微量淋巴细胞毒试验与PCR检测HLA—B27的比较

比较微量淋巴细胞毒试验与ＰＣＲ检测ＨＬＡ－Ｂ２７的方法，从中找出一种实用，可靠的方法为临床诊强直性脊柱炎提供依据。方法分别用微量淋巴细胞试验和ＰＣＲ对５０份临床怀疑ＡＳ的病人标本进行检测ＨＬＡ－Ｂ２７。结论无优质抗血清的情况下，可用ＰＣＲ代替微量淋巴细胞毒试验检测ＨＬＡ－Ｂ２７。     

甲氨喋呤不同方案治疗稳定型输卵管妊娠的临床观察

异位妊娠是妇产科常见急腹症之一,若不及时诊断和治疗可危及生命.阴道超声检查和血β-HCG测定使异位妊娠可以在未破裂前得到诊断,为保守治疗创造了条件.其中甲氨喋呤(MTX)是主要的保守治疗药物.现将我院MTX不同方案治疗稳定型输卵管妊娠的疗效分析如下.     

杂交捕获法和PCR-RDB法检测妇女HPV感染的比较

Objective To compare hybrid capture-Ⅱ(HC2)to polymerase chain reaction(PCR)-reverse dot-blot(RDB)for detecting HPV infection in women.Methods HC2 method and PCR-RDB were applied to detecting human papillomaviral infection in female genital duct,and the test results were analyzed.Results Concordant results were found in 81.14%(400/493)samples(Kappa=0.63;95%CI,0.57-0.69).The high risk type HPV positive rate detected by PCR-RDB were 1.60%(3/187),29.85%(20/67),69.88%(58/83),86.52%(77/89)and 91.04%(61/67),respectively,when the HC2 RLU/CO of samples were＜1.00,1.00-9.99,10.00-99.99,100.00-999.99 and≥1000.00.Statistical differences were found among the five groups(χ2=289.3,P＜0.01).Cross reactions were found between HC2 high risk probe and HPV53,66,11,cp8304 and other genotypes.Conclusion There is good concordance between HC2 and PCR-RDB,though cross reaction exists in HC2.Relatively speaking,there are more influential factors for PCR-RDB.     

药学监护在芬太尼联合丙泊酚麻醉中的临床价值

目的：分析药学监护在芬太尼联合丙泊酚麻醉中的临床价值。方法将120例手术者随机分为研究组和对照组各60例。患者均使用芬太尼联合丙泊酚进行局麻或全麻，在此基础上研究组由临床药师进行麻醉监控，观察2组中局麻手术患者的疼痛分级，全麻手术患者睁眼时间和问答切题用时，以及不良反应发生率。结果2组局麻手术患者疼痛同一等级比较差异有统计学意义（P＜0．05）。研究组睁眼时间和问答切题时间均短于对照组，差异均有统计学意义（P＜0．05），研究组不良反应发生率为3．3％低于对照组的13．3％，差异有统计学意义（P＜0．05）。结论药学监护在芬太尼联合丙泊酚麻醉中效果明显，值得临床推广使用。     

杂交捕获法和PCR-RDB法检测妇女HPV感染的比较

Objective To compare hybrid capture-Ⅱ(HC2)to polymerase chain reaction(PCR)-reverse dot-blot(RDB)for detecting HPV infection in women.Methods HC2 method and PCR-RDB were applied to detecting human papillomaviral infection in female genital duct,and the test results were analyzed.Results Concordant results were found in 81.14%(400/493)samples(Kappa=0.63;95%CI,0.57-0.69).The high risk type HPV positive rate detected by PCR-RDB were 1.60%(3/187),29.85%(20/67),69.88%(58/83),86.52%(77/89)and 91.04%(61/67),respectively,when the HC2 RLU/CO of samples were＜1.00,1.00-9.99,10.00-99.99,100.00-999.99 and≥1000.00.Statistical differences were found among the five groups(χ2=289.3,P＜0.01).Cross reactions were found between HC2 high risk probe and HPV53,66,11,cp8304 and other genotypes.Conclusion There is good concordance between HC2 and PCR-RDB,though cross reaction exists in HC2.Relatively speaking,there are more influential factors for PCR-RDB.     

胃癌穿孔的诊断与术式选择探讨

目的:探讨胃癌穿孔的临床特点和不同术式的选择指征.方法:回顾性分析21例胃癌穿孔病人临床表现、术式选择和预后.结果:术前、术中诊断胃癌穿孔18例,误诊3例.行胃癌根治9例,姑息性胃癌切除5例,穿孔修补7例,3种术式术后各死亡1例,中位生存期分别为26.5、15.8和5.7个月.结论:提高术前、术中确诊率,据病人情况选择合适的术式是保障患者生命安全、延长患者生存时间、提高生存质量的关键.     

大骨节病人的电声阻抗测定

大骨节病是一种原因不明的地方性、慢性退行性骨关节病。据莫东旭等人研究表明大骨节病主要侵害软骨内化型骨骼的透明软骨,据目前剖检和活检材料看到,除下颌关节未见到明显改变外,其它含有软骨组织的骨骼也有改变。据此推     

人类抗精子抗体对精子功能的影响：1.人类抗精子抗体对精子穿越宫颈…

为探讨抗精子抗体对精子穿越宫颈粘液能力的影响，本文用１５份含高滴度抗精子抗体的血清配成不同稀释度的血清标本，用间接精子免疫珠结合试验测定各血清标本的抗体滴度，并用精子宫颈粘液穿透试验检测不同滴度抗精子抗体对精子穿越宫颈粘液能力的影响。