分析膝关节镜引导微创复位内固定术对胫骨平台骨折(FTP)患者术后骨折愈合时间及关节功能恢复的影响

目的：分析胫骨平台骨折患者应用膝关节镜引导微创复位内固定术的有效性,以及对术后骨折愈合时间及关节功能恢复的影响。方法：选入我院于2016年1月到2021年12月期间收治的胫骨平台骨折患者120例,以抽签法的形式将其分：60例切开组,60例关节镜组。切开组行常规切开复位固定手术治疗,关节镜组行膝关节镜引导微创复位内固定术治疗,对比两组患者膝关节功能恢复效果、不同时间段两组疼痛评分、两组骨折愈合时间,术后下床活动时间,手术中出血量,切口长度,两组患者治疗效果、不良反应。结果：①关节镜组膝关节恢复总优良率91.67%高于切开组66.67%,具有统计学意义（X2=11.3684,P<0.001）。②手术结束当天,关节镜组疼痛评分为：（8.95±0.76）,切开组（8.86±0.68）,经过对比组间无差异（T=0.6835,P=0.4956）;两组疼痛程度评分无差异（P>0.05）;手术后第四周、十二周的关节镜组疼痛评分分别为（4.42±0.55）、（1.09±0.27）,低于切开组的（6.03±0.58）、（2.40±0.55）,组间存有差异（X2=15.0621,P<0.001;X2=16.5615,P<0.001）。③ 关节镜组骨折愈合时间（3.20±0.30）月,术后下床活动时间（3.65±1.47）d,手术中出血量（79.06±6.65）ml,切口长度(7.20±2.98)cm均优于切开组（5.60±0.77）月,（7.20±2.29）d、（99.67±7.42）ml、(15.44±4.71）cm,组间具有差异（T=22.4961;P<0.001;T=10.1051;P<0.001;T=16.0223;P<0.001;T=11.4517;P<0.001）。④ 关节镜组出现的不良反应3.33%低于切开组25.00%,组间具有差异（X2=11.5820,P<0.001）。结论：治疗胫骨平台骨折采用膝关节镜引导微创复位内固定术,可以提高整体治疗效果,缩短骨折愈合时间,有助于关节功能恢复,值得在临床上推广和应用。     

婚姻质量与人格特质的相关分析

目的　探究夫妻人格特质与婚姻质量的相关以及夫妻间人格特质的相关性 ,为婚姻咨询提供帮助。方法　用 OL-SON婚姻质量问卷 ( ENRICH)和艾森克人格问卷 ( EPQ) ,对 1 0 0对夫妻进行测试和调查。结果　精神质特质、神经质特质与婚姻质量总分存在极显著负相关 ( P<0 .0 0 1 ) ,掩饰维度与婚姻质量总分存在极显著正相关 ( P<0 .0 0 1 ) ,精神质和神经质均与婚姻质量的 8个因子存在极显著负相关 ( P<0 .0 0 1 ) ,丈夫的神经质与妻子精神质特质间存在显著性正相关 ( P<0 .0 1 ) ,与妻子的掩饰维度存在显著性负相关 ( P<0 .0 5 )。结论　婚姻质量与人格关系极为密切。     

双性化对大学生主观幸福感的影响

目的揭示双性化对大学生主观幸福感的影响。方法采用性别角色量表和主观幸福感量表对4所不同类型院校大学生的性别角色取向及主观幸福感进行了测评。结果大部分学生的性别角色趋于双性化,但男女同学趋于双性化的程度有所不同,男女大学生不同的男性化和女性化程度对主观幸福感的影响也不同。结论不论男女,男性化取向的大学生在生活满意度和总体幸福感上的得分高一些;对男生而言,双性化取向的男生在情感维度上的得分高一些;对女生而言,男性化水平高的女生在情感维度上的得分高一些。     

神经调节因子及其信号转导机制

神经调节因子(NRG)是一个由4种基因编码的多肽家族,包含4种同源异构体NRG-1,2,3和4,其功能性受体是由ErbB酪氨酸激酶受体组成,属于跨膜酪氨酸激酶的表皮生长因子受体家族成员,包括ErbB2/HER2/neu,ErbB3/HERS和ErbB4/HER4.NRG通过诱导ErbB受体构象变化,使ErbB蛋白形成二聚体,继而激活酪氨酸激酶,引起C-末端的自身酪氨酸磷酸化和反式酪氨酸磷酸化,而发挥其生物学作用.     

