KTP Laser and Neutral Red Phototherapy of Human Squamous Cell Carcinoma

Neutral red (NR) is a cationic, nontoxic vital dye employed as a histologic stain for proliferating cells; it has been used clinically for photodynamic treatment of herpes simplex virus lesions. NR is selectively taken up and concentrated by mitotic cells, an important characteristic for more effective antineoplastic agents. In the present study, UCLA-SO-P3 human squamous carcinoma cells displayed minimal toxicity when incubated with up to 50 μg/ml NR in the absence of light. However, cells incubated with greater than 0.5μg/ml NR followed by exposure to KTP laser light at 532 nm exhibited nearly 100% tumor cell death. The degree of cell toxicity was proportional to NR dose and laser light fluence. This study demonstrates that NR is an excellent cancer cell photosensitizer in vitro, and, after adding additional in vivo preclinical testing, may prove to be a useful agent in photodynamic destruction of head and neck tumors.     

A simplified technique for embryo biopsy: Use of the same micropipette for zona drilling and blastomere aspiration

Purpose: Using different micropipettes for zona drilling and blastomere aspiration for embryo biopsy is prevalent at centers of preimplantation genetic diagnosis. The purpose of our study was to simplify the technique by using only one micropipette. Methods: In this animal model, ICR mouse embryos at the four-cell stage (n=446) were randomly allocated into two groups: a biopsied group (n=224) for blastomere aspiration and a control group (n=222) without micromanipulation. We used a drilling/biopsy micropipette to drill a hole in the zona by expulsion of acidified Tyrode’s solution and to aspirate the blastomere by gentle suction with the same micropipette and pull it out of the zona. One blastomere was biopsied from each embryo. Results: In all, 222 (99.1%) intact blastomeres were successfully biopsied from 224 embryos. Only two blastomeres were damaged during aspiration. The capacity for blastocyst development (92.4 vs 93.7%) was not different between the two groups, but the percentages of embryos hatching (51.8 vs 18.0%) and hatched (29.9 vs 8.1%) were significantly higher in the biopsied group than in the control group. Conclusions: This simplified technique of embryo biopsy is safe and highly efficient for obtaining blastomeres for preimplantation genetic diagnosis and may also facilitate hatching of the blastocysts.     

声门上型喉癌临床N_0病理转移的观察

目的：为探讨喉癌颈淋巴结转移的规律，选择手术方法。方法：采用１１０例临床Ｎ０声门上型喉癌的１６４侧颈廓清术标本淋巴结连续切片方法观察。结果：发现颈淋巴结转移率为３５．５％（３９／１００）。提出临床Ｎ０病理转移的特点：１）转移淋巴结大小大多数介于０．５～１．５之间占８４．２％。２）转移淋巴结大多数为早期侵入期和生长发展期占８６．０％。３）转移淋巴结绝大多数为单发型占７４．４％。４）各个Ｔ分期均有转移淋巴结。结论：在颈淋巴结处理上，我们支持尽可能同期行选择性颈廓清术的观点     

Focal cerebral ischemia alters the spatio-temporal properties, but not the amount of locomotor activity in mice

Cerebral ischemia induces hyperlocomotion in gerbils, and sensorimotor and cognition dysfunctions in rodents ; however little is known about the spatio-temporal organization of locomotor activity after ischemia. In this study, we continuously （22 h） assessed the spatio-temporal organization of locomotor activity in focal cerebral ischemic mice in an open field with feeding and drinking supplies. To observe the spatio-temporal changes,     

青春期的性生理特征及其卫生教育

了解青春期的生理变化特征及其卫生,对青少年身心的健康成长和良好心理素质的培养具有重要意义.文章通过对青春期性心理特征的分析,重点阐述了如何对青少年加强性心理与心理卫生的教育.     

颞下窝咽侧区肿瘤的诊断与手术治疗

目的 寻求颞下窝咽侧区肿瘤的早期诊断及有效的手术途径。方法1978～2002年我院手术治疗颞下窝咽侧区肿瘤75例。手术采用4种进路：①颌下进路。②截断下颌骨颌下联合进路。③耳前颌下进路。④经上颌窦前外进路。绝大多数采用第1种手术入路。恶性肿瘤术后行放疗或化疗；继发性肿瘤在切除原发性肿瘤的同时予以切除。结果手术效果良好，无并发症。良性肿瘤38例术后6个月～18年(其中26例超过5年以上)，1例复发。恶性肿瘤37例中，12例死亡，5例失访，余20例观察3～5年无复发及转移。结论①颞下窝咽侧区肿瘤CT扫描及针吸细胞病理检查是有效的诊断手段。②颌下进路是该区肿瘤较好的手术途径。③术中彻底止血、尽可能消灭死腔及负压引流对预防切口感染和预防呼吸道阻塞都相当重要。     

