中西医结合治疗小儿功能性消化不良疗效观察

目的 观察中西医结合治疗小儿功能性消化不良的临床疗效。方法 将60例患儿随机分为两组，治疗组30例予健脾益气汤加吗丁啉口服，对照组30例单服吗丁啉，2周为1个疗程；比较两组疗效。结果 治疗组临床治愈17例，显效10例，有效3例，总有效率为100％；对照组临床治愈7例，显效14例，有效7例，无效2例，总有效率为93．33％。结论 健脾益气汤配合吗丁啉治疗小儿功能性消化不良较单用吗丁啉疗效为佳。     

EFFECT OF ELECTROACUPUNCTURE ON THE IMMUNOREACTIVITY OF FOCAL CUTANEOUS μ-OPIOID RECEPTOR IMMUNOREACTION-POSITIVE FIBERS IN ADJUVANT ARTHRITIC RATS

Objective: To study the effect of μ-opioid receptor (MOR) expression in the inflammatory skin tissue and the effect of electroacupuncture (EA) on the topical immunoreaction (IR) of MOR positive fibers in adjuvant arthritic (AA) rats. Methods: A total of 48 SD rats were randomized into control (n = 8), model (n = 10), focus-side-EA (n = 10), non-acupoint-EA (n = 10), and healthy-side-EA (n = 10) groups. AA model was established by subcutaneous injection of complete Freund's adjuvant (CFA, 50 μL) into the left hind paw. EA (4-16 Hz, 0.5-1.5 V) was applied to "Huantiao" (环跳 GB 30) and "Yanglingquan" (阳陵泉 GB 34) on the focus or healthy side and non-acupoints for 30 min. Non-acupoints used were the two sites 5 mm to GB 30 and GB 34 on the healthy side. The topical MOR IR-positive fibers in the dermal and subcutaneous tissues of the focus was stained with immunohistochemical method. The severity of pain was detected by foot (ankle-joint)-bending test. Results: Compared with model group, the "foot-bending test" score decreased significantly in focus-side-EA group on the 9th and 11th day (P<0.05) and in non-acupoint-EA group on the 8th, 9th and 11th d after injection of CFA (P < 0.05), indicating that EA of bilateral GB 30 and GB 34 and non-acupoints all can relieve pain. From the 13th day on, no significant differences were found in "foot-bending test" scores among the 3 EA groups and model group (P >0.05). In comparison with control group, the area values of MOR IR-positive nerve fibers in the focus tissue were significantly higher in 3 EA groups (P<0.05). The area values of MOR IR-positive nerve fibers in the focus in model group and 3 EA groups were significant higher than that in control group (P<0.05). Compared with model group, the area values of MOR IR-positive fibers in focus-side-EA group and healthy-side-EA group increased significantly (P<0.05); while those of MOR IR-positive fibers in non-acupoint-EA group and healthy-side-EA group were significantly lower than that in focus-side-EA group (P<0.05), and no significant differences were found among model group, healthy-side-EA group and non-acupoint-EA group in the area of MOR IR-positive fibers (P>0.05), indicating a stronger effect of EA of acupoints on the focus side. Conclusion: EA of GB 30 and GB 34 can relieve inflammatory pain and up-regulate the expression of MOR IR-positive fibers in the focal skin tissues in AA rats, exerting anti-inflammatory and analgesic effects.     

德国海军医学研究所介绍

德国海军医学研究所位于德国北部基尔市(Kiel)近郊Kronshagen的前基尔国防军医院内.该院不仅有海军医学研究所,还有国防军卫勤中心研究所、基尔专业医学中心和国防军基尔药房,基础设施为2家研究所共同使用.     

