Synergistic Protection of Danhong Injection (丹红注射液) and Ischemic Postconditioning on Myocardial Reperfusion Injury in Minipigs

Objective: To explore the synergistic protection of Danhong Injection (丹红注射液,DHI) and ischemic postconditioning on myocardial reperfusion injury in minipigs.Methods: Acute myocardial infarction model was made by balloon occlusion in left anterior descending coronary artery (LAD) of minipigs,and then postconditioning was simulated through inflation/deflation of the angioplasty balloon.Minipigs were divided into four groups: the sham operation group (SH group),the ischemia/reperfusion group (I/R group),the ischemic postconditioning group (POC group) and DHI combined with ischemic postconditioning group (PAD group,DHI 20 mL through ear vein),six in each group.After 24-h continuous observation,myocardial infarction size was assessed by triphenyltetrazolium staining (TTC).Morphological changes of ischemic myocardium were observed by light microscopy,and cardiomyocyte ultrastructure was studied with electron microscopy.The superoxide dismutase (SOD) and malondialdehyde (MDA) activity in heart homogenates were measured by a biochemical method.Results: The myocardial infarction size was smaller in the POC group than in the I/R group (0.26±0.02 vs.0.37±0.09,P0.05),and the PAD group (0.14±0.08) displayed a significantly reduced infarction size relative to the I/R group (P0.01) and POC group (P0.05).The damage of myocardial tissue was severe in the I/R group shown by light and electron microscopy: myocardial fibers disorder,sarcoplasmic dissolution,myofilament fracture,mitochondria swelling and even vacuolization formation and a large number of inflammatory cell infiltrations.Compared with the I/R group,reduction of reperfusion injury in the PAD group included more orderly arranged myocardial fibers,less infiltration of inflammatory cells and maintenance of mitochondrial integrity.Compared with the I/R group,the damage of myocardial tissue in the POC group was improved,but not as significant as that in the PAD group.SOD levels in the POC group and the PAD group were significantly higher than those in the I/R group (96.96±13.43,112.25±22.75 vs.76.32±10.63,P0.05),and MDA was significantly lower in the POC group and the PAD group compared to the I/R group (1.27±0.19,1.09±0.21 vs.1.47±0.16,P0.05).Conclusion: DHI and ischemic postconditioning show a synergistic cardioprotection on myocardial reperfusion injury in minipigs.     

骄傲公主和窝囊小子的心理游戏

她是骄傲的公主。她对他颐指气使。在她面前，他一贯窝囊。但是，有—天，他毫无征兆地离开了高高在上的她，让她陷入痛苦的深渊。[编者按]     

薏苡仁对扁平疣患者T淋巴细胞的影响

目的 观察薏苡仁对扁平疣患者皮损中T淋巴细胞的影响,初步探讨薏苡仁治疗扁平疣的可能作用机制.方法 收集扁平疣患者20例,经知情同意后予薏苡仁治疗,以薏苡仁粉30 g调糊口服,2次/d,同时外敷疣体,1次/晚,连续应用至疣体脱落或60 d止.采用免疫组织化学法对扁平疣患者治疗前后皮损中CD4+、CD8+T淋巴细胞水平进行检测,并与10例正常皮肤进行对照.结果 扁平疣患者治疗前较正常对照组CD4+/CD8+比值明显降低,差异有统计学意义(t=2.295,P<0.05);扁平疣患者治疗后较治疗前CD4+/CD8+比值明显升高,差异有统计学意义(t=2.121,P<0.05).结论 薏苡仁可能通过调节扁平疣患者皮损中T淋巴细胞的水平,从而增强机体免疫功能.     

通补法治疗冠心病国内RCT文献质量评价

目的 探讨通补法治疗冠心病(Coronary atherosclerotic heart disease,CHD)的疗效和安全性。方法 对中国知网全文数据库(China national knowledge infrastructure,CNKI)、中国生物医学文献数据库(Chinese biomedical literature database,CBM)、维普中文科技期刊全文数据库(VIP chinese scitech journal full-text database,VIP)和万方数据库建库至2021年2月文献进行检索。收集通补法治疗CHD随机对照试验(Randomized controlled trial,RCT),根据临床试验报告标准声明(2010版)(Consolidated standards of reporting trials,CONSORT)条目进行RCT文献质量评价,限定中文检索词为通阳、活络、补气、益气、冠心病、胸痹、胸痛、心痛。并总结纳入研究文献中结局指标的报告情况。结果纳入9篇RCT文献,包括849例CHD患者,文献发表的时间从2007年至2020年...     

