后细胞形态与正常培养组ＨＮＥ１细包开始变形皱缩,细胞界限模糊,细胞透光度和立体感较差,治疗２４～４８ｈ后细胞发生凋亡。结论 体外光动力学治疗对ＨＮＥ１具有明显的杀伤效应并ＨＮＥ１细胞凋亡。ＰＤ     

地尔硫Zhuo及过氧歧化酶对心肌缺血再灌注损害的保护

对比地尔硫Ｚｈｕｏ及过氧歧化酶（ＳＯＤ）对心肌缺血再灌注损害的保护作用,以及明确联合应用ＳＯＤ＋ＤＴＺ的疗效是否比单一药物更好。方法：将３７只小猪造成在体心肌缺血再灌注模,分为４组。Ⅰ组１０只于再灌注前５分 静脉注射生理盐水作为对照组,Ⅱ组９只再灌注前５分钟静脉注射ＳＯＤ,Ⅲ组８只于再灌注前５分钟静脉注射ＤＴＺ,Ⅳ组９只于再灌注前５分钟静脉注射ＤＴＺ＋ＳＯＤ,结果：（１）心梗范围;用药各组与对照组     

Polymorphism of ACE gene and its relationship with serum ACE activity in the Hans in Chengdu region]

This study was aimed at the angiotensin converting enzyme (ACE) gene insertion/deletion(I/D) polymorphism distribution in Chinese and its relationship with serum ACE activity(SACE). SACE activity and ACE gene polymorphism in 90 healthy persons in Chengdu region were investigated using a spectrophotometric assay and the PCR technique, respectively. The results showed that, in the Hans, DD genotype of ACE gene was 18%, DI genotype 44%, and II genotype 38%. Allele frequencies were 0.40 for the D allele and 0.60 for the I allele. There were significant relationships between ACE I/D polymorphism and SACE activity. The results suggest that ACE genotype distribution be different between races, that II genotype and I allele be more frequent in Chinese, and that SACE activity be under the influence of a genetic polymorphism of ACE in Chinese.     

2型糖尿病及其血管并发症患者血清ACE活性的变化

This investigation was made with reference to the changes of serum angiotensin converting enzyme (SACE) activity in type 2 diabetes and its vascular complications. SACE activity was studied in 127 type 2 diabetic patients and 90 healthy persons by using a spectrophotometric assay. The results showed SACE activity was obviously higher in diabetic patients (459.51 +/- 175.85 U) than in healthy persons (321.14 +/- 121.27 U); SACE activity was significantly higher in type 2 diabetic patients with diabetic nephropathy (548.27 +/- 166.60 U) than in patients without diabetic nephropathy (383.2 +/- 139.00 U), but there was no difference between patients with microalbuminuria and macroalbuminuria; no statistical difference was detected in SACE activity between diabetic patients with diabetic retinopathy (465.64 +/- 178.93 U) and without retinopathy (449.07 +/- 170.04 U); SACE activity was not associated with the course of diabetes, blood pressure, blood lipid and blood glucose. These data suggest that raised SACE activity might only play a role in the initiation of type 2 diabetes and diabetic nephropathy, but not relate to the progress of diabetic nephropathy, the onset of diabetic retinopathy and hypertension.     

固定化葡萄糖氧化酶流动注射化学发光法测定血糖

Luminol-hemin system was used for chemiluminescent flow-injection analysis with immobilized glucoxidase(GOD) column to determine blood glucose. The method is fast, simple and sensitive. Blood samples were diluted to 1:1000 and pumped into the flow-injection analyzer for direct detection. The sampling frequency was 30 samples/h, with a detection limit of 7.2 x 10(-9) mol/L(1.30 micrograms/L). A column packed with 36 milligrams of CPG could be used nearly 200 times. The determination results of glucose in serum by this method were in good agreement with those obtained by the routine GOD spectrophotometric method (correlation coefficient r = 0.90, P > 0.05). Recoveries of this method were 94.0%-107.2%.     