抓住机遇,促实验室建设与管理

本文从首都医科大学公共卫生与家庭医学学院的成立,结合新建实验室的特点,改建旧实验室模式,创建教学实验中心新体系;对实验室管理的规范化、科学化和现代化的探讨。     

CAG方案治疗初治老年急性髓性白血病的临床观察

随着人群寿命的延长,老年急性髓系白血病(AML)的发生率有所上升,老年人的生理学特点及常见的伴随疾病对白血病的治疗以及过程中出现的并发症多是不利的。一般完全缓解率为50%,中位数生存期仅5～6个月[1]。我院2003年1月～2005年6月应用CAG方案治疗老年急性髓系白血病9例,取得较     

脑血栓后遗症针刺康复治疗时诱导癫痫发作、并发多发性脑梗塞、脑心肺功能障碍死亡1例的机制浅析

在针刺疗法的异常情况中，晕针现象偶有发生。但针刺诱发癫痫发作者实属罕见。笔者受理一起因患脑血栓一年余仅左上肢瘫，无癫痫病史，于首次行针剌康复治疗中癫痫发作，并发多发性脑梗塞、脑心肺功能障碍死亡的案例。就其对针刺与癫痫发作、并发多发性脑梗塞的机制的点滴初识，予以浅析，可望同仁、专家点评。     

多层螺旋CT在肺动脉拴塞检查中的应用

目的 探讨多层螺旋CT增强扫描及血管三维成像对肺动脉栓塞的诊断价值。方法 对16例肺动脉栓塞患者进行多层螺旋CT增强扫描及肺动脉三维成像，以便更精确地直接观察血栓累及部位、形态特点及其他改变。结果 肺栓塞的直接征象：肺动脉内条状及斑片状充盈缺损，表现为部分性充盈缺损、完全性充盈缺损、轨道征、血管壁缺损；间接表现：肺门动脉增宽、肺梗死和肺纹理稀疏等。结论 由于多层螺旋CT对肺动脉栓塞检测的高敏感性和特异性，是一种能够可靠地用于临床怀疑急性肺栓塞病人的无创、安全、快速的检查方法。     

糖尿病大鼠畸变产物耳声发射的观察

目的观察糖尿病大鼠畸变产物耳声发射（distortion product otoacoustic emissions，DPOAE）的改变，探讨链脲霉素有无内耳毒性作用。方法24只SD大鼠随机分为糖尿病组、胰岛素治疗组和对照组，以链脲霉素诱发糖尿病，造模成功后以鱼精蛋白锌胰岛素控制血糖，分别于造模前、造模后3个月及5个月对3组大鼠进行DPOAE幅值测试，统计分析各组实验数据。结果糖尿病组大鼠发病3个月时DPOAE幅值出现下降，其中在1038、4126及5200Hz差异有统计学意义（P〈0．05）；发病5个月时糖尿病组大鼠DPOAE幅值在所有频率均出现显著下降，差异有统计学意义（P〈0．01）。胰岛素组与对照组DPOAE幅值在各个时期差异均无统计学意义。结论糖尿病早期即可出现DPOAE幅值下降，并会随着时间的延长逐渐加重。推测链脲霉素无明显内耳毒性作用。     

中药标准组分系统分离制备研究

以中药强极性组分、类组分、同系组分、微量组分为研究重点，采用新颖的分离表征策略和发展新型分离材料进行中药标准组分系统分离制备。发展硅胶基质的亲水性色谱填料分离中药强极性组分，以组合大孔树脂技术研究类组分，发展组合分子印迹技术分离同系组分，以差异化富集策略研究微量组分。同时，采用多模式、多柱色谱及其联用技术进行标准品的制备，建立标准品资源库，推动中药物质基础的研究。     

防风饮片的HPLC指纹图谱

防风为伞形科植物防风Saposhnikovia divaricata(Turcz·)Schischk未抽花茎的干燥根。目前国内外学者对防风的化学成分研究较详细,鉴别方法较多运用薄层色谱法[1]、高效液相色谱法[2]。但高效液相色谱法多为针对防风单一成分或几个成分的定性或定量检测[2]。但中药成分复杂,单纯测定少数几种有效成分或指标成分难以控制其内在质量。因此,逐步建立尽可能全面反映整体成分特征的HPLC指纹图谱[3],体现整体性和综合作用,可更好地评