EGFL7基因沉默对裸鼠胃癌血管生成的影响

Objective To investigate the effect of short hairpin RNA (shRNA) targeting at epidermal growth factor-1 ike domain 7 ( EGFL7 ) gene on angiogenesis of stomach carcinoma and its mechanism in nude mice.Methods shRNA targeting at EGFL7 gene was constructed and transfected into SGC-7901 cells (pshEGFL7 group), meanwhile, the cells transfected with vector plasmids were used as control group.Positive clones were selected and the transplanted tumor animal models were constructed in nude mice, and the growth and volume of tumors were observed.After 8 weeks, EGFL7 mRNA and protein in transplanted tumor tissues were detected.Moreover, Anti-CD34, VEGF and TSP1 were stained by the immuno- chemistry method, and MMP-2 and TIMP2 mRNA were detected by RT-PCR.Results EGFL7 mRNA was significantly down regulated in pshEGFL7 group.In psh EGFL7 group, the tumor volume was 1.86 ± 0.65 cm3,MVD was 20.84 ± 6.38, while in control group, the tumor volume was 4.86 ± 1.15 cm3, MVD was 39.48±9.01.In EGFL7 group, TSP1 protein presented positive, and VEGF protein presented weakly positive or negative.The expression of MMP-2 mRNA decreased, TIMP2 mRNA increased in pshEGFL7 group, and it was significantly different compared with control group( P ＜0.01 ).Conclusion RNA interference targeting at EGFL7 gene can balance TSP1/VEGF, through regulating the expression of MMP-2/TIMP2 to impair angiogenesis of stomach cancer in nude mice.     

妊娠期乳腺癌患者治疗期间真实体验的质性研究

目的了解妊娠期乳腺癌患者治疗期间的真实感受,为妊娠期乳腺癌患者实施针对性心理护理提供参考。方法采用质性研究的现象学研究方法,对13例妊娠期乳腺癌患者进行深入访谈。结果共提炼出4个主题:对疾病认知缺乏,消极的情绪变化,母亲角色不完整性的反应,对疾病信息的需求迫切。结论妊娠期乳腺癌患者对疾病相关知识缺乏,存在消极情绪。护理人员应深入了解妊娠期乳腺癌患者的真实内心感受,从而制定切合需要的护理服务策略,以满足患者护理需求。     

EGFL7基因沉默对裸鼠胃癌血管生成的影响

Objective To investigate the effect of short hairpin RNA (shRNA) targeting at epidermal growth factor-1 ike domain 7 ( EGFL7 ) gene on angiogenesis of stomach carcinoma and its mechanism in nude mice.Methods shRNA targeting at EGFL7 gene was constructed and transfected into SGC-7901 cells (pshEGFL7 group), meanwhile, the cells transfected with vector plasmids were used as control group.Positive clones were selected and the transplanted tumor animal models were constructed in nude mice, and the growth and volume of tumors were observed.After 8 weeks, EGFL7 mRNA and protein in transplanted tumor tissues were detected.Moreover, Anti-CD34, VEGF and TSP1 were stained by the immuno- chemistry method, and MMP-2 and TIMP2 mRNA were detected by RT-PCR.Results EGFL7 mRNA was significantly down regulated in pshEGFL7 group.In psh EGFL7 group, the tumor volume was 1.86 ± 0.65 cm3,MVD was 20.84 ± 6.38, while in control group, the tumor volume was 4.86 ± 1.15 cm3, MVD was 39.48±9.01.In EGFL7 group, TSP1 protein presented positive, and VEGF protein presented weakly positive or negative.The expression of MMP-2 mRNA decreased, TIMP2 mRNA increased in pshEGFL7 group, and it was significantly different compared with control group( P ＜0.01 ).Conclusion RNA interference targeting at EGFL7 gene can balance TSP1/VEGF, through regulating the expression of MMP-2/TIMP2 to impair angiogenesis of stomach cancer in nude mice.     

天荔汤对糖尿病大鼠早期肾脏高滤过的影响

目的观察天荔汤对糖尿病大鼠早期肾脏高滤过的影响。方法随机取8只大鼠作为正常组,余52只大鼠用四氧嘧啶诱导糖尿病大鼠模型,以血糖值在13～25 mmol/L者为成模,按血糖高低随机分为模型组、西药组和天荔汤高、低剂量组,每组8只,并给与相应药物。4周后检测大鼠血糖、肾质量、体质量、肾小球滤过率（GFR）及血浆一氧化氮（NO）、内皮素（ET）变化。结果与正常组相比,模型组血糖、尿量、肾质量、体质量、GFR、肾质量与体质量比值（KW/BW）等指标明显增高（P〈0.01）。天荔汤高、低剂量组及西药组均能降低GFR,高剂量组与西药作用相当。天荔汤高剂量组能明显降低肾质量（P〈0.01）,并接近正常。天荔汤高剂量组KW/BW指数明显降低,优于天荔汤低剂量组与西药组（P〈0.01）。结论天荔汤可明显改善糖尿病大鼠肾脏高滤过反应。     

