The development and evaluation of a mu-capture ELISA detecting Chlamydia-specific IgM

A mu-capture enzyme-linked immunosorbent assay (ELISA) for detecting chlamydia-specific IgM was developed by use of the heat stable, lipopolysaccharide group-specific antigen and an alkaline phosphatase-labelled anti-chlamydia group-specific monoclonal antibody conjugate. The test was used to study the serological response in chlamydial respiratory tract infection among patients with acute respiratory tract symptoms in Cambridgeshire during the past 7 years. Results were compared with those of the complement fixation test (CFT) in routine use as well as those of a whole inclusion indirect immunofluorescence (WIF) test for IgM. Correlation between results of the mu-capture ELISA and those of the WIF test was 87.5%. The percentage of patients in whom specific IgM was found fell with increasing age. This may be due to lack of recall of IgM as a response to reinfection. Chlamydia-specific IgM was more likely to be detected when the CFT titre was greater than or equal to 64 and was rarely detected more than 6 months after the onset of symptoms. However, several patients less than 20 years of age were found to have specific IgM with CF antibody titres less than 64. We have found the mu-capture ELISA a useful test for the diagnosis of respiratory tract chlamydial infections, particularly in younger patients.     

Post-keratoplasty corneal ulcers and bandage lenses

285 cases of penetrating keratoplasty in whom hydrophilic bandage lenses were fitted were analyzed for the occurrence of corneal ulcers. Of these eyes 26 developed culture-proven bacterial ulcers. No correlation could be made between any particular type of contact lens and the development of corneal ulcer. Although no single factor could be delineated which may be responsible for this problem, this risk appears to be escalated where host defense mechanisms are impaired. The clinician should recognize the potential risk in using bandage lenses when the cornea is immunosuppressed and completely denervated.     

Adult body size and survivorship in a field population of Aedes triseriatus

Aedes triseriatus pupae, host-seeking, and ovipositing females were collected at a southern Wisconsin site endemic for La Crosse encephalitis virus. The host-seeking and ovipositing females were age-graded by the Detinova and Polovodova techniques, respectively. Wing lengths were determined for all mosquitoes. Wing lengths of the mosquitoes collected as pupae were predicted from pupal weight by a simple regression equation. There was significant seasonal variation in body size of adult females, but no strong evidence that increased size was advantageous to survival. It is concluded therefore that the potential length of infective life is not affected by the size of the adult female.     

Increasing evidence of the rise of cancer in workers exposed to vinylchloride.

The results of a cancer mortality study among workers employed in the production of vinylchloride and polyvinylchloride between 1939 and 1977 suggest a significant increase in deaths from malignancies of the lymphatic and haemopoietic tissues. Mortality for tumours of the digestive organs, respiratory system, bone and connective tissues, brain, and skin are also greater than in the general population. There were no registered cases of liver angiosarcoma in the study cohort during the follow up period. The risk of cancer was highest among the workers exposed to concentrations of VC of 300 mg/m3 and more who had worked at the plant for 15 to 19 years. The relatively high number of leukaemias and lymphomas in the study group and the absence of liver angiosarcomas probably reflects specific carcinogenic action of different doses of vinylchloride.     

Optimal Selection of Sib Pairs from Random Samples for Linkage Analysis of a QTL Using the EDAC Test

Percentages of extremely concordant and extremely discordant sib pairs are calculated that maximize the power to detect a quantitative trait locus (QTL) under a variety of circumstances using the EDAC test. We assume a large fixed number of randomly sampled sib pairs, such as one would hope to find in the large twin registries, and limited resources to genotype a certain number of selected sib pairs. Our aim is to investigate whether optimal selection can be achieved when prior knowledge concerning the QTL gene action, QTL allele frequency, QTL effect size, and background (residual) sib correlation is limited or absent. To this end we calculate the best selection percentages for a large number of models, which differ in QTL gene action allele frequency, background correlation, and QTL effect size. By averaging these percentages over gene action, over allele frequency, over gene action, and over allele frequencies, we arrive at general recommendations concerning selection percentages. The soundness of these recommendations is subsequently in a number of test cases.     

The use of perfluorinated emulsion for prolongation of the endurance period in lethal hypoxia

The apnea model with venovenous perfusion and blood oxygenation in a membrane oxygenator was used to study the gas transport characteristics of perfluorinated emulsion with the aim to prolong the endurance period in lethal hypoxia. The use of PFOS emulsion (40 ml/kg) as a hemodilution agent at relatively low rate of assisted perfusion (35 ml/kg×min) produced no improvement of oxygen supply during the critical period in comparison with conventional plasma substitutes. However, perfusion with oxygenated perfluorinated emulsion prolonged survival as compared with polyglucin perfusion, mainly due to the maintenance of the vitally important organs (heart and brain) and due to the improvement of microcirculation. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 124, No. 10, pp. 477–480, October, 1997