论医药卫生法学专业学生实务能力的培养

加强医药卫生法学专业学生实务能力的培养,是实现医药卫生法学专业培养目标的需要.在教学中,通过运用案例教学法、讨论式教学法,开展模拟法庭、第二课堂活动,组织临床见习、毕业实习等途径,培养即有法律知识又有医药卫生知识的复合型人才.     

金地鼠颊囊癌前病变的细胞增殖动力学研究

目的研究口腔上皮组织癌和癌前病变的细胞动力学变化特征。方法采用Ｓａｌｅｙ的ＤＭＢＡ涂布诱导法，建立金地鼠颊囊癌前病变模型，并用溴脱氧尿苷流式细胞动力学检测方法，对３０只叙利亚种金地鼠进行分组对照研究。结果涂药组光镜下的细胞病变程度随涂药时间延长而逐渐加重，而空白对照组无变化；ＦＣＭ检测显示涂药组与对照组在Ｓ期阳性细胞检出率存在显著差异。即使在光镜下表现为轻度不典型增生时，ＦＣＭ检测结果也有显著差别。结论金地鼠颊囊癌前病变具有细胞异常增殖的细胞动力学特征     

Transforming Growth Factor-β1 Induces Apoptosis through Down-Regulation of c-mycGene and Overexpression of p27Protein in Cervical Carcinoma

Transforming growth factor-β1 (TGF-β1) is known to be a potent growth inhibitor for many cell types, including most epithelial cells. In skin keratinocytes, TGF-β1 has been shown to inhibit growth and to rapidly reduce c-mycexpression. However, the molecular mechanism of TGF-β1 action on cell growth of cervical carcinoma has not yet been elucidated. We thus assessed the effect of TGF-β1 on the growth of cervical carcinoma cell lines. Two cervical squamous carcinoma cell lines, CUMC-3 and CUMC-6, were incubated with varying concentrations of TGF-β1, and growth inhibition was evaluated with tetrazolium-based colorimetric assay. After culture in TGF-β1 for 24 h, inhibition of growth was detected in a dose-dependent manner at concentrations of 0.1–10 ng/ml in both cell lines. This effect of TGF-β1 on cultured carcinoma cells was associated with apoptotic process including oligonucleosomal ladder DNA and apoptotic body formations. Northern blot analysis revealed c-mycmRNA expression was suppressed by 10 ng/ml of TGF-β1 following 3 h of treatment in both cell lines. Western blot analysis showed that the level of p27^Kip1protein was increased after TGF-β1 treatment in both cell lines. These results suggest that the mechanisms by which TGF-β1 inhibits the growth of cervical carcinoma are complex and may include effects on down-regulation of c-mycgene, and overexpression of p27^Kip1protein.     

Construction of retroviral vectors to induce a strong expression of human interferon-β gene in human hepatoma cells

ClassIinterferonisapotent1mmunemodu-latingfactorinvivo,andplaysanimportantrolelnactivatinganti-tumorcytotoxicTlymphocytesandnonuspecificeffectorcellssuchasNKcellsLlJ.ln-terferonandmanyothercytokinegeneswereintro-ducedintotumorcells,tumorinterstitialcellsandtumorinfiltratinglymphocytes['-']inordertosustainaneffectiveanti-tumorconcentrationofcy-tokinelocatedattumorsites.Itwasshownthatsomecytokinegenemodifiedtumorcellslosttheirtumorigenicityandinhibitedthegrowthofparentaltumorinanimalmodels.Pra…     

Study of the role of vitamin B12 and folinic acid supplementation in preventing hematologic toxicity of zidovudine

Abstract: A prospective, randomized study was conducted to evaluate the role of vitamin B₁₂ and folinic acid supplementation in preventing zidovudine (ZDV)-induced bone marrow suppression. Seventy-five human immunodeficiency virus (HIV)-infected patients with CD4 + cell counts < 500/mm³ were randomized to receive either ZDV (500 mg daily) alone (group I, n = 38) or in combination with folinic acid (15 mg daily) and intramascular vitamin B₁₂ (1000 μg monthly) (group II, n = 37). Finally, 15 patients were excluded from the study (noncompliance 14, death 1); thus, 60 patients (31 in group I and 29 in group II) were eligible for analysis. No significant differences between groups were found at enrollment. During the study, vitamin B₁₂ and folate levels were significantly higher in group II patients; however, no differences in hemoglobin, hematocrit, mean corpuscular volume, and white-cell, neutrophil and platelet counts were observed between groups at 3, 6, 9 and 12 months. Severe hematologic toxicity (neutrophil count < 1000/mm³ and/or hemoglobin < 8 g/dl) occurred in 4 patients assigned to group I and 7 assigned to group II. There was no correlation between vitamin B₁₂ or folate levels and development of myelosuppression. Vitamin B₁₂ and folinic acid supplementation of ZDV therapy does not seem useful in preventing or reducing ZDV-induced myelotoxicity in the overall treated population, although a beneficial effect in certain subgroups of patients cannot be excluded.     

一起阴囊皮炎爆发流行的调查与实验研究

本文报道了一起183例农民阴囊皮炎的爆发流行,发病率高达56.63%,不同的年龄、职业与发病率无显著差别,八月份为发病高峰。同时患有口角炎、舌炎并发症者占45.65%,取14例典型患者的阴囊鳞屑作真菌镜检,检出霉菌1例。85例患者经采用核黄素10mg,加复合维生素B10mg,每日两次连服七天进行试治,治愈率为96.47%(82/85),将19例患者与20例健康者对照,作4h尿中核黄素负荷试验比较,结果两者在统计学上具有显著性差别(u=2.11,P≤0.05)。经膳食营养调查,发现6~10月份核黄素供给不足47%。由此证明,发病与核黄素缺乏有关。     

特发性甲状旁腺功能低下（附13例分析）

本文观察分析了13例特发性甲状旁腺功能低下患者、查PTH6例。患者血清中除有本病低血钙、高血磷的固有特征外，其它微量元素也有改变，如血清镁值减低。这与文献[1]的报道相符。碱性磷酸酶2例明显升高。磷廓清率S例降低。肾小管磷重吸收率5例升高。放免甲功：抗甲状腺微粒体2例升高。T细胞亚群1例低于正常值。本病有家族性，与遗传有关。