嗅沟脑膜瘤术式选择及长期临床预后随访

目的分析嗅沟脑膜瘤的手术治疗和随访结果。方法收集20例嗅沟脑膜瘤手术病例,并分析其临床表现及随访结果。结果嗅沟脑膜瘤大多以精神症状、视觉障碍和嗅觉减退为首发症状,9例行冠状切口,4例行半冠状切口,分块切除肿瘤,5例翼点入路,1例纵裂入路。1例眶上入路,SimpsonI级切除6例。SimpsonⅡ级切除13例,SimpsonⅢ级切除1例,预后良好,1例复发。随访时间1~6年(平均3.1年),术前术后均保留嗅觉的为2例,精神状态改善18(91%)和视力障碍改善的15(75%)。1例(5%)复发再次手术。结论术式的选择取决诸多因素,预后取决于手术中的组织保护。     

丝裂霉素C在翼状胬肉切除联合自体结膜瓣移植术中的应用

目的 讨论安全有效浓度下，丝裂霉素C在翼状胬肉切除联合体自体结膜移植术中的应用，观察对翼状胬肉的复发的防治效果。方法 对54例64眼原发性胬肉者施行显微镜下胬肉切除带蒂结膜瓣转位移植联合术中或术后丝裂霉素C（mitomycin C MMC)的应用，经5－12月随诊，与单纯手术组（27例33眼）原发性胬肉术后复发情况作对比分析。结果 复发率要术中用药组为3．33％，术后用药组5．88％，对照组18．18％，经X^2检验，术中、术后用药与对照组 差异有显著性（P＜0．05），而术中或术后应用MMC的复发率差异无性，均可降低胬肉的复发率。结论 适时、适量应用MMC对有效防止翼状胬肉的复发有着非常重要的意义。     

应用心肌分层应变及做功技术评估长期腹膜透析尿毒症患者左心室功能

［摘要］目的： 利用心肌分层应变及心肌做功技术对长期腹膜透析的尿毒症患者的左心室功能进行研究,评估其在长期腹膜透析下的心功能变化。方法: 选择长期规律进行腹膜透析且经常规超声心动图检查提示左心室射血分数(left ventricular ejection fraction,LVEF)正常的尿毒症患者14例,测量其常规二维超声心动图参数,利用二维斑点追踪及心肌分层应变技术测量左心室整体纵向收缩期峰值应变(global longitudinal peak systolic strain,GLS)及内膜层、肌层和心外膜层3层心肌的纵向收缩期峰值应变(longitudinal peak systolic strain,LS),并结合血压得出左心室心肌整体做功参数,共同评估左心室心肌功能,随访5年并比较腹膜透析5年前后相关数据变化。结果: 规律腹膜透析5年后,尿毒症患者LVEF改变无统计学差异;GLS在四腔心切面明显减低(P=0.018),并从心内膜减低至心外膜(LS心内膜层 P=0.029,LS肌层 P=0.017,LS心外膜层 P=0.004);心肌整体做功参数以整体无用功(GWW)升高为主(P=0.048),整体有用功(GCW)、整体做功指数(GWI)及整体做功效率(GWE)无统计学差异。结论: 长期规律腹膜透析5年的尿毒症患者,其左心室整体心肌功能尚保持稳定,但局部心肌收缩功能进一步受损,主要体现在后间隔和侧壁的各层心肌,心肌做功以GWW升高为主。     

宫颈粘液过氧化物酶在月经周期中的变化规律

本文对29例月经周期正常妇女的宫颈粘液过氧化物酶进行了30个周期的研究。在月经周期不同时间测定宫颈粘液过氧化物酶(CMPx)活性及血清促黄体生成素(LH)、雌二醇(E_2)和孕酮(P)。结果表明:在排卵前三天酶活性明显下降,至排卵后一天开始上升。卵泡期,酶活性与E_2呈负相关(r=-0.67);黄体期,酶活性与P呈正相关(r=0.79)。本研究提示:1.CMPx在排卵周期具有特定的变化规律,其变化受体内激素水平影响,可作为预告排卵的指标。2.如简化测定方法,可为自然避孕提供新途径。     

Scrub typhus vaccine candidate Kp r56 induces humoral and cellular immune responses in cynomolgus monkeys

