后牙复合树脂磨耗的临床评价

为探索评价复合树脂磨耗的最佳方法，本研究采用直接临床评价法和间接模型比较法，对以４种复合树脂修复的９３例磨牙面洞修复体进行了３年的评估。结果表明，直接法对修复体早期磨耗的评估不敏感，但对修复体其它方面的评价目前仍为首选；间接法评估修复体的磨耗更准确、敏感，量化的磨耗值便于统计学分析；直接法与间接法相结合可更客观，更全面地评价复合树脂修复体的临床效果。     

成人牙周炎治疗前后龈沟液蛋白质的电泳分析

目的通过分析成人牙周炎患者同一位点基础治疗前后１个月龈沟液（ｇｉｎｇｉｖａｌｃｒｅｖｉｃｕｌａｒｆｌｕｉｄ，ＧＣＦ）中蛋白成分的改变，以期发现能够反映牙周健康及疾病状况的特异蛋白。方法采用滤纸条法收集１２例成人牙周炎患者５６个位点ＧＣＦ样本，以十二烷基磺酸钠聚丙烯酰胺凝胶电泳（ＳＤＳＰＡＧＥ）法分离，ＩｍａｇｅＭａｓｔｅｒＴＭ电泳图象分析仪分析其中的蛋白质。结果治疗后蛋白区带数及非血清源低分子量１２０００，１００００蛋白的丰度及百分含量较治疗前显著减少（分别为Ｐ＜０００１和Ｐ＜００５），而３８０００蛋白百分含量呈增加趋势且与治疗前牙周探诊深度和ＧＣＦ量呈负相关（Ｐ＜００５）。结论分子量为１２０００，１００００的蛋白与牙周炎症有关；分子量３８０００的蛋白与疾病的好转有关     

激素替代疗法治疗女性灼口综合征的临床观察

目的 :观察尼尔雌醇治疗女性灼口综合征的临床效果。方法 :采用尼尔雌醇辅以孕激素的雌激素替代疗法治疗已停经的女性灼口综合征患者。结果 :激素治疗组与维生素治疗组相比有显著性差异 (P <0 .0 1)。结论 :舌感觉异常多发生在妇女更年期 ,内分泌紊乱起着重要作用 ,激素替代疗法可以明显缓解症状     

Fluoride and magnesium concentrations in human dental calculus obtained from Japanese and Chinese patients

Calculus deposited on a total of 68 permanent teeth from patients 30-60 years old from Nagoya in Japan and Beijing in China was investigated. An abrasive microsampling method was used to examine the fluoride (F) and magnesium (Mg) distribution, using a fluoride ion-specific electrode and atomic absorption spectrophotometry, respectively. F concentrations decreased from the surface towards the interior of the calculus. Mg concentrations, however, gradually rose towards the innermost surface adjacent to the tooth. In all parts of the depth profiles, the average concentrations of both magnesium and fluoride were higher in the Japanese than in the Chinese calculus. Towards the inner surface of the calculus, F and Mg concentrations were also much higher in the Japanese than in the Chinese group. A greater intake of sea foods and greater use of fluoride dentifrices are possible reasons for the higher F and Mg concentrations in the Japanese individuals.     

E&Qplus根管充填技术与侧压充填技术封闭能力的比较

目的：评价并比较E＆Qplus技术和侧压技术的根尖封闭能力。方法：选取64个新鲜拔除的单直根管下颌前磨牙。4个做为阴性和阳性对照，60个随机分为6组（每组10个），预备至同一标准，分别采用两种充填技术配合三种不同种类的封闭剂（ADSEAL；cortisomol；Apexit）充填。X线牙片评价两种技术根管充填影像质量，通过20g／L亚甲蓝染料渗透试验比较各组的根尖微渗漏。结果：①两种充填技术的根尖微渗漏有统计学差异（P〈0．05）。②E＆Qplus技术充填组3种封闭剂的根尖封闭能力无统计学差异（P〉0。05）。③侧压技术组3种封闭剂的根尖封闭能力有显著的差异，ADSEAL封闭剂最好，Apexit封闭剂居中，cortisomol封闭剂最差。④X线影像质量，E＆Qplus技术优于传统侧压技术（P〈0．05）。结论：E＆Qplus技术的根尖封闭能力优于传统侧压技术，不同种类封闭剂对两种充填技术的根尖封闭能力均有影响，但程度不同。     

Association of vitamin D receptor gene polymorphisms in Chinese patients with generalized aggressive periodontitis