Preparation and characterization of porous beta-tricalcium phosphate/collagen composites with an integrated structure

Porous beta-tricalcium phosphate (TCP)/collagen composites with different beta-TCP/collagen weight ratio were prepared. The influences of the preparation conditions on the microstructure of porous composite and the joint status of beta-TCP particles with collagen fibrils were characterized by X-ray diffractometer, scanning electron microscopy and transmission electron microscopy. The results showed: (1) an acid treatment could effectively disassemble collagen fibrils; (2) in the resulting porous composites, beta-TCP particles homogenously existed on the skeleton of the collagen fibril network and bonded tightly to both the fibrils and themselves. The tight bonding formation could be due to the reaction between Ca ions in the particles and carboxyl groups in collagen polypeptide chains and due to the reprecipitation of partially dissolved beta-TCP during synthesis. The tight bonding between beta-TCP particles and collagen fibrils in the composites demonstrated an integrated structure, which was reproducible when beta-TCP/collagen ratio ranged from 2 to 4. Such integrated structure would make significant contributions in reliably tailoring properties of the porous composites by varying beta-TCP content. In addition, the porous composites had large porosity (approximately 95%) and appropriate pore size (approximately 100 microm), showed no negative impact in cytotoxicity assay and complete bone tissue regeneration after 12 weeks in animal test.     

Survey of the Distribution of a Newly Characterized Receptor for Advanced Glycation End Products in Tissues

Advanced glycation end products (AGEs), the final products of nonenzymatic glycation and oxidation of proteins, are found in the plasma and accumulate in the tissues during aging and at an accelerated rate in diabetes. A novel integral membrane protein, termed receptor for AGE (RAGE), forms a central part of the cell surface binding site for AGEs. Using monospecific, polyclonal antibody raised to human recombinant and bovine RAGE, immunostaining of bovine tissues showed RAGE in the vasculature, endothelium, and smooth muscle cells and in mononuclear cells in the tissues. Consistent with these data, RAGE antigen and mRNA were identified in cultured bovine endothelium, vascular smooth muscle, and monocyte-derived macrophages. RAGE antigen was also visualized in bovine cardiac myocytes as well as in cultures of neonatal rat cardiac myocytes and in neural tissue where motor neurons, peripheral nerves, and a population of cortical neurons were positive. In situ hybridization confirmed the presence of RAGE mRNA in the tissues, and studies with rat PC12 pheochromocytes indicated that they provide a neuronal-related cell culture model for examining RAGE expression. Pathological studies of human atherosclerotic plaques showed infiltration of RAGE-expressing cells in the expanded intima. These results indicate that RAGE is present in multiple tissues and suggest the potential relevance of AGE-RAGE interactions for modulating properties of the vasculature as well as neural and cardiac function, prominent areas of involvement in diabetes and in the normal aging process.     

Early embryonic expression patterns of the mouse Flamingo and Prickle orthologues.

The Drosophila melanogaster proteins Flamingo and Prickle act in the planar cell polarity (PCP) pathway, which is required for acquisition of epithelial polarity in the wing, eye, and epidermis. In mammals, PCP signaling has been shown to regulate cell movements and polarity in a variety of tissues. Here, we show that the murine Flamingo orthologues Celsr1-3 and the Prickle orthologues Prickle1, Prickle2, and Testin have dynamic patterns of expression during pregastrulation and gastrulation stages. Celsr1 is expressed in the anterior visceral endoderm and nascent mesoderm, Celsr2 and Celsr3 mark the prospective neuroectoderm, Prickle1 is expressed in the primitive streak and mesoderm, Prickle2 in the node, and Testin in the anterior visceral endoderm, the extraembryonic ectoderm, primitive streak, and mesoderm. Analysis of a gene-trap mutation in Testin indicates that this gene is not required for embryogenesis; therefore, other Prickle homologues may compensate for its function during development.     

携带hIL-2cDNA的逆转录病毒载体的构建及其在人肝癌细胞中的特异表达研究

将人白细胞介素２（ｈＩＬ-２）ｃＤＮＡ克隆到逆转录病毒载体ＭＮＳＭ的ＰＬ位点　，分别构建了转录受ＳＶ４０早期启动子和人甲胎蛋白增强子调控的重组逆转录病毒载体ＭＮＳＩ和ＭＮＳＩＡ。用脂质体转染法将ＭＮＳＩ和ＭＮＳＩＡ分别转导ＰＡ３１７包装细胞，测质粒转染率为（５～２０）×１０~(－３)克隆／μｇＤＮＡ·１０~６细胞，病毒感染率为（５．４～４５０）×１０~４ＣＦＵ／ｍｌ。重组病毒在４μｇ／ｍｌ ｐｏｌｙｂｒｅｎｅ存在条件下感染人肝癌细胞、肾癌细胞和黑色素瘤细胞，Ｎｅｏ~Ｒ克隆经Ｓｏｕｔｈｅｒｎｂｌｏｔ分析证明ｈＩＬ－２ｃＤＮＡ转入人肿瘤细胞并整合，　Ｒ　ＮＡ斑点杂交及ＩＬ－２活性表达分析证明，人甲胎蛋白增强子可促进异源启动子启动ｈＩＬ－２ｃＤ－ＮＡ在合成甲胎蛋白的人肝癌细胞中高效特异转录和表达。该研究对肝癌特异性免疫增强基因治疗有重要意义。