A truncated recombinant 56-kDa outer membrane protein of the Karp strain of Orientia tsutsugamushi (Kp r56) was evaluated in cynomolgus monkeys (Macaca fascicularis) for immunogenicity and safety as a vaccine candidate for the prevention of scrub typhus. This recombinant antigen induced strong humoral and cellular immune responses in two monkeys and was found to be well tolerated. Antigen-specific immunoglobulin M (IgM) and IgG were produced to almost maximal levels within 1 week of a single immunization. Peripheral blood mononuclear cells from vaccinated animals showed an induction of antigen-specific proliferation and gamma interferon production. The Kp r56 was not as efficient as infection with live organisms in preventing reinfection but was able to reduce the inflammation produced at the site of challenge. This report describes the results of the first systematic study of the immunogenicity of a recombinant scrub typhus vaccine candidate in a nonhuman primate model.     

Peripheral Lymphocyte Subpopulations in Patients with Herpes Genitalis

Peripheral blood samples from 52 women including 16 with herpes genitalis and 36 healthy persons were studied to enumerate subpopulations of lymphocytes. T lymphocyte counts were done by SRBC rosette tests and B lymphocytes by immunobead rosette tests using antibody-coated polyacrylamide beads. It was found that the mean percentage of «active T lymphocytes was significantly less in the patients with herpes genitalis than in the controls (herpes genitalis; 13.9 ± 6.8%, controls; 25.0 ± 8.3%, p < 0.001). No difference was noted in the percentage of «total» T lymphocytes, «total» B lymphocytes and subsets of B lymphocytes (IgG-, IgA- and IgM-bearing lymphocytes) between the patients and controls. The present findings suggest that cell-mediated immune function associated with «active» T lymphocytes is suppressed in patients with herpes genitalis.     

Preparation and characterization of porous beta-tricalcium phosphate/collagen composites with an integrated structure

Porous beta-tricalcium phosphate (TCP)/collagen composites with different beta-TCP/collagen weight ratio were prepared. The influences of the preparation conditions on the microstructure of porous composite and the joint status of beta-TCP particles with collagen fibrils were characterized by X-ray diffractometer, scanning electron microscopy and transmission electron microscopy. The results showed: (1) an acid treatment could effectively disassemble collagen fibrils; (2) in the resulting porous composites, beta-TCP particles homogenously existed on the skeleton of the collagen fibril network and bonded tightly to both the fibrils and themselves. The tight bonding formation could be due to the reaction between Ca ions in the particles and carboxyl groups in collagen polypeptide chains and due to the reprecipitation of partially dissolved beta-TCP during synthesis. The tight bonding between beta-TCP particles and collagen fibrils in the composites demonstrated an integrated structure, which was reproducible when beta-TCP/collagen ratio ranged from 2 to 4. Such integrated structure would make significant contributions in reliably tailoring properties of the porous composites by varying beta-TCP content. In addition, the porous composites had large porosity (approximately 95%) and appropriate pore size (approximately 100 microm), showed no negative impact in cytotoxicity assay and complete bone tissue regeneration after 12 weeks in animal test.     

鸡卵黄抗体的制备以及金磁微粒为载体去除人血浆部分高丰度蛋白的研究

目的:以人血浆白蛋白(HSA)和IgG为免疫原,制备特异性鸡卵黄抗体IgY(Egg yolk immunoglobulin),并将其固定于金磁微粒表面,用于HSA和IgG的去除研究.方法:用HSA、IgG以及混合成分分别作免疫原免疫Roman母鸡.制备抗HSA和IgG鸡卵黄抗体IgY,并对IgY的分离提取条件进行优化.SDS-PAGE和间接ELISA检测IgY的纯度和效价.将高效价IgY固定于金磁颗粒表面进行血浆高丰度蛋白去除研究.结果:免疫后60～120 d内,鸡血清抗体效价可达1∶15 000～1∶25 000;收获鸡蛋,提取得到的卵黄抗体IgY效价可达1∶10000～1∶25000,纯度98%以上;采用金磁微粒载体固定IgY,可对血浆中的HSA,IgG进行特异性的去除.结论:人血浆中的高丰度蛋白成分HSA和IgG免疫产蛋母鸡后,可从鸡卵黄中分离提取到高效价、高纯度的卵黄抗体IgY;IgY偶联于金磁微粒表面可特异性的去除人血浆中的HSA和IgG,作为血浆蛋白质组学研究的一种新方法,有较好的应用前景.