pSVPoMcat微基因修饰雪旺细胞移植对脊髓损伤的修复作用

为观察pSVPoMcat微基因修饰雪旺细胞（SC）移植对脊髓损伤的修复作用，采用切割法制备脊髓半模断损伤模型（T8平面）。实验动物随机植入pSVPoMcat微基因修饰的SC（A组）、SC组（B组）和损伤对照组（C组），每组40只。术后应用联合行为记分（CBS）和皮层体感诱发电位（CSEP）动态观察大鼠功能恢复情况，应用原位杂交和免疫细胞化学方法观察胶质纤维酸性蛋白（GFAP）的表达。术后3月行MRI观察，并用免疫组化对神经轴突进行神经中丝（NF）染色。结果显示，A组能抑制大学损伤后的GFAP表达；MRI发现，A组大鼠脊髓损伤（SCI）区脊髓信号已基本恢复正常，B组未恢复正常，而C组SCI有软化灶形成。与NF染色发现一致。A、B两组潜伏时波幅呈恢复的趋势，与A组接近正常，与CBS结果一致。提示，pSVPoMcat微基因修饰SC移植能抑制SCI后GFAP的表达，促进神经轴突的生长并对神经功能恢复有明显的促进作用。     

儿童轻型脑外伤后腔隙性脑梗塞（附28例报告）

儿童外伤后脑梗塞多发生于基底节区,通常是由于中、重度颅脑外伤中的剪力所致,以轻型闭合性颅脑伤所致儿童基底节区腔隙性脑梗塞仍少见[1]。现就1992-01-1998-12我科收治的28例经CT证实的轻型闭合性颅脑伤后儿童基底节区腔隙性脑梗塞报告如下。临床资料     

移植pSVPoMcat微基因修饰雪旺细胞在鼠脊髓内长期存活及其基因表达

目的：观察人Po-5,flanking介导的21．5ku髓鞘碱性蛋白（myelin basic protiein,MBP)微基因（暂命名为pSVPoMcat)修饰雪旺细胞（SC）在鼠脊髓内存活及其基因表达。方法：120只大鼠分为3组,A组为pSVPoMcat微基因修饰SC移植组;B组为高纯化SC移植组;C组为脊髓损伤（SCI）＿对照组,伤后各组分别于2,4,8,12周（每组每次10只）,取移植区脊髓切片,进行S－100蛋白,MBP免疫细胞化学染色及地高辛(DIG)标记的hMBPF2 cDNA探针的原位杂交（ISH）测定。结果：伤后2,4,8,12周,A组S－100,MBP染色细胞和ISH细胞差异无统计学意义（P＜0．05）,其中ISH阳性细胞可见髓鞘形成,B组的S－100染色细胞逐步递减,差异有非常显著性意义（P＜0．01）,未发现MBP染色细胞以及ISH细胞,C组未发现任何阳性细胞,结论：pSVPoMcat微基因修饰SC在鼠损伤脊髓内能长期存活并表达外源基因,且有助于受伤脊髓的髓鞘形成。     

Effect of Microgene pSVPoMcat Implantation to Modify Genetically Schwann Cells on Central Conduction Function after Spinal Cord Injury

We have tested cortical somatasensory evoked potentials (CSEP)after spinal cord injury treated by pSVPoMcat modified Schwann cells implantation and the change of combined behavior score introduced by Gale et al.to explain its effect on central condution after experimental SCI.1 Materials and Methods1.1 SCI model and implantation process Adult SD rats( weighing 200－230g,with no gender regarded,provided by the animal Center of the West China University of medical)were made hemi－transe…     

微基因修饰雪旺氏细胞移植对脊髓损伤中枢传导功能的作用

Objective To approach the effect of microgene pSVPoMcat to modify genetically Schwann cells (SC) on central conduction functionafter spinal cord injury (SCI) . Method Experimental animals were divided into three groups: the group of microgene pSVPoMcat implanted to genetically modify SC (group A), SC implanted group (group B), and the control group (group C). The cortical somatasensory evoked potentials (CESP) and combined behavioral score (CBS) were continuous monitored from operation second week to twelfth week Result The result showed that the peak latency and peak amplitudes of group A, B have a recovery tendency and it was constant with CBS. Conclusion We concluded that the microgene pSVPoMcat to modify genetically Sc may play a promotion role in recovery of central conduction function after SCI.     

