Lancet子刊120万人研究:常出门运动的人,心理更健康

正33岁的阿甘,连续创业3次,最后在22层高楼上纵身一跳……一时间各种评论、哀悼、反思刷屏,所有的刷屏聚焦在一点:如何更好战胜心理困扰?近日,美国耶鲁大学学者发表在Lancet子刊一项对120万人进行的研究送来了"处方":只要走出房间去运动,就会让一个人心理更健康。该研究显示,一般人每个月总有那么三四天不开心,而那些积极锻炼的     

SARS-CoV-2 Variants,South Sudan,January–March 2021

As the coronavirus pandemic continues, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) sequence data are required to inform vaccine efforts. We provide SARS-CoV-2 sequence data from South Sudan and document the dominance of SARS-CoV-2 lineage B.1.525 (Eta variant) during the country''s second wave of infection.     

PROGNOSTIC VALUE OF MIB-1 IN NEUROENDOCRINE TUMOURS OF THE LUNG

The spectrum of neuroendocrine lung tumours ranges from highly aggressive small cell carcinomas (SCLC) to carcinoid tumours (CD) of low malignant potential. Between these two extremes, the ‘well-differentiated neuroendocrine carcinomas’ (WDNEC) form a transitional group with uncertain biological behaviour. This study investigated the prognostic value of the proliferation marker MIB-1 (paraffin Ki-67) in 59 neuroendocrine lung tumours (32 SCLC, 13 WDNEC, 14 CD) by immunostaining of routinely processed paraffin sections. Morphometric evaluation was done by semi-automatic image analysis. The results were compared with survival data (mean follow-up: 42 months). The proliferation rates of the tumours as determined by MIB-1 immunoreactivity (MIB-1-PR) were significantly different between the tumour types (SCLC>WDNEC>CD) and showed a strong inverse correlation with survival time. In CD, the percentage of MIB-1-labelled nuclei never exceeded 1·1 per cent; higher values would therefore favour the diagnosis of WDNEC over that of CD. Among WDNEC, MIB-1 was able to differentiate a subgroup with excellent prognosis (MIB-1-PR: 0·3–3·4 per cent) from another subgroup with a death rate of 50 per cent (MIB-1-PR: 7·3–20·3 per cent). Within each tumour type, all patients without distant metastases at diagnosis survived when MIB-1-PR was ⩽9·4 per cent, suggesting a potential threshold for prognosis. Although the status of metastases was the dominant prognostic factor in these neoplasms, MIB-1 was able to provide additional prognostic information allowing the definition of prognostically different subgroups of patients. In conclusion, MIB-1 and the status of metastases are complementary prognostic indicators and are best used in combination to characterize the biological behaviour of neuroendocrine lung tumours.     

针刺结合中药治疗抑郁症的临床优势探讨

目的：针刺结合中药治疗抑郁症效果良好,探讨其在临床应用中的优势。方法：采用汉密尔顿抑郁量表（HAMD）（24项）筛选轻、中度抑郁症患者,同时符合中医痰气郁结证,计54例,随机分为针刺加中药组（观察组）和单纯西药组（对照组）,各27例。采用汉密尔顿抑郁量表（HAMD）（24项）、中医证候积分评定临床疗效,副反应量表（TESS）评定不良反应。结果：52例完成试验,其中观察组25例,对照组27例。经8周治疗后,两组数据进行t检验后,经统计学分析,治疗组和对照组在治疗的第8周平均减分率〉50%,达到显著进步。治疗组和对照组在治疗前后HAMD减分率差异均有显著性意义（P〈0.05）,治疗组和对照组之间差异也有统计学意义（P≤0.05）,治疗组略优于对照组。治疗前后中医症候疗效比较,两组经χ2检验后,P〉0.05,说明治疗组与对照组临床疗效无显著性差异。两组治疗后进行TESS（副反应量表）的评定,两组比较差异有显著性意义（P〈0.05）,观察组的不良反应较对照组少而轻。结论：针刺治疗抑郁症疗效略优于单纯西药,但不良反应明显减少,而且症状较轻,更适合临床应用。     