粘附分子在直肠腺癌患者淋巴管内皮细胞的表达

为探讨癌细胞淋巴管侵润转移机理,对８例人的直肠腺癌的癌周围组织和转移淋巴结以及５例正常人的直肠组织和淋巴结,采用免疫组织化学方法,检测ＩＣＡＭ－１和ＣＥＡ的表达。结果显示癌周直肠组织中的淋巴管及转移的淋巴结中的淋巴管内皮细胞均有ＩＣＡＭ－１和ＣＥＡ的表达。由此提示在癌细胞沿淋巴管转移过程中,ＩＣＡＭ－１和ＣＥＡ是介导癌细胞和淋巴管内皮细胞稳定粘附的分子。     

过氧化物诱导LO2细胞凋亡时Fas mRNA表达及Ca^2＋流变化的单 …

探讨过氧化物所致肝细胞凋亡时Ｆａｓ基因表达与Ｃａ＾２＋流变化的相互关系。方法应用全细胞膜片错单细胞逆转录聚合酶链反应技术进行Ｈ２Ｏ２诱导人类正常肝细胞ＬＯ２细胞ＦａｓｍＲＮＡ表达的单细胞分析,应用全细胞膜片错显微荧光单细胞胞浆游离钙浓度测量技术、流式细胞术、扫描电镜观察同期瞬时Ｃａ＾２＋流变化及早期凋亡（Ａｎｎｅｘｉｎ－Ｖ）细胞指数和细胞膜变化。结果Ｈ２Ｏ２作用２ｈ单个ＬＯ２细胞电泳图分析可见Ｆａ     

血管内皮生长因子165反义RNA治疗人脑胶质瘤的实验研究

目的 探讨应用血管内皮生长因子１６５（ＶＥＧＦ１６５）反义ＲＮＡ治疗人脑胶质瘤的可行性。方法 构建和鉴定反义ＶＥＧＦ１６５真核表达载体;将其转染入人脑胶质瘤细胞ＳＨＧ４４,检测其转染前、后的生物学性状;比较转染前、后ＳＨＧ４４细胞的裸鼠皮下致瘤性;分别应用免疫印迹、免疫组织化学、微血管计数、电镜和流式细胞仪检测上述改变。结果 成功构建反义ＶＥＧＦ１６５真核表达载体并在ＳＨＧ４４细胞获得表达,该细胞     

Noninvasive three-dimensional computed tomographic angiography in preoperative detection of intracranial arteriovenous malformations

Ｃｏｎｖｅｎｔｉｏｎａｌｉｎｔｒａ ａｒｔｅｒｉａｌｄｉｇｉｔａｌｓｕｂｔｒａｃｔｉｏｎａｎｇｉｏｇｒａｐｈｙ (ＤＳＡ)ｕｓｅｄｔｏｂｅｒｅｇａｒｄｅｄａｓｔｈｅｇｏｌｄｓｔａｎｄａｒｄｆｏｒｄｉａｇｎｏｓｉｓｏｆｉｎｔｒａｃｒａｎｉａｌａｒｔｅｒｉｏｖｅｎｏｕｓｍａｌｆｏｒｍａｔｉｏｎｓ(ＡＶＭｓ) Ｒｅｃｅｎｔｌｙ ,ｎｅｗｔｅｃｈｎｉｑｕｅｓｏｆｎｅｕｒｏｉｍａｇｉｎｇａｎｄｃｏｍｐｕｔｅｒａｓｓｉｓｔｅｄｔｈｒｅｅ ｄｉｍｅｎｓｉｏｎａｌｒｅｃｏｎｓｔｒｕｃｔｉｏｎｍａｋｅｉｔｐｏｓｓｉｂｌｅ…     

脑复素注射液中乙酰胆碱活力的探讨

[目的 ]测定自制的脑复素注射液中乙酰胆碱的活力 .[方法 ]用自制的乙酰胆碱传感器 ,参照阿尼生物化学检查法测定活性 .[结果 ]自制的脑复素注射液和奥地利产脑活素中的乙酰胆碱活力相当于 86 0mmol/L .[结论 ]自制的药中多肽含量高 ,而乙酰胆碱活性也高