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81.
Objective To analyze the proguostic factors for chronic lymphocytic leukemia (CLL) with typical and atypical immunophenotype. The parameters analyzed included sex, age, Binet stages, abso-lute lymphocyte count (ALC), immunoglobulin heavy-chain variable region (IgVH) gene mutation status, ZAP-70 protein, CD38 expression and cytogenetic aberrations. Methods According to the clinical guideline and scoring system for CLL in Britain, among 77 patients, 61 patients with score 5 called typical immunophe-notype CLL, 16 with score 4 or 3 were atypical immunophenotype CI,L. Multiparameter flow cytometry was employed for immunophenotypic analysis in 77 CLL patients for CD5, CD19, CD23, FMC7, slg, CD20, CD79h expression and ZAP-70 protein and CD38. IgVH mutation status was detected by multiplex RT-PCR and sequencing of the purified PCR amplification products. Fluorescence in situ hybridization (FISH) and a panel of probes were used to detect cytogenetic aberrations. Results There was no significant difference be-tween the two groups in sex, age, ZAP-70 and IgVH mutation status (P =0.398, P =0. 189, P =0.268 and P =0. 131, respectively). The incidence of ALC≥50 × 109/L, Binet B + C, CD38 ≥30% in atypical CLL patients(43.8%, 87.5% and 43.8%, respectively) were higher than that in typical group (16.4%, 36.1% and 16.4%, respectively) (P = 0. 026, P < 0. 01 and P = 0. 026, respectively). The proportion of typical patients (26. 8%) with a 13q14 deletion as sole abnormality was higher than that of atypical patients (7.6%), and that with deletion of 11q22 or 17p13 was lower than that of atypical patients (12.2% vs 46.2%) (P = 0. 022). Conclusion There were obvious differences between the typical immunophenotype CLL and atypical CLL in ALC, Binet stages, CD38 expression level and cytogenetic aberrations. 相似文献
82.
今报告从一急性肠炎患者粘液便中,分离出曼哈顿沙门菌。1.病例 患者女30岁,急性腹泻、恶心呕吐就医。粪便呈水样,可见脓细胞和红细胞,血中WBC12.6×10 /L。粪便培养得曼哈顿沙门菌。2.细菌学鉴定 接种SS、麦康凯及血琼脂平板,35℃孵育24h。SS平板上菌落呈中心黑色;麦康凯平板上菌落呈无色略混浊、边缘不整齐、表面粗糙;血平板上呈灰白色中等大小苗落。转种三糖铁,35℃过夜,上层红色,下层黑色,有动力,产H2S,不产气。3.生化反应 发酵葡萄糖产酸不产气,氧化酶、葡萄糖酸盐、吲哚、V-P、… 相似文献
83.
目的探讨消脱止治疗胫腓骨骨折所致肢体肿胀的疗效。方法回顾性分析120例胫腓骨骨折的临床资料,根据用药情况随机分为两组,观察组60例应用消脱止,对照组60例应用甘露醇,分别观察两组疗效。结果观察组显效41例,有效15例,有效率93.33%;对照组显效31例,有效9例,有效率66.67%,两组比较差异有统计学意义(P〈0.05)。结论消脱止能有效治疗胫腓骨骨折所致的肢体肿胀。 相似文献
84.
目的探讨多重荧光原位杂交(multiplex fluorescence in situ hybridization,M-FISH)及全染色体涂抹(whole chromosome painting,WCP)技术在骨髓增生异常综合征(myelodysplastic syndromes,MDS)复杂核型异常检测中的价值。方法对7例常规R显带具有复杂染色体异常的MDS患者应用M-FISH技术确定复杂染色体的重排及标记染色体的组成,识别微小易位。并进一步采用双色WCP技术验证M-FISH检测的结果。结果M-FISH不仅证实了R显带的结果,而且确定了R带核型分析没有确定的6种标记染色体、9种有不明来源的额外物质增加的染色体、5种衍生染色体的组成和来源及4种被忽略的微小易位。涉及17号染色体的异常及-5/5q-是MDS最为常见的两种染色体异常。WCP技术纠正了一些M-FISH漏检及误检的异常。结论M-FISH是明确复杂染色体异常的很有用的分子生物学工具,WCP是M-FISH技术的重要补充,R带核型分析结合分子细胞遗传学工具M-FISH和WCP可以更加准确地描述复杂染色体异常。 相似文献
85.
86.
