全文获取类型
收费全文 | 22927篇 |
免费 | 1537篇 |
国内免费 | 228篇 |
专业分类
耳鼻咽喉 | 410篇 |
儿科学 | 346篇 |
妇产科学 | 390篇 |
基础医学 | 3837篇 |
口腔科学 | 360篇 |
临床医学 | 1927篇 |
内科学 | 4180篇 |
皮肤病学 | 1118篇 |
神经病学 | 1389篇 |
特种医学 | 1438篇 |
外科学 | 3001篇 |
综合类 | 90篇 |
一般理论 | 2篇 |
预防医学 | 1097篇 |
眼科学 | 522篇 |
药学 | 2368篇 |
中国医学 | 358篇 |
肿瘤学 | 1859篇 |
出版年
2023年 | 149篇 |
2022年 | 553篇 |
2021年 | 763篇 |
2020年 | 418篇 |
2019年 | 613篇 |
2018年 | 680篇 |
2017年 | 557篇 |
2016年 | 869篇 |
2015年 | 1199篇 |
2014年 | 1416篇 |
2013年 | 1542篇 |
2012年 | 2257篇 |
2011年 | 2059篇 |
2010年 | 1391篇 |
2009年 | 1067篇 |
2008年 | 1410篇 |
2007年 | 1302篇 |
2006年 | 1133篇 |
2005年 | 1076篇 |
2004年 | 832篇 |
2003年 | 688篇 |
2002年 | 606篇 |
2001年 | 286篇 |
2000年 | 283篇 |
1999年 | 216篇 |
1998年 | 103篇 |
1997年 | 82篇 |
1996年 | 72篇 |
1995年 | 50篇 |
1994年 | 47篇 |
1993年 | 44篇 |
1992年 | 83篇 |
1991年 | 72篇 |
1990年 | 63篇 |
1989年 | 59篇 |
1988年 | 62篇 |
1987年 | 47篇 |
1986年 | 56篇 |
1985年 | 59篇 |
1984年 | 44篇 |
1983年 | 54篇 |
1981年 | 20篇 |
1980年 | 17篇 |
1979年 | 33篇 |
1978年 | 30篇 |
1977年 | 21篇 |
1976年 | 19篇 |
1974年 | 18篇 |
1973年 | 25篇 |
1971年 | 16篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
81.
Recessive dystrophic epidermolysis bullosa (RDEB) is an uncommon and severe disorder characterised by trauma-induced blisters, intractable skin ulcers, scarring, milia, and nail dystrophy. We report the good result of both surgical release of fingers allowing spontaneous epithelisation without skin grafting and post-operative meticulous skin care without splinting, followed-up for one year. 相似文献
82.
We performed transurethral resections of the prostate in 100 patients under local anesthesia supplemented when appropriate by intravenous medication. In the first part of the study (40 patients) local anesthesia was infiltrated into the prostate transurethrally only, while in the second part (60 patients) infiltration was performed transurethrally and perineally. Prostate sizes ranged from 5 to 50 gm., and pain control was excellent in the majority of the patients, especially when perineal infiltration was added to the procedure. Of the patients 98 per cent said they would do it again, no patient required conversion to general anesthesia and there were no significant complications relating to the anesthesia. Local anesthesia with intravenous supplementation can be performed successfully in the majority of the patients with small to moderately sized prostate glands. 相似文献
83.
Yang Hee Lee Ihn Rhan Lee Won Sick Won Chung Hee Park 《Archives of pharmacal research》1988,11(3):247-249
84.
Proposed definition of a smokeless tobacco user based on “potential” nicotine consumption 总被引:1,自引:0,他引:1
Kathleen L. Schroeder Moon S. Chen Jr. Gregory R. Iaderosa Elbert D. Glover Elizabeth W. Edmundson 《Addictive behaviors》1988,13(4):395-400
This study examined and operationally defined "light," "moderate," and "heavy" users of smokeless tobacco according to the potential amounts of nicotine consumed per week from chewing tobacco and/or snuff products. Fifty adult male smokeless tobacco users ranging in ages from 18-85 were tested to determine nicotine content of smokeless tobacco products consumed. The smokeless tobacco products were blindly analyzed by gas chromatography to determine nicotine content. Mean nicotine yield per week was calculated by multiplying the number of cans and/or pouches used per week and placed on a normal probability distribution. The mean nicotine yielded 238 mg/wk and the categories emerged as "light" less than or equal to 87 mg/wk, "moderate" 88-388 mg/wk and "heavy" users greater than or equal to 389 mg/wk. Differences regarding type of user (light, moderate, heavy) were crossed tabbed with educational levels, years of smokeless tobacco use, age the habit was initiated, perception of harm and whether habit forming, and reason for using smokeless tobacco. 相似文献
85.
Growth factor-dependent activation of the MAPK pathway in human pancreatic cancer: MEK/ERK and p38 MAP kinase interaction in uPA synthesis 总被引:3,自引:0,他引:3
Increased expression of the hepatocyte growth factor (HGF) receptor (c-met) and urokinase type plasminogen (uPA) correlated
with the development and metastasis of cancers. To investigate the role of HGF/c-met signaling on metastasis in cancer cells
stimulated with HGF, we examined the effects of a specific MEK1 inhibitor (PD98059) and a p38 MAP kinase inhibitor (SB203580)
on HGF-induced uPA expression in pancreatic cancer cell lines, L3.6PL and IMIM-PC2. Pretreatment of PD98059 decreased HGF-mediated
phosphorylation of extracellular receptor kinase (ERK), uPA secretion and expression of matrix metalloproteinases (MMP-2 and
MMP-9) in a dose-dependent manner. In contrast, SB203580 pretreatment increased HGF-stimulated ERK phosphorylation, uPA secretion
and expression of MMPs. SB203580 also reversed the inhibition of HGF-mediated ERK activation and uPA secretion in the PD98059-pretreated
cells. These results suggest that ERK activation by HGF might play important roles in the metastasis of pancreatic cancer
and the p38 MAPK pathway also involved in the HGF-mediated uPA secretion and metastasis by regulation of ERK pathway.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
86.
