一DXFD52相关人肝细胞cDNA的部分序列分析及其反转录病毒载体的构建

为了寻找和研究新的人类基因ｃＤＮＡ，本实验以Ｔ７ＤＮＡ聚合酶对一ＤＸＦＤ５２相关人肝细胞ｃＤＮＡ（ＤＥ）进行了分段部分测定，并将所测各部分序列分别在ＥＭＢＬ（欧洲分子生物学库）中进行核酸同源性检索，结果在库中没有找到任何具有同源性的人类基因或ＤＮＡ片段。因此，我们初步认为ＤＥ为一新的人类基因ｃＤＮＡ片段。同时为初步探讨ＤＥ的功能，我们还成功地将ＤＥ构建于反转录病毒载体ＰＬＸＳＮ上。     

Human monocyte-derived hemangioma-like endothelial cells: evidence from an in vitro study

BACKGROUNDS: Hemangiomas are highly prevalent in newborns and infants and can lead to severe complications. However, the pathogenesis of hemangiomas is still unknown. This study was designed to examine the potential of human monocytes to differentiate into hemangioma endothelial cells. METHODS: Purified monocytes from adult human peripheral blood were cultured under a conditional culture environment supplemented with basic fibroblast growth factor and vascular endothelial growth factor. Cells cultured for 2 weeks were subjected to histological and immunochemical examinations in order to determine the expression of specific markers for hemangioma endothelial cells. RESULTS: Monocytes cultured for 2 weeks in angiogenic medium expressed human erythrocyte-type glucose transporter protein, FcgammaRII, and several other endothelial markers, all of which are deemed specific markers for hemangioma endothelial cells. However, neither CD133 nor alpha smooth muscle actin was detected in our monocyte culture. CONCLUSION: Our data suggested that monocytes are capable of differentiating into hemangioma endothelial cells under the angiogenic stimulation from microenvironment of proliferative hemangioma.     

Pretreatment with Tongxinluo protects porcine myocardium from ischaemia/reperfusion injury through a nitric oxide related mechanism

Background The traditional Chinese medicine Tongxinluo can protect myocardium against ischaemia/reperfusion injury, but the mechanism of its action is not well documented. We examined the involvement of nitric oxide in the protective role of Tongxinluo. Methods Miniswine were randomized to four groups of seven: sham, control, Tongxinluo and Tongxinluo coadministration with a nitric oxide synthase inhibitor Nω-nitro-L-arginine (L-NNA, 10 mg/kg i.v.). Three hours after administration of Tongxinluo, the animals were anaesthetised and the left anterior descending coronary artery ligated and maintained in situ for 90 minutes followed by 3 hours of reperfusion before death. Area of no reflow and necrosis and risk region were determined pathologically by planimetry. The degree of neutrophil accumulation in myocardium was obtained by measuring myeloperoxidase activity and histological analysis. Myocardial endothelial nitric oxide synthase activity and vascular endothelial cadherin content were measured by colorimetric method and immunoblotting analysis respectively.Results Tongxinluo significantly increased the local blood flow and limited the infarct and size of no reflow. Tongxinluo also attenuated myeloperoxidase activity and neutrophil accumulation in histological sections and maintained the level of vascular endothelial cadherin and endothelial nitric oxide synthase activity in the reflow region when compared with control group. The protection of Tongxinluo was counteracted by coadministration with L-NNA. Conclusions Tongxinluo may limit myocardial ischaemia and protect the heart against reperfusion injury. Tongxinluo regulates synthesis of nitric oxide by altering activity of endothelial nitric oxide synthase.     

Autologous peripheral blood stem cell transplantation in the patients with hematologic malignancies and solid tumors

Objective To evaluate the long-term therapeutic effects of autologous peripheral blood st em cell transplantation (auto-PBSCT) on the treatment of hematological and soli d tumors. Methods Fifty-one patients were recruited in this auto-PBSCT study, in which several p otentially important parameters were studied including the optimal time for stem cell co llection, the dose of stem cell reinfusion, the time of hematopoietic reconsti tution, the disease free survival (DFS) and overall survival (OS), complication s related to transplantation, and maintenance chemotherapy after auto-PBSCT. Results After APBSCT, 3-year and 5-year survival rates of NHL were 83.3%; those of AM L were 74.7%; those of MM were 37.9% and 19%; those of ALL were 40% and 0% res pectively. Hematopoietic reconstitution was greatly promoted by granulocyte col ony stimulating factor (G-CSF). The mean time for patients’ neutrophil to reco ver up to >0.5×10(9) /L after APBSCT was 11.14 days in the group of the patien ts receiving G-CSF in contrast to 17.6 days in the group receiving no G-CSF. The most common complications of transplantation were fever, liver dysfunction and hypokalaemia, which were curable. No death was due to transplantation related complications.Conclusion Comparing with conventional chemotherapy, our study suggests that auto-PBSCT i s a very important therapeutic option that can significantly improve the prognos is in the patients with hematological and solid tumors, especially in the patien ts with AML and NHL.     