Objective To analyze the proguostic factors for chronic lymphocytic leukemia (CLL) with typical and atypical immunophenotype. The parameters analyzed included sex, age, Binet stages, abso-lute lymphocyte count (ALC), immunoglobulin heavy-chain variable region (IgVH) gene mutation status, ZAP-70 protein, CD38 expression and cytogenetic aberrations. Methods According to the clinical guideline and scoring system for CLL in Britain, among 77 patients, 61 patients with score 5 called typical immunophe-notype CLL, 16 with score 4 or 3 were atypical immunophenotype CI,L. Multiparameter flow cytometry was employed for immunophenotypic analysis in 77 CLL patients for CD5, CD19, CD23, FMC7, slg, CD20, CD79h expression and ZAP-70 protein and CD38. IgVH mutation status was detected by multiplex RT-PCR and sequencing of the purified PCR amplification products. Fluorescence in situ hybridization (FISH) and a panel of probes were used to detect cytogenetic aberrations. Results There was no significant difference be-tween the two groups in sex, age, ZAP-70 and IgVH mutation status (P =0.398, P =0. 189, P =0.268 and P =0. 131, respectively). The incidence of ALC≥50 × 109/L, Binet B + C, CD38 ≥30% in atypical CLL patients(43.8%, 87.5% and 43.8%, respectively) were higher than that in typical group (16.4%, 36.1% and 16.4%, respectively) (P = 0. 026, P < 0. 01 and P = 0. 026, respectively). The proportion of typical patients (26. 8%) with a 13q14 deletion as sole abnormality was higher than that of atypical patients (7.6%), and that with deletion of 11q22 or 17p13 was lower than that of atypical patients (12.2% vs 46.2%) (P = 0. 022). Conclusion There were obvious differences between the typical immunophenotype CLL and atypical CLL in ALC, Binet stages, CD38 expression level and cytogenetic aberrations. 相似文献
87.
88.
为探讨慢性髓系白血病(CML)的细胞遗传学特点及临床意义,对362例CML患者采用24小时短期培养法制备骨髓染色体,用R显带技术进行染色体核型分析。将患者分为慢性期和急变期两组。结果表明:附加染色体异常、变异易位、Ph(-)bcr/abl(+)并伴有染色体异常者在两组中的比例分别为:70/268(26.1%)、19/268(7.1%)、4/268(1.5%);50/94(53.2%)、8/94(8.5%)、4/94、(4.3%)。362例标本中检出Ph阳性标本324例(89.5%),其中典型t(9;22)(q34;q11)易位297例(91.7%),变异易位27例(8.3%)。在27例变异易位中单纯变异易位13例,复杂变异易位13例,隐匿Ph1例。362例标本中共发现120例特殊核型异常。对上述异常分析显示,出现频率较高的数目异常有:+Ph:26例(21.7%);+8:12例(10.0%);+21:12例(10.0%);+19:9例(7.5%)。结构异常中以i(17q)最多,有16例(13.3%)。结论:与慢性期相比,急变期附加染色体、变异易位等异常率均明显增加,染色体核型分析有助于疾病进展的判断。 相似文献
89.
血小板抗体及淋巴细胞亚群检测在特发性血小板减少性紫癜中的诊断意义 总被引:3,自引:0,他引:3
目的 比较抗血小板特异性抗体、PAIgG及淋巴细胞亚群在特发性血小板减少性紫癜(ITP)及非免疫性血小板减少症中的水平,以评价其在ITP中的诊断价值.方法 用改良单克隆抗体特异性俘获血小板抗原法(MAIPA)检测患者血浆中抗血小板膜糖蛋白(GPⅡb/Ⅲa、GPⅠb和P-选择素)的特异性抗体.利用流式细胞术(FCM)检测患者外周血中PAIgG及淋巴细胞亚群.结果 ITP组MAIPA的阳性率为63.3%,非免疫性血小板减少组为阴性;PAIgG分别为73.3%、45%.淋巴细胞亚群中,ITP组CD3、CD4、CD4/CD8显著高于正常对照组,CD8、CD19则显著低于正常对照组.结论 抗血小板特异性自身抗体抗体对提高ITP的诊断有一定的实用价值,淋巴细胞亚群的变化能较好地反映ITP 的病理机制. 相似文献
90.
目的:探讨非清髓异基因造血干细胞移植(NST)治疗阵发性睡眠性血红蛋白尿(PNH)的疗效。方法:采用HLA全相合的同胞外周血造血干细胞移植,经环磷酰胺(CTX)、福达拉滨(Flud)和抗人淋巴细胞免疫球蛋白(ALG)方案进行NST预处理,环孢素A(CsA)、甲氨喋呤(MTX)和霉酚酸酯(MMF)三联预防移植物抗宿主病(GVHD)。结果:病例1在确诊后就进行了NST,输注CD34+细胞3.51×106/kg(按受者体重计算),第28天成功植入,无严重的GVHD表现及并发症;病例2在PNH确诊17年后进行NST,输注CD34+细胞数量2.2×106/kg,第60天成功植入,分别出现急性和慢性GVHD。结论:同胞全相合的NST是治疗PNH的有效方法,在PNH诊断早期进行NST以及回输足量的CD34+细胞是NST的有益因素。 相似文献