pSVPoMcat微基因修饰雪旺细胞脊髓内移植对损伤脊髓细胞的保护作用

Objective To study the protective effects of the intracord transplantation of microgene pSVPoMcat－ genetically－ modified Schwann cells (MSCs)on spinal cord injury (SCI).Method Rats with semi－ division(SD) of the spinal cord was divided into 4 groups.Group S consisted of the rats with SD treated with the transplantation of MSCs, Group B of the rats with SD treated with the transplantation of SCs without genetic modification,Group C of the rats with SD without treatment and Group D was the normal control. 8 hours after operation,the half of the rats of each group were killed and the injured segment of the spinal cord was resected to be examined with atomic absorption spectrophotometry . Another half of the rats of all the groups were examined with neurological function tests to have a combined behavioral score (CBS).Result There was a significant increase of water content and Na＋ and Ca2＋ ions and a decrease of K＋ and Mg 2＋ ions in the injured cord segment of Group C and a statistically significant recovery was observed in Group A. The intracord transplantation of pSVPoMcat genetically modidied SCs improved the neurological outcome of spinal cord injury.Conclusion Our findings indicate that intracord transplantation of pSVPoMcat－ genetically－ modified－ Schwanncells exerts protective effects on the injured segment of the spinal cord through the improvement of the internal ion environment of the spinal cord.     

Moderate hypothermia therapy for patients with severe head injury

ＳＤｅｐａｒｔｍｅｎｔｏｆＮｅｕｒｏｓｕｒｇｅｒｙ ,ＣｈｅｎｇｄｕＧｅｎｅｒａｌＨｏｓｐｉｔａｌｏｆＣｈｅｎｇｄｕＭｉｌｉｔａｒｙＣｏｍｍａｎｄ ,Ｃｈｅｎｇｄｕ 6 10 0 83,Ｃｈｉｎａ (ＣｈｅｎＬＧ ,ＰｉａｏＹＸ ,ＺｅｎｇＦＪ ,ＬｕＭ ,ＫｕａｎｇＹＱａｎｄＬｉＸ)ｉｎｃｅ 1990ｓ ,ｉｔｈａｓｂｅｅｎｐｒｏｖｅｄｂｙｃｌｉｎｉｃａｌｓｔｕｄｉｅｓａｔｈｏｍｅａｎｄａｂｒｏａｄｔｈａｔＭＨＴｉｓａｎｅｆｆｅｃｔｉｖｅａｎｄｓａｆｅｍｅｔｈｏｄ .1 3Ｔｏｂｅｔｔｅｒｕｎｄｅｒｓｔａｎｄｉｔｓｅｆｆｅｃｔａｎ…     

前瞻性研究脑室内出血的临床报告

目的 了解四川地区脑室内出血(IVH)的影响因素、病因、危险因素等,为IVH的临床研究提供依据.方法 采用前瞻性队列研究,以四川省3家三甲医院的住院患者为基础,进行多中心协作登记,收集包括人口学因素、IVH的危险因素、临床表现、辅助检查结果等数据.结果 纳入IVH患者660例,男416例(63.0％),女244例(37.0％);年龄17 ～ 93岁,平均(60.4±13.8)岁;原发性脑室内出血(PIVH)中高血压病31例(31.3％)、动脉瘤13例(13.1％)、血管畸形18例(18.2％)、烟雾病24例(24.2％)和未知病因13例(13.1％);而继发性脑室内出血(SIVH)中高血压病为391例(69.7％)、未知病因162例(28.9％)、动脉瘤、血管畸形和脑淀粉样血管病分别有3例(0.5％)、3例(0.5％)和2例(0.4％);入院时PIVH和SIVH组间GCS评分差异有统计学意义,P＜0.05;PIVH组47例(47.5％)手术治疗、32例(32.3)保守治疗,18例(18.2％)伽马刀治疗,另有2例(2.0％)介入治疗;SIVH组手术、保守和介入治疗分别是175例(31.2％)、383例(68.3％)和3例(0.5％),手术、保守和伽马刀治疗组间差异有统计学意义,P ＜0.05;多因素Logistic回归分析提示年龄、高血压是发生SIVH的独立危险因素.结论 本研究在国内外同类研究中样本量最大,研究质量较高,结论可靠,为IVH的临床研究设计提供了理论依据.     

神经内镜辅助显微血管减压术治疗面肌痉挛的临床研究

目的探讨神经内镜辅助下显微血管减压术（MVD）治疗原发性面肌痉挛（HFS）疾病的价值。方法收集泸州医学院附属医院神经外科2009年3月~2012年4月收治的原发性面肌痉挛患者62例,其中行神经内镜辅助下显微血管减压术的面肌痉挛患者32例（A组）,行传统显微血管减压术的面肌痉挛患者30例（B组）,比较两种治疗方法的手术治愈率、并发症的情况以及术后住院时间。结果责任血管发现：A组32例患者均发现责任血管,B组29例患者发现责任血管;治愈率：A组治愈率为96.9%（31例）,B组治愈率为90.0%（27例）;并发症发生率：A组为15.6%（5例）,B组为26.7%（8例）;住院时间：A组为（8.0±1.3）d,B组为（11.0±2.7）d;上述指标两组比较差异均有统计学意义（P〈0.05）。结论神经内镜辅助MVD治疗HFS能避免遗漏责任血管,减少对脑干、颅神经和重要血管的的牵拉损伤,对提高手术效果、减少术后并发症有重要价值。