高效液相色谱法测定四川粉葛中葛根素的含量

目的：采用HPLC法测定四川粉葛中葛根素的含量。方法：色谱柱为依利特C18柱（4．6min&;#215;150mm,5μm）,流动相为甲醇-水（25：75）,流速为0．8ml／min,检测波长为250nm,柱温为25℃。结果：葛根素在0．0880～0．7040μg范围内峰面积与进样量呈良好的线性关系,回归方程为A=336．35X-1．7716,r=1．0000,其平均同收率为100．01％,RSD为0．14％（n=6）。结论：该方法简便易行,结果准确,可用于四川粉葛药材的质量控制。     

术前血糖水平对糖尿病患者行输尿管软镜碎石术后发生感染相关并发症的影响

观察2型糖尿病（T2DM）患者行输尿管软镜碎石术（FURL）后出现感染相关并发症的影响因素,探讨T2DM患者术前血糖水平与发生术后感染相关并发症的关系,为T2DM患者在接受FULR术后发生感染相关并发症的早期预防及治疗提供参考。     

Refractory Angina

The regimen for treating refractory angina is similar to that for impending infarction. Specific approaches to treatment include drugs and other medical methods. Operative intervention is reserved for those patients who do not respond to medical measures.     

Pediatric Power Wheelchairs: Evaluation of Function in the Home and School Environments

The purpose of this study was to compare the performance of four power wheelchairs when used by children to perform a variety of indoor activities. Eight boys and eight girls performed a series of 11 functional tasks when positioned in each of four different power wheelchairs reflecting different design classes. There were no significant differences for many of the dependent variables. However, the Everaid Turbo was significantly better for functional positioning at a standard kitchen table and at school desks and for accessing objects at different heights. By contrast the Invacare Jaguar was better for straight-line driving speed. Other findings include children's subjective impressions of the four power wheelchairs.     

Oncologic uses of the retrograde femoral nail.

Destructive lesions about the distal femur represent difficult treatment situations. Patients who present with impending or pathologic fractures require a method of fixation that provides adequate stabilization to allow for early, pain-free ambulation. Other considerations include restoration of functional range of motion, low perioperative morbidity and mortality risk, and minimal soft tissue dissection. Eleven patients with 12 pathologic or impending fractures of the distal femur who were treated with retrograde intramedullary nailing with and without polymethylmethacrylate augmentation were retrospectively reviewed. Followup averaged 17 months. Postoperative range of motion averaged 94 degrees flexion, 90% of patients reported no or modest pain, 81% were functioning with no restrictions or recreational restrictions only, and all patients were enthusiastic or satisfied with their surgical outcome. There were no perioperative mortalities, but one patient had 2 cm shortening secondary to broken distal locking screws and asymptomatic nail protrusion into the knee. Using the system for functional evaluation of reconstructive treatment of tumors of the musculoskeletal system, the average score for each patient was 25 (range, 18-30) or 83% (60%-100%) of the maximum score. The retrograde nail was an easy and successful method of fixation in this clinical setting.     

寰椎椎弓根螺钉置钉技巧探讨

[目的]探讨寰椎椎弓根螺钉固定治疗寰枢椎不稳的置钉技巧。[方法]总结2000年10月～2008年9月应用寰椎椎弓根螺钉技术治疗寰枢椎不稳患者66例,置钉132枚。术中在直视下依据手感行寰椎椎弓根螺钉置钉,复位固定。[结果]本组66例132枚螺钉均成功置入,复位固定满意。术中未发现脊髓、神经根、椎动脉等损伤。术后患者症状及神经脊髓功能均有不同程度恢复。96枚螺钉术后行CT检查,其中91枚位置良好(94.89%)。全部病例内固定无松动、断裂,植骨均融合。[结论]术前影像学测量,术中显露寰椎后弓、直视下依据手感置钉、X线确定进钉方向,是安全置钉的技巧。     

The predictive power of preoperative hip range of motion for the development of heterotopic ossification

Postoperative development of heterotopic ossification can compromise the success of total hip arthroplasty (THA). Heterotopic ossification has been associated with decreased postoperative hip range of motion (ROM), potentially leading to poor patient satisfaction with outcome. Many risk factors predisposing to heterotopic ossification have been discussed in the literature, including sex, age, operative time, surgical approach, and preoperative function. The goal of this study was to examine if preoperative ROM is a risk factor for the development of severe heterotopic ossification after THA, and the impact of severe heterotopic ossification formation on the gain in ROM following THA. In a retrospective study of a single surgeon's 20-year experience, all patients who developed type III heterotopic ossification after THA were evaluated for hip ROM preoperatively and at 1-year follow-up. Total ROM was classified according to the modified Merle d'Aubigne score, and Harris Hip Scores were calculated. A statistically significant difference was found in preoperative external rotation in the study group compared to the control group (P<.001). At 1 year postoperatively, hip ROM differences were significant in external rotation (P<.001), internal rotation (P<.001), and abduction (P<.05). The modified Merle d'Aubigne score was significantly different between the groups (P<.001). Although many factors have been shown to influence the development of heterotopic ossification following THA, we found that a decrease in preoperative external rotation may point to an increased risk. Surgeons should consider this data when considering the use of prophylactic treatment to avoid the development of heterotopic ossification.     

上调肝脏HO-1抑制肥大细胞脱颗粒减轻大鼠肝脏缺血再灌注损伤

目的 探索HO-1对肝脏缺血再灌注损伤中肥大细胞脱颗粒的影响。方法 将20只SD大鼠随机分成4组：假手术组（Sham组）,缺血再灌注损伤组（I/RI组）,HO-1诱导剂钴原卟啉组(CoPP组,术前24h给予CoPP,5 mg/kg)及HO-1抑制剂锌原卟啉组(ZnPP组,术前24h给予ZnPP,20 mg/kg)。建立大鼠缺血再灌注损伤模型,各组于再灌注后2h收集标本。RT-PCR检测肝脏组织HO-1 mRNA表达,Western blot检测肝脏组织HO-1蛋白表达;测定血清中ALT、AST水平;肝脏组织甲苯胺蓝染色检测肥大细胞脱颗粒数量,HE染色评价肝脏组织损伤情况。结果 与Sham组相比,I/RI组、CoPP组、ZnPP组大鼠组织HO-1 RNA和蛋白表达增加,血清ALT、AST水平升高,肥大细胞脱颗粒数量增多,肝脏细胞损伤加重。CoPP组与I/RI组相比,HO-1 mRNA和蛋白表达增加,血清ALT、AST水平减低,肥大细胞脱颗粒数量减少,肝细胞损伤减轻。ZnPP组与I/RI组相比,HO-1 mRNA和蛋白表达减少,血清ALT、AST水平升高,肥大细胞脱颗粒数量增多、肝细胞损伤严重。组间比较差异具有统计学意义(P＜0.05)。结论 HO-1过表达能减轻肝脏I/RI,其机制可能与抑制肝脏组织中肥大细胞脱颗粒有关。