亲缘异基因造血干细胞移植与自体造血干细胞移植治疗弥漫性大B细胞淋巴瘤大宗病例分析

选择自体造血干细胞移植还是异基凶造血干细胞移植治疗弥漫性大B细胞淋巴瘤（diffuse large B cell lymphoma,DLBCL）患者需权衡治疗相关死亡率（treatment—related mortality,TRM）差异、自体移植物被肿瘤污染的可能、干细胞动员失败和异基因移植物抗淋巴瘤效应等因素之间的利弊。     

铅对肾上腺皮质细胞线粒体的氧化损伤

目的】 研究铅对肾上腺皮质细胞氧化应激和线粒体功能的 影响,为了解其肾上腺皮质毒作用机制提供依据。【方法】 原代分离培养豚鼠肾上腺皮质 细胞,以0、6.25、12.5、25、50、100 μmol/L醋酸铅(PbAc)处理细胞,观察PbAc诱导肾 上腺皮质细胞活性氧(ROS)产生和线粒体损伤作用。ROS检测采用荧光分光光度法,线粒体膜 电位(MMP)和细胞存活状态采用Rh123和PI双标记、流式细胞术检测,细胞ATP水平采用化学 发光法测定。【结果】 PbAc染毒后肾上腺皮质细胞ROS形成水平随剂量增加而增加,具有剂 量效应关系[y=4.16+10.21×1g(x+1),P<0.01,R2=0.64 1］;线 粒体膜电位呈剂量依赖性降低,Rh123的平均荧光强度(MFI)在6.25～100 μmol/L各剂量组 依次为1.01,0.94,0.96,0.95和0.91,与对照组(1.35)比较具有显著性差异(P<0 .01);染毒后细胞死亡率轻度增加,与对照组(1.02%)比较,50 μmol/L和100 μmol/L的 PbAc组分别为3.16%和3.40%,差异具有显著性(P<0.05);ATP水平降低与PbAc剂量之 间存在剂量效应关系[y=212965.7-51592.5×1g(x+1),P<0.01,R2= 0.568］,剂量和时间对ATP水平的影响呈协同抑制作用(P<0.01)。【结论】 线粒体 氧化应激介导肾上腺皮质细胞毒性可能是PbAc毒作用机制之一,线粒体损伤是铅致肾上腺皮 质毒作用的早期细胞和分子事件。     

巴戟天多糖及其水提取物对体外培养成骨细胞活性的影响

目的:体外培养成骨细胞,观察巴戟天多糖和巴戟天水提物对成骨细胞活性的影响。方法:实验于2004-09/2005-08在福建省骨伤研究所骨伤分子生物学实验室完成。①提取24h内新生SD大鼠头盖骨成骨细胞,分别用50,100,200g/L巴戟天多糖及50,100,200g/L巴戟天水提物药物血清进行体外培养。对照组用含50,100,200g/L无药血清的DMEM培养液培养。培养72h后进行相关指标检测。②通过MTT方法检测巴戟天多糖、巴戟天水提取物对成骨细胞增殖的影响。③检测碱性磷酸酶、细胞内骨钙素、转化生长因子β1基因表达等指标,观察巴戟天多糖、巴戟天水提取物对成骨细胞活性的影响。结果:①药物血清对成骨细胞增殖的影响:100g/L巴戟天水提物组和100g/L巴戟天多糖组吸光度大于对照组,差异有非常显著性(P<0.01),且100g/L巴戟天多糖组吸光度高于100g/L巴戟天水提物组(0.4302±0.0193,0.3829±0.0228,P<0.01)。②药物血清对成骨细胞活性的影响:巴戟天水提物组与巴戟天多糖组碱性磷酸酶比活性、细胞内骨钙素含量、转化生长因子β1mRNA相对表达量均高于对照组[碱性磷酸酶比活性:(16.89±2.09),(20.68±2.44),(11.23±2.40)μkat/g;骨钙素含量:(3.74±0.82),(3.97±0.69),(2.63±0.34)ng/106;转化生长因子β1 mRNA相对表达量:2.97±0.37,4.23±0.30,0.07±0.27,P均<0.01]。巴戟天多糖碱性磷酸酶比活性、转化生长因子β1 mRNA相对表达量高于巴戟天多糖组(P<0.01)。结论:巴戟天水提物与巴戟天多糖均能显著促进体外培养成骨细胞增殖、促进成骨细胞分泌碱性磷酸酶与骨钙素、促进成骨细胞转化生长因子β1 mRNA的表达,并且巴戟天多糖优于巴戟天水提物。