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21.
Yanagi D Shirai K Arimura T Saito N Mitsutake C Mitsutake R Hida S Iwata A Nishikawa H Kawamura A Miura S Saku K 《Internal medicine (Tokyo, Japan)》2008,47(20):1803-1805
We describe the case of an 85-year-old woman in whom pericardiocentesis, prolonged bed rest and blood pressure control were performed without surgery to successfully treat an oozing-type myocardial rupture due to myocardial infarction. 相似文献
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Matsushima Y Kinoshita Y Fukui H Maekawa T Yazumi S Okada A Nakase H Kawanami C Iwano M Hashimoto K Takeda Z Okazaki K Chiba T 《Journal of gastroenterology》2002,37(6):428-433
Background:
Background: Although Helicobacter pylori eradication is effective in treating low-grade gastric mucosa-associated lymphoid tissue (MALT) lymphoma, the condition in
some patients deteriorates even after the eradication. Therefore, it is important to predict the disease outcome before starting
H. pylori eradication. We investigated the usefulness of flow cytometry, quantifying CD19- and CD20-positive B lymphocytes in MALT lymphoma
tissue, for predicting the disease outcome after H. pylori eradication.
Methods: Tissue specimens from 14 patients with H. pylori-positive low-grade gastric MALT lymphoma were examined by histology, Southern blotting, and flow cytometry before therapy.
Serum levels of soluble interleukin (IL)-2 receptor were also measured. The relationship between the data and the prognosis
after H. pylori eradication was analyzed.
Results: Remission occurred in 10 of the 14 patients. The condition in the 4 remaining patients deteriorated even after H. pylori eradication. The percentages of CD19- and CD20-positive cells in MALT lymphoma tissue from the patients in remission were
both significantly lower than those in the tissue from patients not in remission. Indeed, 4 of the 5 patients in whom both
CD19- and CD20-positive cells accounted for more than 50% of the total number of lymphocytes had gastrectomy, whereas all
patients in whom both CD19- and CD20-positive cells accounted for less than 50% of the total number of lymphocytes achieved
remission. Although immunoglobulin gene rearrangement was present in all patients operated on, there were also 6 patients
whose MALT lymphoma was ameliorated in spite of the presence of gene rearrangement. The serum level of soluble IL-2 receptor
was in the normal range in all patients tested.
Conclusions: Analysis of mature B-cell markers in MALT lymphoma tissue is more useful than the examination of immunoglobulin gene rearrangement
or serum levels of soluble IL-2 receptor in predicting the outcome of low-grade gastric MALT lymphoma after H. pylori eradication.
Received: January 5, 2001 / Accepted: November 2, 2001 相似文献
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OBJECTIVE: The receptor tyrosine kinase Flk-2/Flt-3 (Flt-3) represents an important molecule involved in early hematopoiesis. Murine hematopoietic stem cells (HSCs) have been shown to be negative for the expression of Flt-3. We now present clear evidence for the expression change of Flt-3(-) HSCs in an activating state, and the reversibility of Flt-3 expression by HSCs in vivo. MATERIALS AND METHODS: Bone marrow cells isolated from Ly5.1 mice were sorted on the basis of Flt-3 expression and transplanted into lethally irradiated Ly5.2 recipients. After 24 weeks, peripheral blood was analyzed for donor contribution by flow cytometry. RESULTS: Although long-term engraftment was predominantly detected in Flt-3(-) populations as previously described, a 6-day cultivation of Lin(-/low)c-kit(+)Sca-1(+) Flt-3(-) bone marrow cells with stem cell factor and interleukin-11 resulted in the generation of Flt-3(+) HSCs with long-term engraftment capabilities. However, the Flt-3 ligand had no significant effect on self-renewal of the Flt-3(+) HSCs. Next, to examine reversible expression of this receptor molecule, Flt-3(+) cells converted in vitro from Ly5.1 Lin(-/low)c-kit(+)Sca-1(+) Flt-3(-) bone marrow cells were isolated and transplanted into Ly5.2 primary recipients. After 24 weeks, Ly5.1 Lin(-/low) bone marrow cells were again separated into Flt-3(-) and Flt-3(+) cells and retransplanted into Ly5.2 secondary recipients. The majority of donor HSCs with long-term engraftment capabilities were detected in the Flt-3(-) populations, indicating the reversion of Flt-3(+) to Flt-3(-) HSCs. CONCLUSIONS: These observations suggest that Flt-3 is a useful cell-surface marker of HSC activation and that this phenotypic change is reversible. 相似文献
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Kazuhiro OSANAI Chiharu TSUCHIHARA Rieko HATTA Taku OIKAWA Katsuma TSUCHIHARA Masaharu IGUCHI Toshimitsu SEKI Masakatsu TAKAHASHI Jyongsu HUANG Hirohisa TOGA 《Respirology (Carlton, Vic.)》2006,11(S1):S70-S73
Abstract: Pulmonary surfactant (PS) is a mixture of several lipids (mainly phosphatidylcholine; PC) and four apoproteins (A, B, C and D). The classical hypothesis of PS transport suggests that PS is synthesized in the endoplasmic reticulum and transported to the lamellar body (LB) via the Golgi apparatus. However, recent studies have raised questions regarding this single route. This study examined, independently, the intracellular trafficking route of three different components of PS, that is, PC, SP-A and SP-B. Alveolar type II cells were isolated from Sprague–Dawley rats or Japanese white rabbits. The cells were cultured with either [3 H]choline or [35 S]methionine/cysteine with or without brefeldin A, which disassembles the Golgi apparatus. LB was purified from disintegrated cells with sucrose density gradient centrifugation. [3 H]PC was extracted from radiolabeled media, cells, and the LB fraction with Bligh–Dyer's method. [35 S]SP-A or [35 S]SP-B was immunoprecipitated from each sample with a specific antibody. [3 H]PC was transported and stored to the LB via a Golgi-independent pathway. [35 S]SP-A was transported to the Golgi apparatus, underwent glycosylation, and was then constitutively secreted. The secreted [35 S]SP-A was re-uptaken into the LB. [35 S]SP-B was transported and stored to the LB via the Golgi-dependent pathway. These results indicate that, rather than a single route, surfactant components take different pathways to reside in the LB. These different pathways may reflect the different nature and role of each surfactant component such as surface tension-lowering activity and innate host defense. 相似文献
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