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排序方式: 共有693条查询结果,搜索用时 31 毫秒
51.
52.
D Saldanha N Kumar VSSR Ryali K Srivastava AA Pawar 《Medical Journal Armed Forces India》2009,65(2):108-112
Background
Serotonin plays an important role in treatment of depression. We evaluated the clinical correlates of plasma serotonin levels in depressed patients before and after treatment.Methods
Study sample comprised of 40 patients diagnosed on ICD-10 diagnostic criteria, and an equal number of healthy matched controls. Subjects were evaluated on Beck''s Depression Inventory (BDI) and Suicide Ideation Scale (SIS), before and after the treatment. Blood samples were collected from all the cases and controls before starting the antidepressant medication with selective serotonin reuptake inhibitors (SSRI''s). Serum serotonin levels were measured before and after treatment.Result
Significant differences in scores before and after the intervention on BDI, SIS and serotonin levels of cases and controls (p<.000) were noted. Correlation between the serum serotonin levels before and after the treatment, and between the rating scales did not reveal significant association (p > 0.05). Patients with suicidal intentions had lower levels of serotonin. The scores changed after intervention.Conclusion
Treatment with SSRI''s had shown significant changes in clinical conditions. However these changes did not relate significantly with serum serotonin levels.Key Words: Serotonin, Depression, Selective serotonin reuptake inhibitors 相似文献53.
EM Maier J Pongratz AC Muntau B Liebl U Nennstiel-Ratzel U Busch R Fingerhut B Olgemöller AA Roscher W Röschinger 《Clinical genetics》2009,76(2):179-187
Medium‐chain acyl‐CoA dehydrogenase deficiency (MCADD) represents a potentially fatal fatty acid β‐oxidation disorder. Newborn screening (NBS) by tandem mass spectrometry (MS/MS) has been implemented worldwide, but is associated with unresolved questions regarding population heterogeneity, burden on healthy carriers, cut‐off policies, false‐positive and negative rates. In a retrospective case‐control study, 333 NBS samples showing borderline acylcarnitine patterns but not reaching recall criteria were genotyped for the two most common mutations (c.985A>G/c.199C>T) and compared with genotypes and acylcarnitines of 333 controls, 68 false‐positives, and 34 patients. c.985A>G was more frequently identified in the study group and false‐positives compared to controls (1:4.3/1:2.3 vs. 1:42), whereas c.199C>T was found more frequently only within the false‐positives (1:23). Biochemical criteria were devised to differentiate homozygous (c.985A>G), compound heterozygous (c.985A>G/c.199C>T), and heterozygous individuals. Four false‐negatives were identified because our initial algorithm required an elevation of octanoylcarnitine (C8) and three secondary markers in the initial and follow‐up sample. The new approach allowed a reduction of false‐positives (by defining high cut‐offs: 1.4 μmol/l for C8; 7 for C8/C12) and false‐negatives (by sequencing the ACADM gene of few suspicious samples). Our validation strategy is able to differentiate healthy carriers from patients doubling the positive predictive value (42→88%) and to target NBS to MCADD‐subsets with potentially higher risk of adverse outcome. It remains controversial, if NBS programs should aim at identifying all subsets of all diseases included. Because the natural course of milder variants cannot be assessed by observational studies, our strategy could serve as a general model for evaluation of MS/MS‐based NBS. 相似文献
54.
Silvio ST Tafuri Domenico DM Martinelli Giovanni GC Caputi Annamaria AA Arbore Cinzia CG Germinario Rosa RP Prato 《BMC health services research》2009,9(1):100-5
Background
Mandatory vaccination has contributed to the success of immunisation programmes but voluntary vaccination allows people to be responsible for their own health. There are benefits from both policies and the arguments between them remain subject to debate within and without the scientific community, both nationally and internationally. The aim of this study is to assess the opinions of those who actually work in the Vaccination Service. 相似文献55.
56.
