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991.
目的:观察经椎旁肌肌间隙入路伤椎置钉短节段椎弓根螺钉内固定对单节段无神经损伤胸腰椎骨折的治疗效果。方法选取单节段胸腰椎骨折患者13例,采用椎旁肌间隙入路伤椎置钉短节段椎弓根螺钉内固定治疗,观察患者术后伤椎矢状面Cobb角、伤椎椎体前缘高度、疼痛、脊柱功能和并发症发生情况。结果本组患者手术均顺利完成,术后均获1年随访,切口甲级愈合;术后伤椎矢状面Cobb角均较术前明显缩小,手术前后比较差异有统计学意义( P﹤0.05);术后伤椎椎体前缘高度较术前明显增高( P﹤0.05);术后VAS评分及ODI均较术前明显下降( P﹤0.05)。治疗过程中均无感染、内固定松动或断裂等并发症发生。结论经椎旁肌肌间隙入路伤椎置钉短节段椎弓根螺钉内固定能有效纠正单节段胸腰椎骨折伤椎前缘高度,纠正后凸畸形,不良反应少。 相似文献
992.
目的 探讨高糖条件下感染携带肝细胞生长因子的重组腺病毒(Ad-HGF)对人脐静脉内皮细胞(humanumbilical vein endothelial cells,HUVECs)凋亡相关蛋白Bax、Bcl-2表达的影响.方法 将HUVECs分为低糖组(LG组,5.5 mmol/L)、高糖组(HG组,35 mmol/L)、腺病毒对照组(HG+ Ad-GFP组)和实验组(HG+ Ad-HGF组).检测4组HUVECs增殖情况、细胞内活性氧(ROS)水平及凋亡相关蛋白Bax、Bcl-2的表达.结果 HG组、HG+ Ad-GFP组HUVECs存活率低于LG组,HG+ Ad-HGF组高于HG组(P<0.05,P<0.01).HG组、HG+ Ad-HGF组HUVECs内ROS水平高于LG组,但HG+ Ad-HGF组低于HG组(P<0.05).HG组、HG+ Ad-GFP组Bax、Bax/Bcl-2高于LG组,Bcl-2低于LG组,但HG+ Ad-HGF组Bax、Bax/Bcl-2低于HG组,Bcl-2高于HG组(P<0.05).结论 感染Ad-HGF可通过降低细胞内ROS水平和Bax/Bcl-2减少细胞凋亡,进而对高糖诱导的HUVECs起到保护作用. 相似文献
993.
994.
历代医家对痛经的治疗,大多以理气行气药、活血化瘀药、补益药、温里药为主。其中汉唐时期以《金匮要略》《小品方》的通治方为主,相对的补益药为多;到了宋金元时期,大多以活血化瘀、温里、补益药为主,体现了此时期调气血的理论,治法基本是理气、活血、补气、养血、散寒五个方面;明代医家基本传承宋金元时期,以活血化瘀药和补益药的运用为主,增加清热药物的运用,并以经前痛多为实,经后痛多为虚为虚实辨证的原则;清代和民国医家,用药多重视补益,以著名医家傅青主为典范,认为治疗痛经应当重视调补肝肾法,用以补肾水、调肝气之药,反应了该时期用药的特色。 相似文献
995.
996.
化脓性颞下颌关节炎的特征与诊断 总被引:2,自引:0,他引:2
目的探讨化脓性颞下颌关节炎的临床特征及其早期诊断方法。方法收集我科近5年的30例化脓性颞下颌关节炎患者,对其临床症状,关节液的性质和量,关节液的组织学和细菌学表现,影像学检查,其它实验室检查及后遗症进行分析。结果患者主要为成年人(>18岁占29/30);血源性感染多见;多数(25/30)局部症状轻,全身反应不明显;关节液组织学检查见大量粒细胞,可伴纤维蛋白或纤维软骨碎片;15/30患者关节液细菌学检查见到细菌,培养出的病原菌主要为金黄色葡萄球菌和腐生葡萄球菌;后遗症轻,主要为继发性骨关节病。结论当今化脓性颞下颌关节炎表现出感染源隐匿性、症状不典型性、后遗症轻等特点;关节腔穿刺和关节液分析是其必要的诊断手段。 相似文献
997.