A randomized comparison of pre-and post-experimental design was used to examine the effects of a fall prevention exercise program (FPEP) on muscle strength, ankle flexibility, balance, instrumental activities of daily living (IADLs), and depression for the community-dwelling elderly in Korea. Twenty-two subjects were assigned to an experimental group and twenty-three to a control group. The experimental group participated in a 4-day-per week FPEP of 8 weeks duration, twice a week by direct instruction and twice a week with videotaped instruction by the program instructor at each senior center. The eight-week FPEP turned out to be significantly effective in enhancing muscle strength, ankle flexibility and balance, and in reducing depression, after intervention among community-dwelling elderly in Korea. IADLs, however, was not changed by the intervention. Further research with a larger sample and longer follow up period is needed to expand our understanding about the effects of FPEP. Future study is also recommended to differentiate between the effects of FPEP by direct instruction and by videotaped. 相似文献
87.
The application of molecular techniques to solid tumors 总被引:1,自引:0,他引:1
Recent developments in the field of molecular biology including the sequencing of the human genome and related high throughput methodologies are presenting the diagnostic pathologist with new opportunities to expand our understanding of human disease. These techniques enable the comprehensive assessment of molecular alterations with cell populations of interest, including cancer. It will be necessary for the diagnostic pathologist to become familiar with these techniques to effectively translate their potential into the clinical environment. 相似文献
88.
Identification of Potential Diagnostic and Vaccine Candidates of Helicobacter pylori by Two-Dimensional Gel Electrophoresis, Sequence Analysis, and Serum Profiling 总被引:3,自引:0,他引:3
下载免费PDF全文
![点击此处可从《Clinical and Vaccine Immunology : CVI》网站下载免费的PDF全文](/ch/ext_images/free.gif)
C. Patrick McAtee Moon Young Lim Kevin Fung Mark Velligan Kirk Fry Theresa Chow Douglas E. Berg 《Clinical and Vaccine Immunology : CVI》1998,5(4):537-542
There is great interest in characterizing the proteins of the gastric pathogen Helicobacter pylori, especially those to which humans respond immunologically, because of the potential importance of such proteins in diagnosis and vaccine development. Two-dimensional gel electrophoresis was used to separate and identify potential antigens of H. pylori ATCC 43504. Over 30 proteins were reactive in Western blots with pooled sera from 14 infected patients. These proteins were analyzed by N-terminal sequence analysis. Fourteen proteins were determined to be distinct from any proteins previously described from H. pylori; the others were previously isolated and characterized proteins. Analysis of eight distinct H. pylori strains showed that most of these antigens were produced by all of the strains. We propose that collection of new antigens such as those recognized here will be useful in serologic tests for detecting and monitoring H. pylori infection and may also serve as potential targets for antimicrobial agent or vaccine development. 相似文献
89.
Deborah U Frank Sarah A Elliott Eon Joo Park Jennetta Hammond Yukio Saijoh Anne M Moon 《Developmental dynamics》2007,236(4):1085-1092
We targeted the reverse tetracycline controlled transactivator (rtTA) to the Foxa2 locus (Foxa2(ITA)) to generate a system for regulating Cre-recombinase activity within Foxa2 expression domains, including the endoderm, notochord, and floor plate of early mouse embryos. The use of an internal ribosomal entry site to obtain rtTA expression preserves Foxa2 function of the targeted allele. Cre activity with this system reflects the level of endogenous Foxa2 activity and is also tightly controlled by doxycycline. The location of Cre activity within the broader Foxa2 expression domain can be restricted by altering the timing of doxycycline administration. Isolated floor plate expression can be obtained in this manner. This system will provide a useful tool for manipulating gene expression in endoderm, notochord, and floor plate, all of which are tissues with important structural and patterning functions during embryogenesis. 相似文献
90.
Detection of YMDD motif mutants by oligonucleotide chips in lamivudine-untreated patients with chronic hepatitis B virus infection 总被引:9,自引:0,他引:9
Heo J Cho M Kim HH Shin YM Jang HJ Park HK Kim CM Kim GH Kang DH Song GA Yang US 《Journal of Korean medical science》2004,19(4):541-546
Lamivudine, a nucleoside analogue, has been used widely as an effective antiviral agent for the treatment of patients with chronic hepatitis B virus (HBV) infection. However, the YMDD motif mutation of HBV polymerase resistant to lamivudine occurs very frequently after long term therapy. We developed an oligonucleotide chip for the detection of YMDD motif mutants resistant to lamivudine and investigated the prevalence of the mutants in patients with chronic HBV infection who had not been treated by lamivudine before. Forty patients who had not been treated with lamivudine were included in this study. Serum samples were tested by the oligonucleotide chips designed for detection of wild-type YMDD motif, M552V and M552I. Samples were confirmed by restriction fragment length polymorphism (RFLP) and direct sequencing. M552I mutants were detected by the oligonucleotide chips in 7.5% (3/40) of chronic HBV infected patients (2 chronic hepatitis and 1 cirrhosis). The results were in accordance with those of RFLP. YMDD motif mutants occur as natural genome variabilities in patients with chronic HBV infection who had not been treated with lamivudine before. Oligonucleotide chip technology is a reliable and useful diagnostic tool for the detection of mutants resistant to antiviral therapy in chronic HBV infection. 相似文献