基于“久卧伤气”探讨从气机防治急重症患者胃肠功能障碍

急重症患者在病程进展期间常常新发或合并急性胃肠功能障碍(AGD),导致原发病加重、延长住院时间从而增加医疗经济负担。因此类患者常常被动卧床改变了生理规律,结合“久卧伤气”理论,考虑可从调理气机论治预防及治疗急重症患者AGD的发生发展。笔者在临床治疗中从调理气机出发防治急重症患者AGD取得良好效果。     

原发性下腔静脉平滑肌肉瘤超声造影表现1例

患者女,56岁.因体检发现腹膜后肿块入院,无心悸、面色苍白、出汗、恶心、呕吐、恐惧焦虑、乏力等症状.查体:一般状况好,心肺未见明显异常.腹平软,双侧肋脊角对称,双侧肾区无压痛、叩击痛,右侧上腹部可触及一界限不清的肿块,活动度差.     

阻塞性睡眠呼吸暂停低通气综合征患儿主要照顾者负荷及照护知识需求分析

[目的]了解阻塞性睡眠呼吸暂停低通气综合征患儿居家照顾者照顾负荷及需求现状,探讨相关影响因素,为改善照顾者照顾行为提供依据。[方法]采用照顾负荷量表和照顾者照顾知识需求问卷对某三级甲等儿童专科医院耳鼻喉头颈外科427例阻塞性睡眠呼吸暂停低通气综合征患儿的主要照顾者427人进行调查。[结果]阻塞性睡眠呼吸暂停低通气综合征患儿的主要照顾者照顾负荷总分为37.78分±15.56分,负荷最大的前5项依次是睡眠受影响、休闲活动减少、生活习惯被打乱、压力增大、处于紧张状态。患儿年龄越小、照顾者为女性以及照顾者自身健康状况不佳均会增加照顾者的照顾负荷。照顾者对患儿术后疼痛干预、疾病饮食及用药指导方面的需求最为强烈。[结论]护理管理者需结合照顾者需求制定阻塞性睡眠呼吸暂停低通气综合征患儿主要照顾者的照顾方案,以提高其照顾水平,降低照顾负荷,从而加速患儿康复。     

醋酸棉酚——PVP固体分散体研究及体外杀精子活性的观察

本文对溶剂法制备醋酸棉酚—PVP固体分散体作了改进。以二氯甲烷为溶剂在45℃常压下完成,结果与Waller方法一致,并具有更大的安全性。经溶出实验筛选确定以1∶7醋酸棉酚—PVP配比可比Waller等人的1∶4配比提高药物溶出速率8倍,不同比例的固体分散体释药曲线均服从Q—t~(1/2)规律,且速度常数随固体分散体中PVP比例增加而线性递增。利用X射线衍射和~1HNMR研究了固体分散机制,认为是形成了间充型固体溶液,且PVP与醋酸棉酚之间有氢键结合。1∶7棉酚—PVP抑动精子实验结果表明10~(-3)M(0.052%)时20秒钟可抑制全部精子的向前运动。棉酚和醋酸棉酚结果一致,无统计学差异。PVP与生理盐水结果一致,表明PVP本身无抑制精子运动作用。     

中药带线棉栓对宫颈人类乳头状瘤病毒感染转阴的作用

目的 探讨中药带线棉栓对宫颈人类乳头状瘤病毒(human papillomavirus,HPV)感染转阴的干预作用.方法 将60例宫颈HPV感染的患者按治疗方法分为2组,治疗组(n=30)用中药带线棉栓(以鸦胆子、紫草、薏苡仁为主要成分)每次1枚宫颈用药,对照组(n=30)用奥平栓每次1粒(6万U·粒-1)宫颈用药,2组均隔天1次,7次为一疗程,每个月经周期用药一疗程,共三疗程.分别于疗程全部结束第1、3、6次月经干净后检测HPV的感染情况.结果 治疗后,治疗组和对照组第1-3次复查时HPV感染转阴率分别为56.7％、60.0％,66.7％、66.7％,73.7％、66.7％,2组比较差异均无统计学意义(均P＞0.05).结论 中药带线棉栓和奥平栓对宫颈HPV感染转阴的疗效相当.