Objective: To study the burden of illness of low back problems—prevalence and consequences—in the working and the non-working population.Methods: Data from the Monitoring Project on Risk factors for Chronic Diseases, the MORGEN-study, were used. This project provided data on a probability sample of the general population aged 20–59 y in the Netherlands. Cross-sectional questionnaire data on 6317 men (24% non-working) and 7505 women (47% non-working) gathered over the period 1993–1995 were analysed.Results: The 12 month period prevalence of low back problems for the working and non-working population was 44.4% and 45.8% for men, and 48.2% and 55.0% for women. Larger differences were found for chronic low back problems, and activity limitation and use of health services due to low back problems. More than one-third of those who were disabled were so because of low back problems. When those unable to work because of disability (work disabled) were excluded, the prevalence and consequences of low back pain were still higher in the non-working group in comparison with the working population. Most of the non-working women were housewives and this group was both large in size and had a high prevalence of low back problems.Conclusions: Among the men studied, more than a quarter of the total burden of low back problems in those aged 20–59 y were found in the non-working population, among women this was 50%. Both research on causes and determinants of low back pain and the development of preventive actions—now being extensively focused on the working population—should also be translated to the non-working population. 相似文献
998.
多源信息融合在医学领域的应用研究 总被引:1,自引:0,他引:1
近年来,随着传感器性能的提高、信息处理技术的发展,面向各种复杂应用背景的多传感器系统大量涌现。在多传感器系统中,系统的信息来自于多个传感器,在信息处理的过程中多源信息表现形式的多样性,信息关系的复杂性,信息数量的巨大性,以及要求信息处理的及时性,都已大大超出了人脑的信息综合处理能力。为此,从20世纪70年代初期开始,多传感器信息融合(Multisensor Information Fusion)技术作为一门新兴的学科迅速发展起来,并在现代医学领域和各种其它民用领域得到广泛地应用。 相似文献
999.
Antinori S; Versaci C; Dani G; Antinori M; Pozza D; Selman HA 《Human reproduction (Oxford, England)》1997,12(2):286-291
Between July 1995 and May 1996, 36 patients with non-obstructive
azoospermia of secretory origin underwent intracytoplasmic injection of
spermatids. A previous histological biopsy was performed on all patients:
15 had spermatogenic arrest, a further 13 had Sertoli cell- only syndrome,
and the remaining eight had post-cryptorchidism tubal atrophy. The
ejaculate was duly examined and a complete absence of spermatozoa and
spermatids was confirmed, with only bacteria and debris being found.
Testicular sperm extraction (TESE) was then performed. In 19 out of 36
cases round spermatids only were found, while elongated spermatids were
found in the remaining 17. Both round and elongated spermatids were
isolated and used for injection. A total of 135 oocytes at metaphase II
were recovered from 19 partners and injected with round spermatids, while
123 mature oocytes from 17 partners were injected with elongated
spermatids. The number of oocytes fertilized, as judged by the presence of
two pronuclei, was 75 (55.5%) and 71 (57.7%) respectively. By 34 h after
injection, the number of embryos which had cleaved to the 2-cell stage was
56 (74.6%) with round spermatids and 55 (77.4%) with elongated spermatids.
All cleaved embryos were transferred into the uterus of the partners.
Clinical pregnancies were established in two cases of round spermatid
cycles (10.5%) (both are still ongoing), and three cases of elongated
spermatid cycles (17.6%) (two are still ongoing; one was lost after 8 weeks
of gestation). Chromosomal analysis showed that all fetuses had a normal
karyotype (three male and one female) with no chromosomal abnormalities.
相似文献
1000.
Bijvoet AG; Kroos MA; Pieper FR; Van der Vliet M; De Boer HA; Van der Ploeg AT; Verbeet MP; Reuser AJ 《Human molecular genetics》1998,7(11):1815-1824
Glycogen storage disease type II (GSDII) is caused by lysosomal acid
alpha-glucosidase deficiency. Patients have a rapidly fatal or slowly
progressive impairment of muscle function. Enzyme replacement therapy is
under investigation. For large-scale, cost-effective production of
recombinant human acid alpha-glucosidase in the milk of transgenic animals,
we have fused the human acid alpha-glucosidase gene to 6.3 kb of the bovine
alphaS1-casein gene promoter and have tested the performance of this
transgene in mice. The highest production level reached was 2 mg/ml. The
major fraction of the purified recombinant enzyme has a molecular mass of
110 kDa and resembles the natural acid alpha-glucosidase precursor from
human urine and the recombinant precursor secreted by CHO cells, with
respect to pH optimum, Km, Vmax, N-terminal amino acid sequence and
glycosylation pattern. The therapeutic potential of the recombinant enzyme
produced in milk is demonstrated in vitro and in vivo. The precursor is
taken up in a mannose 6-phosphate receptor-dependent manner by cultured
fibroblasts, is converted to mature enzyme of 76 kDa and depletes the
glycogen deposit in fibroblasts of patients. When injected intravenously,
the milk enzyme corrects the acid alpha-glucosidase deficiency in heart and
skeletal muscle of GSDII knockout mice.
相似文献