激光治疗阻塞性睡眠呼吸暂停低通气综合征疗效评价

目的：探讨Nd：YAG激光治疗阻塞性睡眠呼吸暂停低通气综合征(OSAHS)的疗效。方法：对89例OSAHS患者行激光悬雍垂腭咽成形术(LAUP)．手术切除部分软腭及悬雍垂，在悬雍垂两侧向上楔形打开软腭。结果：术后6个月复查有76例行多导睡眠监测，治愈8例，显效36例，有效25例，无效7例，总有效率90．8％。结论：Nd：YAG激光具有止血凝固作用且穿透力强，手术视野清晰，并发症少，可缩短手术时间。LAUP治疗OSAHS效果良好。     

Effects of storage and reperfusion oxygen content on substrate metabolism in the isolated rat lung

BACKGROUND: Lung transplantation requires a period of storage and ischemia; we examined the largely unknown effects of that period on intermediary metabolism. METHODS: Two groups of isolated rat lung blocks (n = 16 each) were flushed with Euro-Collins solution and harvested. The lung blocks were immediately ventilated and either perfused for 30 minutes with an erythrocyte-based solution containing carbon 13 labeled substrates (group 1) or stored for 6 hours at 1 degree C and then reperfused (group 2). Half of each group was reperfused at a physiologic Po2 the other half at high Po2. Analysis of carbon 13 isotopomers was performed to determine substrate utilization through aerobic pathways in lung tissue. RESULTS: Lungs from both groups oxidized all major substrates. The contribution of fatty acids to acetylcoenzyme acid oxidized in the citric acid cycle was significantly higher in group 2 than in group 1 (31.3% +/- 2.2% versus 22.0% +/- 2.1%, p < 0.05). Perfusate Po2 did not affect substrate preference. Gas exchange was worse in stored lungs. CONCLUSIONS: After a period of hypothermic ischemia and storage, substrate preference in lung tissue exhibits a switch towards fatty acids. As fatty acid oxidation occurring after ischemia is deleterious in other organs, strategies to inhibit this process in stored lungs may warrant further investigation.     

MVD及VEGF在颅咽管瘤中的表达及其与预后的关系

背景与目的：颅叫管瘤为难治性肿瘤，其预后不良，复发机制尚不清楚。本研究检测颅Ⅱ阏管瘤组织中微血管密度（microvascular density，MVD）及血管内皮生长因子vascular endothelial growth factor，VEGF表达．分析其与肿瘤预后等生物学行为的关系。方法：采取前瞻性队列研究方案，经纳入、排除标准筛选卅颅咽管釉质上皮型肿瘤32例、鳞形乳头瘤型肿瘤31例，平均随访843个月，收集原发及复发患者的肿瘤组织标本，应用单克隆抗体CD34、VEGF抗体检测肿瘤组织MVD及VEGF蛋白表达，计算机辅助成像系统对其作出定量分析，分析其与肿瘤复发等生物学行为的关系。结果：VEGF蛋白、MVD计数在颅咽管瘤队列复发组、非复发组间不具有显著性差异（P〉0．05），而在颅咽管瘤不同病理类型间差异则具有显著性（P〈0．05）。结论：VEGF蛋白、MVD计数与肿瘤复发无关，不能预测颅咽管瘤复发的危险性，但可为了解肿瘤的同有侵袭性行为提供参考。     

COX-2 基因与基质金属蛋白酶在星形细胞瘤中的表达及二者相关性研

 目的 探讨COX-2基因与基质金属蛋白酶在星形细胞瘤中的表达及二者相关性。方法 用原位杂交的方法检测星形细胞瘤组织中COX-2 mRNA的表达；用Ultrasensitive S-P法(链霉素抗生物素蛋白-过氧化物免疫组化染色)检测同等标本中基质金属蛋白酶-2和基质金属蛋白酶-9蛋白的表达；并将COX-2 mRNA与基质金属蛋白酶的表达作相关性分析。结果 COX-2 mRNA及MMP2、MMP9在星形细胞瘤中高度表达，表达阳性率分别为62．69％、56．70％和58．20％；COX-2 mRNA与MMP2和MMP9均有正相线性关系，Pearson积距相关系数分别为0．260、0．347，P<0．05。结论 COX-2基因和基质金属蛋白酶在星形细胞瘤组织中的表达密切相关，说明它在星形细胞瘤向周围组织侵袭中起重要作用。