5 Gy全身照射对大鼠伤口巨噬细胞的抑制效应及W11-a12的促愈作用

目的 研究大鼠全身5Gy照射对伤口巨噬细胞（MΦ）功能的影响及W11－a12的促愈作用。方法 采用^60Coγ射线身身照射复合皮肤创伤模型,观察了MΦ酵母－补体复合物玫瑰花环形成试验、吞噬功能和分泌纤维粘连蛋白（FN）、转化生长因子β1（TGFβ1）、碱性成纤维细胞生长因子（bFGF）的功能。结果 大鼠5Gy全身照射复合皮肤创伤后第3、5天伤口内巨噬细胞C3b受体表达、吞噬功能,合成与分泌FN、TGFβ、bFGF的功能均显著受到抑制,但抑制效应的程度不同,MΦ的吞噬功能的抑制重于其分泌功能,分泌TGFβ1功能的抑制重于分泌bFGF的功能,W11-a12能显著逆转辐射对MΦ功能的抑制效应。结论 伤口MΦ功能受抑是合并放射损伤后伤口难愈的主要原因,增强MΦ功能是W11－a12促愈作用的一个途径。     

三味中药配伍应用对改善烫伤小鼠受损免疫功能的作用

目的　观察对烫伤小鼠受损免疫功能具有调理作用的单味中草药 ,经过配伍应用后的协同作用。方法　采用 1 .0 3 4× 1 0 5 Pa的蒸气烫伤 1 0s ,烫伤面积 1 6% ,深度为Ⅲ度的致伤模型烫伤动物。通过观察MΦ的吞噬功能、枯否细胞清除能力、MΦ抗原提呈功能、血清TNF量、淋巴细胞增殖反应及IL 2产生能力、小鼠抗体产生能力、PMNH2 O2 释放量及PMN溶菌酶释放量、血清补体的趋化作用和聚集作用等指标 ,籍以了解单味中药与配伍中药对烧 (烫 )伤小鼠受损免疫功能的调理作用。结果　单味中药三七(PP) ,金银花 (LJ)和虎杖 (PC)对小鼠受损免疫功能有一定的改善作用。PC +LJ、PP +PC、PP +LJ等二味配伍的中药 ,除少数指标外 ,均比单味药的作用好。而PP +LJ+PC三味配伍药的调理作用 ,比单味药和二味配伍药的调理作用更佳。结论　单味中药经配伍应用后对烧 (烫 )伤小鼠严重受损的免疫系统功能 ,具有比单味药更好的协同调理作用。     

偏头痛患儿的大脑血流动力学变化

目的　研究偏头痛患儿的脑动脉血流动力学改变 ,探讨其在小儿偏头痛发生中的作用。方法　应用经颅多普勒 (TCD)技术 ,检测 90例 7～ 14岁头痛间歇期的偏头痛患儿和 80例健康同龄儿童脑动脉血管血流动力学指标。结果　 90例偏头痛患儿中脑动脉血流动力学异常者 77例 (86 % ) ,其中 6 0例 (6 7% )流速增快 ,9例 (10 % )减慢 ,与对照组相比 ,差异具有显著性 (P <0 0 1) ;90例中 2 3例 (2 6 % )存在双侧同名动脉血流速度不对称 ,其中伴有血流速度异常者15例 ,血流速度正常者 8例 ,差异具有显著性 (P <0 0 1)。结论　脑动脉血流动力学因素异常改变 ,在小儿偏头痛的发生中具有重要作用。尤其值得注意的是 ,双侧同名动脉流速不对称的改变 ,在小儿偏头痛的发生中同样可能起着重要的作用。     

孤立性蝶窦病变的诊断与治疗

目的;提高孤立性蝶窦病变的诊断和治疗率。方法;总结使用鼻窦内窥镜诊治孤立性蝶窦病变１２例的全过程。结果：１２例孤立性蝶窦病变的患者的均获准确诊断,使用鼻窦镜行经鼻腔蝶窦手术,有效率为８３．３％,随访６￣２４个月,１０例无复发,复发２例二次手术后治愈;结论：孤立性蝶窦病变患者可用鼻窦内窥镜诊断与治疗。     

甘油三酯增高者畸变产物耳声发射的初步观察

目的：观察甘油三酯增高者变畸变产物耳声发射（ＤＰＯＡＥ）的振幅和潜伏期的变化情况。方法：采用ＩＬＯ－Ｖ５耳动态分析仪检测３０例（５５耳）甘油三酯增高者和１８例（３６耳）正常人的ＤＰＯＡＥ的振幅和潜伏期。结果：本组病例虽无纯音听阈异常,但ＤＰＯＡＥ振幅明显下降,而潜伏振幅值和明显。结论：甘油三酯增高者耳蜗功能可能有早期的损害,ＤＰＯＡＥ的反应幅值和潜伏期在产生机制和影响因素方面有所差异。     

喉癌微血管生成中P53 nm23蛋白和血管内皮生长因子的作用

目的　探讨P5 3、nm2 3蛋白和血管内皮生长因子 (vascularendothelialgrowthfacor,VEGF)在喉癌微血管生成及转移中的作用。方法　通过免疫组化SP法对 42例喉癌标本中P5 3、nm2 3蛋白、VEGF及微血管密度 (microvesseldensity,MVD)进行了检测。结果　喉癌中P5 3、nm2 3蛋白及VEGF的阳性表达率分别占 47 6 %、5 7 1%和 71 4%。P5 3基因和VEGF呈正相关 (P <0 0 5 ) ,在有淋巴结转移的喉癌标本中P5 3、VEGF阳性表达率及MVD明显高于非转移组 (P <0 0 5 )。而nm2 3基因和VEGF在喉癌标本中无直接相关性 ,在nm2 3蛋白表达阴性和VEGF阳性标本中MVD较高 ,这种现象多见于有淋巴结转移的喉癌中。结论　突变型P5 3基因通过调控VEGF的表达影响肿瘤内MVD ,促使喉癌发生转移 ;而nm2 3基因可能不是通过调控VEGF的表达 ,而是通过其它途径影响喉癌转移。