胃癌中肿瘤相关纤维母细胞蛋白和VEGF的表达及意义

目的 观察胃癌中肿瘤相关纤维母细胞蛋白SMA及VEGF的表达、并探讨其与胃癌侵袭性的关系.方法 应用免疫组织化学S-P方法检测57例胃癌和10例正常胃黏膜组织中纤维母细胞平滑肌肌动蛋白(SMA)、血管内皮生长因子(VEGF)的表达,并探讨其在胃癌侵袭转移的意义.结果 胃癌组织纤维母细胞中SMA阳性表达率为94.74%,高于正常胃黏膜组(P<0.05),VEGF阳性表达率为89.47%,高于正常胃黏膜组(P<0.05),且随着浸润深度的增加、临床分期的进展和淋巴结转移的出现,VEGF和纤维母细胞中SMA的表达增强.结论 VEGF、SMA在胃癌组织中存在高水平表达,二者在胃癌的浸润转移中起重要作用.     

p53促凋亡刺激蛋白与肿瘤的相关性研究进展

p53是一个重要的细胞凋亡因子.近些年英国科学家发现的p53凋亡刺激蛋白(apoptosis stimulating protein of p53,ASPP)是一种新的蛋白家族,包括三个成员:ASPP1、ASPP2和iASPP,三者都能与p53特异性结合,促进或抑制p53诱导细胞凋亡的功能.本文现就ASPPs与肿瘤的相关性作一综述.     

Using Intra-operative GeneSearchTM BLN Assay to Detect Breast Cancer Metastases in Sentinel Lymph Nodes - Results from a single institute in China

Background: Sentinel lymph node (SLN) biopsy has become a common procedure for early breast cancer patients. The GeneSearchTM Breast Lymph Node (BLN) Assay is a real-time RT-PCR assay for the detecting nodal metastases larger than 0.2mm. Methods: The SLNs from 90 consecutive patients were identified and dissected, and then sectioned along the short axis into multiple blocks. Intra-operatively, the odd blocks were tested by BLN assay and the even ones were used for frozen section (FS), while all the blocks were evaluated by touch imprint cytology (TIC). Post-operatively, the remaining tissues were assessed by histological evaluation. Results: 189 SLNs were tested by BLN assay. The sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) were 88.9%, 97.4%, 88.9% and 97.4%, respectively, for BLN assay, 75%, 100%, 100% and 94.4%, respectively, for FS, and 63.9%, 100%, 100% and 92.2%, respectively, for TIC. The sensitivity of BLN assay was higher than that of TIC (P = 0.01) and FS (P = 0.13). When assessing the nodes with micro-metastases, BLN assay had a significant higher sensitivity than FS (P=0.023) and TIC (P=0.005). Conclusion: The GeneSearchTM BLN Assay is an accurate and rapid intra-operative assay for breast SLNs and it is suitable for application in general medical practice.