Background and Objective:  The clinical features suggest that genetic factors may have a strong influence on susceptibility to aggressive periodontitis. The aim of this study was to investigate the association of vitamin D receptor gene polymorphisms with generalized aggressive periodontitis in Chinese patients.
Material and Methods:  A restriction fragment length polymorphism (RFLP) for 10,438,141 C to T (rs1544410, Bsm I), 10,382,063 A to G (rs731236, Taq I), 10,382,143 C to A (rs7975232, Apa I) and 10,416,201 A to G (rs2228570, Fok I) of vitamin D receptor gene was analysed by polymerase chain reaction, followed by digestion with restriction enzymes and gel electrophoresis. The genotypes of 51 generalized aggressive periodontitis patients and 53 periodontally healthy control subjects were analysed. The genotypic and allelic frequencies of each polymorphism site for the patients and control subjects were compared.
Results:  The distribution of vitamin D receptor Fok I genotypes and alleles between the two groups was significantly different ( p =  0.043 and p  = 0.012, respectively). The F allele seemed to increase the susceptibility of aggressive periodontitis (odds ratio = 2.02, 95% confidence interval = 1.16–3.50) in Chinese patients. There was no significant difference in the genotype distribution or the allele frequencies of vitamin D receptor Bsm I, Apa I and Taq I between two groups.
Conclusion:  The study indicates that Fok I polymorphism of vitamin D receptor gene might be associated with generalized aggressive periodontitis in Chinese patients. In addition, the carriage of F allele increases the risk of developing generalized aggressive periodontitis.     

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目的验证不同方法诊断下颌第二磨牙C形根管系统发生率的准确性。方法收集济南市口腔医院和山东大学附属省立医院自2007年3月至2007年11月间因各种原因拔除的下颌第二磨牙共90颗,分离出具有C形根管特征的共32颗,拍摄术前X线片后开髓和清理髓室,手术显微镜下观察并记录根管口的形态,将初尖锉插入根管内拍初尖锉片。分析术前X线片和手术显微镜对C形根管系统诊断的成功率。对C形根管系统的初尖锉片进行分析,评价初尖锉片对于判断C形根管系统解剖形态的意义。结果32颗具有C形根管特征的离体牙中,通过术前X线片可以诊断的为20颗;通过手术显微镜下根管口的形态可以诊断的为28颗。C形根管的初尖锉片上,19颗表现为Ⅰ型,9颗表现为Ⅱ型,4颗表现为Ⅲ型。结论C形根管系统的诊断应采取术前X线片与根管显微镜相结合的方法,初尖锉片对于判断C形根管系统的分型具有较大的意义。     

Electroacupuncture Attenuates Surgical Stress-Induced Reduction of T Lymphocytes through Modulation of Peripheral Opioid System

Objective:To investigate the action mechanisms of electroacupuncture(EA)on postoperative immunosuppression.Methods:Male C57 BL/6 mice(5–7 weeks old)were randomly divided into:the sham injury group,the surgical trauma stressed group,the EA group[surgery+2/100 Hz EA at Neiguan(PC 6)],and the EA+Nal(surgery+EA+intraperitoneal injection of naloxone).Abdominal surgical trauma stress mice model was established.EA was performed on bilateral PC 6 acupoints by an EA apparatus(2/100 Hz)for 20 min once a day for 3 days.The m RNA expressions of MOR,DOR,and KOR in thymus and L3-5 dorsal root ganglions(DRG)were determined by quantitative real-time polymerase chain reaction(q RT-PCR)and the protein expressions of MOR,DOR,and KOR in thymus were measured by Western blot.Flow cytometry assay was used to detect the levels of T lymphocyte subtypes in the peripheral blood.Results:Surgical trauma induced decreased the m RNA expression level of MOR in both thymus(P<0.01)and L3-L5 DRGs(P<0.05).Moreover,EA treatment not only significantly attenuated the MOR protein and m RNA expression in the thymus(both P<0.05),but also markedly increased expression of DOR and KOR opioid receptor in thymus(P<0.01).However,the m RNA expressions of opioid receptors were not regulated by EA in the DRG(all P>0.05).Furthermore,T lymphocyte population of CD3^+ and CD4^+ was decreased in the peripheral blood after surgical trauma(both P<0.01).EA treatment can significantly elevate the population of CD3^+(P<0.01),CD4^+(P<0.05)and CD8^+T cells(P<0.01).Intraperitoneal injection of the non-selective opioid receptor antagonist naloxone blocked the up-regulation of T lymphocytes by EA.Conclusion:EA may improve postoperative immunosuppression through the peripheral opioid system.