Objectives: Gouty arthritis is caused by the deposition of monosodium urate (MSU) crystals in joints, which is associated with the rise of serum urate content. This study aims to investigate the therapeutic effect of Madecassoside on gouty arthritis and hyperuricemia.
Methods: DBA/1 mice were intradermally injected with MSU to stimulate joint inflammation or intraperitoneally injected with MSU to trigger peritonitis. Moreover, ICR mice were exposed to potassium oxonate to stimulate hyperuricemia.
Results: Madecassoside repressed MSU-triggered pad swelling, joint 99mTc uptake, and joint inflammation in DBA/1 mice with gouty arthritis. Neutrophil infiltration and IL-1β & IL-6 & MCP-1 secretion was also alleviated in lavage fluids from DBA/1 mice with peritonitis due to Madecassoside treatment. Furthermore, Madecassoside decreased MSU-induced neutrophil cytosolic factor 1, caspase-1 and NLRP3 expression in mice with peritoneal inflammation. In hyperuricemic mice, Madecassoside improved renal dysfunction. Serum uric acid, BUN, and creatinine were down-regulated by Madecassoside.
Conclusion: These findings indicate that Madecassoside has potential to ameliorate inflammation in both acute gouty arthritis model and peritonitis model, probably via regulating IL-1β and NLRP3 expression.
Practical point: Madecassoside also exhibited a urate-lowering effect and a renal protective effect in hyperuricemic mice. 相似文献
Mouse and rat pathological models were pro
duced by injections of thyroid hormone (T3 0r T4,
Model. I) or hydrocortisone (HC, Model II). A
single subcutaneous dose of isoproterenol (ISO)
caused plasma cAMP to peak in 5-15 min which
was significantly higher in mouse Models I and II
than in normal mice. Daily oral administration of
a decoction of two Yin tonics-Radix Rehmanniae
(RR) and Plastrum Testudinis (PT) reduced peak
cAMP in both models. 3H.DHA binding assay re
vealed significant elevations of the number of 3
adrenergic receptors in rat Models I and II. Decoc-
tion RR and PT restored the peaks to normal range.
Yang tonics Radix Aconiti praeparata (RAP) and
Cortex Cinnamomi (CC) showcd contradictory
effects on these models. It seems unlikely that the
action of the two tonics promotes the dlearance of
injected hormones, since injected hormone RIA iii
plasma showed no differences between Yin tonics
treated and untreated models. The therapeutic
effects of these two Yin tonics on "Yin deficiency"
syndrome of hyperthyroidism or hyperfunction of
the adrenal cortex is very probably related to their
actions on the O-adrenoreceptor-cAMP system as
stated. 相似文献
In order to evaluate the significance of the
sinus node function test by transvenous atrial
pacing in the diagno,sis of sick sinus syndrome
(SSS), sinus node recovery time (SRT), co]rrected
sinus node recovery time (CSRT) and total atrio-
sinus and sino-atrial conduction time (SACT)
are observed in 69 patients including non-SSS
group 39 cases and SSS group 30 cases.
According to our data, we suggest criteria
for sinus node dysfunction as follows. SRT>1,400
ms and/or A-V junctional esca.pe before sinus
recovery and;or secondary pause, CSRT>560 ms.
total SACT>300 ms. With l item abnormal, the
false positive rat.e in the non-SSS group is 7.7c7。
and the false negative rate in the S.SS group 3.30i"
with 2 items abnormal, the false positive rate in
the non-SSS group Oi and the false negative
rate in the SSS grOiup 6.7'70.
Atropine test results are compared with that
of atrial pacing, 95.8To and 84.2% are compatible
with each other in these 2 groups. In order to
cut the use of invasive technic to the minimum,
we propose performing the atropine test first.
Only in those with unexplainable test results
should the trial pacing test. be resorted to.. 相似文献