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31.
32.
Mutations in the retinal guanylate cyclase (RETGC-1) gene in dominant cone-rod dystrophy 总被引:3,自引:0,他引:3
Kelsell RE; Gregory-Evans K; Payne AM; Perrault I; Kaplan J; Yang RB; Garbers DL; Bird AC; Moore AT; Hunt DM 《Human molecular genetics》1998,7(7):1179-1184
The dominant cone-rod dystrophy gene CORD6 has previously been mapped to
within an 8 cM interval on chromosome 17p12-p13. The retinal- specific
guanylate cyclase gene (RETGC-1), which maps to within this genetic
interval and previously was implicated in Leber's congenital amaurosis, was
screened for mutations within this family and in a panel of small families
and individuals with various cone and cone- rod dystrophy phenotypes. A
missense mutation (E837D) was identified in affected members of the CORD6
family, as well as a second missense mutation (R838C) in three other
families with dominant cone-rod dystrophy. RETGC-1 is only the fourth gene
to be implicated in cone-rod dystrophy and this is the first report of
dominant mutations in this gene.
相似文献
33.
There are advantages and disadvantages associated with utilization of online health services among individuals living with cancer. Accessing accurate, reliable health-related information online gives patients the power to enhance their understanding of information they obtain from their health care providers. However, online health information can often be confusing for patients to interpret, and it can sometimes be conflicting or incorrect. Based on a framework by Eysenbach, the following paper discusses various types of cancer services that are available online, and it addresses both positive and negative health outcomes that have been linked to utilizing such services. 相似文献
34.
Pincivero DM Coelho AJ Campy RM Salfetnikov Y Bright A 《European journal of applied physiology》2002,87(4-5):448-455
The purpose of this study was to examine the effect of voluntary contraction efforts on the median frequency (f
med) of the electromyogram (EMG) recorded from the quadriceps femoris muscle in healthy men and women. A group of 30 healthy
volunteers (15 men, 15 women) were assessed for EMG activity of the vastus medialis (VM), vastus lateralis (VL), and rectus
femoris (RF) muscles during isometric contractions with the knee at 60° flexion. Subjects performed a series of 5 s maximal
voluntary isometric contractions that anchored the perceptual range with a "10" on a 10-point scale. Sub-maximal isometric
contractions were then separately performed at the following perceived effort levels on the 10-point scale: 1, 2, 3, 4, 5,
6, 7, 8 and 9, in a random order. Subjects were instructed to maintain the contraction at each perceived level of effort for
5 s. The f
med of the three muscles was assessed using a power spectrum analysis performed over 11 consecutive, 512 ms, epochs overlapping
each other by half their length during the middle 3 s of each contraction. The f
med for each of the 11 epochs was then determined for each muscle, followed by calculation of the means and normalized coefficients
of variation [(standard deviation/mean)×100%] for each contraction. The results demonstrated that the mean f
med of VL was significantly greater than those of the other two muscles, and that f
med of RF was significantly greater than that of VM. The VL muscle demonstrated a significant increase in mean f
med across the contraction efforts, compared to the VM and RF muscles that displayed a significant decrease. The men displayed
significantly higher f
med values for the VM muscle than did the women, as well as showing a significantly greater increase across the contraction efforts
for the VL muscle. The variability of f
med was shown to be significantly higher for the VM muscle, compared to the VL and RF muscles. The findings of this study suggest
that the f
med statistic is most sensitive to contraction intensity efforts for the VL muscle, and that men display significantly higher
values for the VL and VM muscles, compared to women.
Electronic Publication 相似文献
35.
Detection of cytomegalovirus infection during a vaccine clinical trial in healthy young women: seroconversion and viral shedding. 总被引:3,自引:0,他引:3
Changpin Zhang Hannah Buchanan William Andrews Ashley Evans Robert F Pass 《Journal of clinical virology》2006,35(3):338-342
BACKGROUND: An antibody method based on absorption of serum with cytomegalovirus (CMV) glycoprotein B (gB) was developed for detection of infection during clinical trials of CMV gB vaccine. Previous study showed that this method detected the antibody response to infection and was negative with vaccine induced immunity. OBJECTIVES: In an ongoing efficacy trial of CMV gB vaccine the ability of the gB-absorbed CMV IgG assay to detect CMV infection was assessed and compared with viral culture results. STUDY DESIGN: Two hundred and ninety two healthy, seronegative young women in a phase II, double-blind, placebo-controlled, clinical trial of recombinant CMV gB vaccine (sanofi pasteur) with MF59 adjuvant (Chiron) were randomized to receive CMV gB vaccine or placebo (1:1) on a 0, 1 and 6 month schedule. Participants were screened every 3 months for CMV infection using the gB-absorbed CMV IgG assay, and a subgroup was also screened for infection with viral cultures. Viral culture (urine, vaginal swab and saliva) was used to confirm CMV infection in all subjects with a positive gB-absorbed CMV IgG result. RESULTS: Evidence of CMV infection (gB-absorbed CMV IgG levels>or=5.0 AU/ml) was found in 23/292 (7.88%) study participants. The gB-absorbed CMV IgG levels of their first positive serum ranged from 15.7 to 251.0 AU/ml with a mean of 77.0 AU/ml and a median of 44.9 AU/ml. Cytomegalovirus was isolated from all 23 of them from culture specimens collected after their first positive gB-absorbed CMV IgG. The time to first CMV positive culture from first positive gB-absorbed CMV IgG ranged from 0 to 12 weeks with a median of 2 weeks. CONCLUSIONS: The gB-absorbed CMV IgG assay detects CMV infection in CMV gB vaccine clinical trials earlier and more rapidly than virus culture and does not reveal whether subjects received CMV gB vaccine or placebo. 相似文献
36.
