全文获取类型
收费全文 | 332篇 |
免费 | 19篇 |
国内免费 | 21篇 |
专业分类
耳鼻咽喉 | 6篇 |
儿科学 | 17篇 |
妇产科学 | 4篇 |
基础医学 | 23篇 |
口腔科学 | 4篇 |
临床医学 | 46篇 |
内科学 | 91篇 |
皮肤病学 | 10篇 |
神经病学 | 9篇 |
特种医学 | 78篇 |
外科学 | 8篇 |
综合类 | 8篇 |
预防医学 | 14篇 |
眼科学 | 2篇 |
药学 | 36篇 |
肿瘤学 | 16篇 |
出版年
2023年 | 2篇 |
2021年 | 5篇 |
2020年 | 3篇 |
2019年 | 1篇 |
2018年 | 3篇 |
2017年 | 3篇 |
2016年 | 4篇 |
2015年 | 7篇 |
2014年 | 9篇 |
2013年 | 13篇 |
2012年 | 7篇 |
2011年 | 15篇 |
2010年 | 8篇 |
2009年 | 11篇 |
2008年 | 11篇 |
2007年 | 25篇 |
2006年 | 4篇 |
2005年 | 10篇 |
2004年 | 4篇 |
2003年 | 4篇 |
2002年 | 5篇 |
2001年 | 6篇 |
2000年 | 5篇 |
1999年 | 5篇 |
1998年 | 14篇 |
1997年 | 20篇 |
1996年 | 22篇 |
1995年 | 18篇 |
1994年 | 14篇 |
1993年 | 10篇 |
1992年 | 4篇 |
1991年 | 2篇 |
1990年 | 13篇 |
1989年 | 19篇 |
1988年 | 13篇 |
1987年 | 11篇 |
1986年 | 3篇 |
1985年 | 4篇 |
1984年 | 1篇 |
1983年 | 3篇 |
1982年 | 7篇 |
1981年 | 5篇 |
1979年 | 3篇 |
1978年 | 3篇 |
1977年 | 4篇 |
1976年 | 3篇 |
1975年 | 5篇 |
1959年 | 1篇 |
排序方式: 共有372条查询结果,搜索用时 15 毫秒
41.
Michele Pier Luca Guarino Rosa Sessa Annamaria Altomare Silvia Cocca Marisa Di Pietro Simone Carotti Giovanna Schiavoni Rossana Alloni Sara Emerenziani Sergio Morini Carola Severi Michele Cicala 《Digestive and liver disease》2013,45(12):1011-1016
Background
Impairment of gastrointestinal motility is frequently observed in patients with severe infection.Aim
To assess whether exposure of human colonic mucosa to pathogenic lipopolysaccharide affects smooth muscle contractility.Methods
Human colonic mucosa and submucosa were sealed between two chambers, with the luminal side facing upwards and covered with Krebs solution, with or without lipopolysaccharide from a pathogenic strain of Escherichia coli (O111:B4; 1000 ng/mL), and with the submucosal side facing downwards into Krebs. The solution on the submucosal side was collected following 30-min mucosal exposure to Krebs without (N-undernatant) or with lipopolysaccharide (lipopolysaccharide undernatant). Undernatants were tested for lipopolysaccharide and hydrogen peroxide levels and for their effects on smooth muscle cells in the presence of catalase, indomethacin or MG132.Results
Smooth muscle cells incubated with N-undernatant had a maximal contraction of 32 ± 5% that was reduced by 62.9 ± 12% when exposed to lipopolysaccharide undernatant. Inhibition of contraction was reversed by catalase, indomethacin and MG132. Lipopolysaccharide levels were higher in the lipopolysaccharide undernatant (2.7 ± 0.7 ng/mL) than in N-undernatant (0.45 ± 0.06 ng/mL) as well as hydrogen peroxide levels (133.75 ± 15.9 vs 82 ± 7.5 nM respectively).Conclusions
Acute exposure of colonic mucosa to pathogenic lipopolysaccharide impairs muscle cell contractility owing to both lipopolysaccharide mucosal translocation and production of free radicals. 相似文献42.
Normal human platelets were shown to contain the enzyme dihydropteridine reductase. The enzyme was not found in a variety of other cells of hematogenous origin. Partial purification and kinetic and physical data indicated that the platelet enzyme is similar to that previously characterized from liver. Dihydropteridine reductase is important for the regeneration of tetrahydrobiopterin, a required cofactor in hydroxylation reactions involved in biogenic amine formation. The presence of the enzyme may indicate that some synthesis de novo of serotonin and/or catecholamines occurs in platelets, as opposed to a purely storage and transport function. In addition, screening for hyperphenylalaninemia due to dihydropteridine reductase deficiency may become feasible by assaying platelets for enzyme activity. 相似文献
43.
44.
Chondromalacia patellae: assessment with MR imaging 总被引:10,自引:0,他引:10
Yulish BS; Montanez J; Goodfellow DB; Bryan PJ; Mulopulos GP; Modic MT 《Radiology》1987,164(3):763-766
Magnetic resonance (MR) images of the posterior patellar hyaline articular cartilage were obtained in 23 subjects to determine if MR imaging could accurately demonstrate the patellar cartilage. Arthroscopy was used as the standard of reference. Three subjects were asymptomatic volunteers. In the remaining 20 who had patellofemoral pain, arthroscopy was performed before MR imaging in seven and afterward in 12; one did not undergo arthroscopy. MR imaging showed focal areas of swelling of the patellar cartilage, focal hypointensity, surface irregularity, areas of thinning, and areas of cartilage loss with exposure of subchondral bone. The surgical findings agreed with those from MR images in all seven patients who underwent arthroscopy before MR imaging and in ten of the 12 who underwent surgery afterward. MR imaging is an accurate means of examining the posterior patellar cartilage and should be considered as an alternative to diagnostic arthroscopy when chondromalacia patellae is suspected. 相似文献
45.
Breath-hold, contrast-enhanced, three-dimensional MR angiography 总被引:22,自引:0,他引:22
46.
以柱切换HPLC(CSHPLC)技术,对炔诺酮α,β-聚(3-羟丙基)-DL天冬酰胺在家兔体内释放液中炔诺酮的血药浓度进行了测定,以自制ODS预柱,水为流动相联机净化、富集样品;以Shim-packCLC-ODS为分析柱,甲醇-水(7:3)为流动相。结果表明CSHPLC对炔诺酮血药浓度有良好的选择性,回收率为82.6%(n=5),日内和日间精密度(RSD)分别小于4%和6%。在血清0.22~1.10μg/m1范围内炔诺酮色谱峰面积与浓度有较好的线性关系(n=5,r=0.998). 相似文献
47.
48.
49.
Thirty-eight infected pancreatic fluid collections in 23 patients with acute or chronic pancreatitis were drained percutaneously following initial diagnosis with computed tomography and fine-needle aspiration. Fifteen (65.2%) patients were cured completely without surgery. Eight (34.8%) patients required some type of surgery despite successful treatment of the fluid collection, and in two (6.5%) the collection recurred after catheter removal. Complications occurred in three (13%) patients, but only one complication (4%), empyema, was a direct result of catheter drainage. Catheter drainage time averaged 29 days for 16 patients with isolated collections and 96 days and 104 days for patients with collections with pancreatic duct fistulas (nine patients) or gastrointestinal fistulas (14 patients), respectively. This study confirms that infected pancreatic fluid collections can be safely and effectively treated with percutaneous catheter techniques in most patients. 相似文献
50.