应用数字减影血管造影与B超判断上肢高压电烧伤患者的血管损伤情况

目的　比较数字减影血管造影 (DSA)与B超在判断上肢高压电烧伤血管损伤中的作用。　方法　选择 19例上肢高压电烧伤患者的尺、桡动脉作为烧伤组 ,术前应用DSA及B超检测技术 ,观察患肢腕部创面及创面近端 5、10、15cm处尺、桡动脉的内膜、管壁厚度、管腔内径、血流量以及血栓形成等情况 ;以 12位正常人的尺、桡动脉作为对照组。术中 :结合DSA和B超检查结果 ,综合分析并判断尺、桡动脉损伤程度 ,出现栓塞或管壁坏死者予以切除并进行组织病理学检查。　结果DSA检测显示烧伤组患者 14条尺动脉、11条桡动脉出现异常 ,主要变化有栓塞形成、管腔狭窄、血流缓慢等 ,尺动脉损伤程度重于桡动脉。B超显示烧伤组 19条尺动脉和 16条桡动脉出现异常 ,主要表现为血管内膜粗糙不平、水肿或脱落 ,管壁增厚 ,管腔狭窄或串珠样改变 ,血流量减少 (P <0.0 5～ 0.0 1)其中创缘近端 5cm的尺动脉血流量 (31.6 0± 13.90 )ml/min,明显低于对照组 (47.70± 9.6 0 )ml/min(P <0.0 5)。术中探查及组织病理学检查结果与DSA、B超诊断相符。　结论　判断上肢高压电烧伤后的血管损伤情况可采用B超为主、DSA为辅的方法 ,以提高手术探查的精确度。     

经胸大肌外下方前锯肌筋膜切口在硅凝胶假体乳房再造中的应用

目的:探讨胸大肌外下方前锯肌筋膜切口在硅凝胶假体乳房再造中的应用效果。方法:回顾2014年2月—2015年1月124例行乳腺切除加假体植入乳房再造的乳腺癌患者临床资料,其中传统的乳腺癌改良根治术23例,保留皮肤的全乳切除术22例,保留乳头乳晕的全乳切除术79例,患者均应用胸大肌外下方前锯肌筋膜切口行硅凝胶假体乳房再造。分析手术情况,术后效果,并发症及远期随访结果。结果:124例患者均成功完成手术,手术时间为65～120 min,平均(83.62±14.18)min;术中出血量为45～105 mL,平均(78.14±17.47)mL。术后外观评价"优良"率为82.25%(102/124)。术后血清肿7例,经引流后好转;切口愈合不佳,伤口裂开5例,经抗炎,换药,再次缝合后好转;无假体取出患者。随访22～50个月,失访9例,失访前均无瘤生存;骨转移5例,肺转移1例,肝转移1例,无局部复发患者。结论:胸大肌外下方前锯肌筋膜切口在硅凝胶假体乳房再造中安全有效,操作简便,适合推广。     

某地区7岁以下儿童地中海贫血与G6PD缺乏症的流行病学调查

目的通过对仫佬族地区7岁以下儿童地中海贫血与G6PD缺乏症的流行病学调查,了解本地区仫佬族、壮族、汉族的7岁以下儿童两种疾病发生率、有无民族及性别的差异,为本地区地贫和G6PD缺乏症的遗传咨询及预防提供基础资料,提高出生人口素质。方法选出符合HB<110 g/L,MCV<80 fL,MCH<27 pg,MCHC<32%,RDW>17%标准的本地区仫佬族、壮族、汉族的7岁以下儿童共4372例,取静脉血1.5 mL,对其进行血常规、红细胞脆性试验,血细胞分析阳性者2215行血红蛋白电泳、G6PD检测,整个检测过程和标准安全、无不良反应。结果所调查的地区地中海贫血274例(12.4%),男童β地贫119例(8.7%)、α地贫54例(3.9%),女童β地贫62例(7.4%)、α地贫39例(4.5%),男女童地贫阳性率无统计学意义(P>0.05)。仫佬族、壮族、汉族的β地贫阳性率分别为7.5%、10.5%、5.0%,α地贫阳性率分别为4.2%、4.2%、4.1%。G6PD缺乏症发生率7.1%,男童发生率8.1%,女童发生率5.5%,男童大于女童,男女童差异有统计学意义。结论仫佬族地区7岁以下儿童在地中海贫血与G6PD缺乏症的发病中,存在民族、性别的差异,此项调查为仫佬族地区首次开展两种遗传病的大规模筛查,遗传咨询及预防提供基础资料,提供出生人口素质,有较好的社会效益,前景广阔。     

C臂导向经皮穿刺注射臭氧治疗腰椎间盘突出症58例疗效观察

目的探讨经皮穿刺注射臭氧治疗腰椎间盘突出症的疗效。方法 58例患者在C臂引导下行病变椎间盘盘内穿刺,盘内和椎旁注射臭氧,术后随访6～12个月。结果根据改良的Macnab疗效评定标准评定:优27例,良19例,可10例,差2例,优良率为79.3%(46/58),总有效率为96.6%(56/58)。结论 C臂导向经皮穿刺注射臭氧治疗腰椎间盘突出症,效果满意,且操作方便,对病人创伤小,医疗成本低,值得推广。