P. J. Griffiths J. J. Duchateau Y. Maeda J. D. Potter C. C. Ashley 《Pflügers Archiv : European journal of physiology》1990,415(5):554-565
Intact muscle fibres fromBalanus nubilus develop tensions of up to 600 kN sd m−2 during electrical stimulation. The rise of tension occurs with a half-time (177 ms at 12° C) about fivefold longer than that
of tetanised frog muscle at the same temperature. The response of myofibrillar bundles to a rapid stretch resembles that of
frog muscle but has a yo value (i.e. the size of an instantaneous release necessary to just discharge tension) which is ca. 2.5 times smaller, and
phase 2 of the tension transient (the “quick phase”) occurs at a rate comparable to that of frog muscle. In contrast, the
ATPase activity (0.018 mmoles · kg wet weight−1 · s−1) of this preparation and its maximum shortening velocity (0.15–0.16 muscle lengths · s−1) are both at least fivefold slower than frog muscle. These findings can be accounted for by a cross-bridge cycle in barnacle
muscle in which events prior and subsequent to the tension generating step(s) occur at a rate at least fivefold slower than
comparable steps in frog muscle, but the step(s) associated with tension development occur at similar rates in the two preparations.
Since the rate of mechanical relaxation in barnacle muscle is modified in the presence of intracellular calcium buffers and
by depolarisation-induced elevation of the free calcium during the relaxation phase, it is proposed that the time course of
relaxation is not determined exclusively by the kinetics of the cross-bridge cycle, but is also dependent on the free calcium
concentration during relaxation. 相似文献
37.
Malyak Mark Smith Michael F. Abel Ashley A. Arend William P. 《Journal of clinical immunology》1994,14(1):20-30
Journal of Clinical Immunology - The objective of this study was to characterize interleukin-1 receptor antagonist (IL-1ra) and interleukin-1β (IL-1β) production by human peripheral blood... 相似文献
38.
Distal Proctocolitis and n-3 Polyunsaturated Fatty Acids (n-3 PUFAs): The Mucosal Effect In Situ 总被引:1,自引:0,他引:1
Almallah YZ Ewen SW El-Tahir A Mowat NA Brunt PW Sinclair TS Heys SD Eremin O 《Journal of clinical immunology》2000,20(1):68-76
It has been postulated that patients with ulcerative colitis (UC) have altered reactivity of gut-associated lymphoid tissue. In such cases there is intense infiltration of the mucosa with immune competent cells and associated tissue damage. We have shown previously that the dietary supplementation with the n-3 polyunsaturated fatty acids (n-3 PUFAs), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) results in significant systemic immune suppression. The aim of this study, therefore, was to evaluate the in situ effect of n-3 PUFAs on distal proctocolitis. Each patient received either fish oil extract (EPA 3.2 g, DHA 2.4 g) (n = 9) or sunflower oil (n = 9) daily in a double blind manner for six months. Monthly assessment included: (1) disease activity using clinical, sigmoidoscopic, and histological scores and (2) immunohistochemical analysis (immunoglobulins, CD profiles) of rectal biopsy specimens (before and after six months supplementation) using monoclonal antibodies and quantitative computer-assisted video image analysis. Prior to receiving supplementation, patients with proctocolitis (n = 18) showed significantly higher numbers of cells expressing CD3 (pan T cells) and HLA-DR and IgM containing cells compared with non-colitic controls (n = 8). Six months supplementation with n-3 PUFAs resulted in significant reduction in the number of cells expressing CD3 and HLA and the percentage of cells containing IgM. There was no significant change in the CD20 nor the percentage of IgG or IgA containing cells in either group of patients with procto-colitis. In patients receiving n-3 PUFA supplementation, there was improvement in the disease activity and histological scores, compared with pretreatment evaluation. This study has demonstrated both evidence of suppression of in situ immune reactivity and concurrent reduction in disease activity in patients with proctocolitis receiving n-3 PUFA supplementation. This may have important implication for therapy in patients with ulcerative colitis. 相似文献
39.
40.
Performance of a commercial, type-specific enzyme-linked immunosorbent assay for detection of herpes simplex virus type 2-specific antibodies in Ugandans 下载免费PDF全文
Laeyendecker O Henson C Gray RH Nguyen RH Horne BJ Wawer MJ Serwadda D Kiwanuka N Morrow RA Hogrefe W Quinn TC 《Journal of clinical microbiology》2004,42(4):1794-1796
Two hundred forty-eight human immunodeficiency virus (HIV)-positive and 496 HIV-negative subjects in Uganda were tested by HerpeSelect herpes simplex virus type 2 enzyme-linked immunosorbent assay (ELISA) and Western blotting to optimize the ELISA for use in this population. A higher index cutoff value was required for optimal sensitivity and specificity, and overall performance of the assay was not affected by HIV status. 相似文献