C. Boulanger NA Flavahan ZS Katusic K. Komori AA Vos and PM Vanhoutte MD Director 《Fundamental & clinical pharmacology》1990,4(5):525-538
Experiments were designed to determine the effect of CRL 41034, a buflomedil analogue, on the adrenergic responsiveness of canine veins. Rings of saphenous vein (without endothelium) were suspended for isometric tension recording in modified Krebs-Ringer bicarbonate solution at 37 degrees C. CRL 41034 produced a concentration-dependent inhibition of the contractions evoked by the alpha adrenergic agonists norepinephrine, phenylephrine and UK 14304 which was insensitive to the blockade of neuronal uptake by cocaine. CRL 41034 was more potent in inhibiting the concentration-dependent contractions evoked by UK 14304 than those by phenylephrine and the antagonism it caused against the response to UK 14304 fulfilled the criteria for competitivity. CRL 41034, at 10(-5) M significantly depressed, and at 10(-4) M abolished the contractions induced by electrical stimulation of the adrenergic nerves and those evoked by the indirect sympathomimetic amine tyramine. Strips of canine saphenous vein were superfused after incubation with [3H] norepinephrine. During sympathetic nerve activation, CRL 41304 increased the stimulation-evoked overflow of [3H] norepinephrine and 3-methoxy-4-dihydroxyphenylglycol; in the presence of rauwolscine the compound only increased the stimulation-evoked overflow of 3,4-dihydroxyphenylglycol. These experiments suggest that the major vascular effects of CRL 41034 in canine veins are blockade of alpha 2-adrenoceptors on vascular smooth muscle, and inhibition of prejunctional alpha 2-adrenoceptors on adrenergic nerve endings. 相似文献
57.
58.
Hinsch E; Ponce AA; Hagele W; Hedrich F; Muller-Schlosser F; Schill WB; Hinsch KD 《Human reproduction (Oxford, England)》1997,12(8):1673-1681
Binding of mammalian spermatozoa to the zona pellucida and the induction of
the acrosome reaction are prerequisites for successful oocyte
fertilization. It has been postulated that xenobiotics that are released in
the environment as well as exposure to pharmaceutical medications may be
associated with reproductive problems in men and wildlife. Examining
physiological and non-physiological effects of particular compounds on
sperm functions requires high quality in-vitro test systems. We established
a reliable combined in-vitro test system with bovine gametes and evaluated
if aliquots of pooled post-thaw spermatozoa are suitable for examining
essential sperm functions. Using cryopreserved semen, the PSA-FITC/Hoechst
33258 staining procedure was applicable to evaluate the acrosomal status
and cell viability. In the bovine hemizona assay, hemizona indices revealed
no differences between cryopreserved and fresh semen. Treatment of
post-thaw bovine spermatozoa with progesterone (1 microM or bovine
follicular fluid (20%) induced the acrosome reaction from 12% (untreated
spermatozoa) to 25% (P < 0.001) and to 22% [corrected] (P < 0.01),
respectively. Incubation of both compounds (1 microM progesterone and 20%
follicular fluid) raised the percentage of acrosome-reacted spermatozoa to
30% (P < 0001). Our results demonstrate that cryopreserved semen can be
integrated into an in-vitro screening model for reproductive toxicology
testing. Pooled, cryopreserved bovine spermatozoa will thus permit
reproducible experiments for clinical and basic science purposes and may
also be applicable for the human system.
相似文献
59.
60.
Cloning, functional activities and in vivo tissue distribution of rat NKR-P1+ TCR alpha beta + cells
Knudsen E; Seierstad T; Vaage JT; Naper C; Benestad HB; Rolstad B; Maghazachi AA 《International immunology》1997,9(7):1043-1051
We have successfully cloned nine NKR-P1+ TCR alpha beta + cells from PVG
rat spleens, utilizing murine macrophage inflammatory protein-1 alpha
(MIP-1 alpha) and IL-2. These clones are either double negative (DN,
CD4-CD8-), which included clones 3.31, 3.71, 4.19, 4.59 and 4.65, or single
positive (SP, CD4+CD8-), which included clones 1.64, 3.8, 3.76 and 3.78. No
CD8+ clone was recovered. All nine clones are restricted in terms of their
expression of the V beta antigens, since they express V beta 8.2 but not V
beta 8.5, V beta 10 or V beta 16. These clones are agranular and they fall
to generate NK or LAK activity upon incubation with IL-2, IL-12 or their
combination. On the basis of their production of intracellular cytokines
they can be divided into three categories: (I) SP clones (1.64, 3.8, 3.76
and 3.78) do not produce IL-2 or IL-4, but produce IFN-gamma and IL-12, and
they vary in their production of IL-1, RANTES or tumor necrosis factor
(TNF)-alpha; (II) DN clones 4.59 and 4.65 produce IL-1 alpha and IFN-gamma
only, and fall to produce other cytokines; and (III) DN clones 3.31, 3.71
and 4.19 produce IL-1 alpha, IL-1 beta, IL-2, IL-12, IFN-gamma, RANTES and
TNF-alpha. From all the clones examined only DN clones 3.31 and to a lesser
degree 4.19 produce IL-4. In vivo tissue localization of clones 3.8, 3.31
and 4.59 shows that these cells distribute into the liver and bone marrow
24 h post i.v. administration. Their accumulation in the liver and bone
marrow along with their ability to secrete various cytokines suggest that
these cells may influence the generation, differentiation or apoptosis of
immune or hematopoietic cells.
相似文献